| Project/Area Number |
20790868
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Psychiatric science
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| Research Institution | Tokyo Metropolitan Organization for Medical Research |
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Principal Investigator |
TANAKA Susumu Tokyo Metropolitan Organization for Medical Research, 東京都精神医学総合研究所, 研究員 (30399472)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ナルコレプシー / ORX / IGFBP3 / NR6A1 / 転写制御 / オレキシン / Nur77 / 睡眠 |
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| Research Abstract |
In this study, I examined the activities of transcriptional modulations of two narcolepsy candidate genes, IGFBP3 and NR6A1, against ORX transcription. (1)IGFBP3 and NR6A1 expression were found in hypothalamic ORX neurons by immunehistochemical studies. (2)By using reporter assay, IGFBP3 and NR6A1 modulated ORX promoter activity in several cell lines. (3)IGFBP3 overexpression mice showed the reduction of hypothalamic ORX expression and modulated sleep conditions. I also found NR6A1 overexpression modulated ORX expression, however the mice number examined have not reached statistically enough yet.
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