Radio-chemical and biological mechanisms in cell inactivation under hypoxia

• Project/Area Number: 20790923
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: HIRAYAMA Ryoichi National Institute of Radiological Sciences, 重粒子医科学センター, 研究員 (90435701)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 細胞致死 / 直接作用 / 間接作用 / OHラジカル / DMSO / X線 / DNA損傷修復欠損細胞 / DSB修復欠損細胞 / 酸素効果 / 低酸素

Research Abstract

We examined the contributions of OH radical mediated indirect action of X-irradiation on cell killing of CHO, AA8, xrs-6 and 51D1 cells under oxic and hypoxic conditions. The contributions of indirect action for xrs-6 cells under both conditions showed lower than that for CHO, AA8 and 51D1 cell lines.

Research Products

• [Journal Article] Direct and indirect actions to high-LET radiations. (2010)
  • Author(s): Ryoichi Hirayama, Yoshitaka Matsumoto, Yuki Kase, Miho Noguchi, Nobuhiro Takase, Akiko Uzawa, Atsushi Ito, Naruhiro Matsufuji, Koichi Ando, Ryuichi Okayasu, Yoshiya Furusawa
  • Journal Title: NIRS-KI Joint Symposium on Ion-Radiation Sciences NIRS-M-235 Pages: 155-162
• [Journal Article] 細胞やDNAへの影響から見たトラック構造 (2010)
  • Author(s): 平山亮一、伊藤敦、古澤佳也
  • Journal Title: 放射線化学 No.89 Pages: 9-12
  • Peer Reviewed
• [Journal Article] Radioprotection by DMSO in Nitrogen Saturated Mammalian Cells Exposed to Helium Ion Beams. (2009)
  • Author(s): Ryoichi Hirayama, Yoshitaka Matsumoto, Yuki Kase, Miho Noguchi, Koichi Ando, Atsushi Ito, Ryuichi Okayasu, Yoshiya Furusawa
  • Journal Title: Radiation Physics and Chemistry 78 Pages: 1175-1178
  • Peer Reviewed
• [Journal Article] Contributions of Direct and Indirect Actions in Cell Killing by High LET Radiations. (2009)
  • Author(s): Ryoichi Hirayama, Atsushi Ito, Masanori Tomita, Teruyo Tsukada, Fumio Yatagai, Miho Noguchi, Yoshitaka Matsumoto, Yuki Kase, Koichi Ando, Ryuichi Okayasu, Yoshiya Furusawa
  • Journal Title: Radiation Research 171 Pages: 212-218
  • Peer Reviewed
• [Journal Article] Radioprotection by DMSO in Nitrogen Saturated Mammalian Cells Exposed to Helium Ion Beams (2009)
  • Author(s): Ryoichi Hirayama, Yoshitaka Matsumoto, Yuki Kase, Miho Noguchi, Koichi Ando, Atsushi lto, Ryuichi Okayasu, Yoshiya Furusawa
  • Journal Title: Radiation Physics and Chemistry 78 Pages: 1175-1178
  • Peer Reviewed
• [Journal Article] Contributions of Direct and Indirect Actions in Cell Killing by High LET Radiations 枠 (2009)
  • Author(s): Ryoichi Hirayama, Atsushi Ito, Masanori Tomita, Teruyo Tsukada, Fumio Yatagai, Miho Noguchi, Yoshitaka Matsumoto, Yuki Kase, Koichi Ando, Ryuichi Okayasu, Yoshiya Furasawa
  • Journal Title: Radiation Research 171 Pages: 212-218
  • Peer Reviewed
• [Presentation] 重粒子線の放射線作用による高RBEのメカニズム (2010)
  • Author(s): 平山亮一、松本孔貴、野口実穂、高瀬信宏、加瀬優紀、鶴岡千鶴、松藤成弘、伊藤敦、安藤興一、岡安隆一、古澤佳也
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-22
• [Presentation] 重粒子線誘発OHラジカルの細胞致死影響と酸素効果の機構解明 (2010)
  • Author(s): 平山亮一
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都(受賞講演)
  • Year and Date: 2010-10-22
• [Presentation] 重粒子線誘発OHラジカルの細胞致死影響と酸素効果の機構解明 (2010)
  • Author(s): 平山亮一
  • Organizer: 日本放射線影響学会第53回大会(受賞講演)
  • Place of Presentation: 京都
  • Year and Date: 2010-10-21
• [Presentation] Direct and indirect actions to high-LET radiations. (2010)
  • Author(s): Ryoichi Hirayama, Yoshitaka Matsumoto, Yuki Kase, Miho Noguchi, Nobuhiro Takase, Akiko Uzawa, Atsushi Ito, Naruhiro Matsufuji, Koichi Ando, Ryuichi Okayasu, Yoshiya Furusawa
  • Organizer: NIRS-KI Joint Symposium on Ion-Radiation Sciences
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2010-09-10
• [Presentation] Direct and indirect actions to high-LET radiations (2010)
  • Author(s): Ryoichi Hirayama, Yoshitaka Matsumoto, Yuki Kase, Miho Noguchi, Nobuhiro Takase, Akiko Uzawa, Atsushi Ito, Naruhiro Matsufuji, Koichi Ando, Ryuichi Okayasu, Yoshiya Furusawa
  • Organizer: NIRS-KI Joint Symposium on Ion-Radiation Sciences
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2010-09-10
• [Presentation] The type of DSB by direct action of X-rays is different comparison with the DSB induced by indirect action. (2010)
  • Author(s): Ryoichi Hirayama, Atsushi Ito, Yoshitaka Matsumoto, Akiko Uzawa, Miho Noguchi, Ryuichi Okayasu, Yoshiya Furusawa
  • Organizer: 38th Annual Meeting of the European Radiation Research Society
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2010-09-06
• [Presentation] The type of DSB by direct action of X-rays is different comparison with the DSB induced by indirect action (2010)
  • Author(s): Ryoichi Hirayama, Atsushi Ito, Yoshitaka Matsumoto, Akiko Uzawa, Miho Noguchi, Ryuichi Okayasu, Yoshiya Furusawa
  • Organizer: 38th Annual Meeting of the European Radiation Research Society
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2010-09-06
• [Presentation] 低酸素下における高LET放射線誘発OHラジカルの細胞致死作用 (2010)
  • Author(s): 平山亮一、松本孔貴、野口実穂、高瀬信宏、加瀬優紀、鶴岡千鶴、松藤成弘、伊藤敦、安藤興一、岡安隆一、古澤佳也
  • Organizer: 第49回日本医学放射線学会生物部会学術大会
  • Place of Presentation: 札幌
  • Year and Date: 2010-07-09
• [Presentation] Contributions of direct and indirect actions in cell killing by high-LET radiations under hypoxic condition (2010)
  • Author(s): Ryoichi Hirayama, Yoshitaka Matsumoto, Miho Noguchi, Nobuhiro Takase, Yuki Kase, Chizuru Tsuruoka, Naruhiro Matsufuji, Atsushi Ito, Koichi Ando, Ryuichi Okayasu, Yoshiya Furusawa
  • Organizer: the 49th Annual Meeting of the Particle Therapy Co-Operative Group (PTCOG 49)
  • Place of Presentation: Maebashi
  • Year and Date: 2010-05-20
• [Presentation] Contributions of direct and indirect actions in cell killing by high-LET radiations under hypoxic condition (2010)
  • Author(s): Ryoichi Hirayama, Yoshitaka Matsumoto, Miho Noguchi, Nobuhiro Takase, Yuki Kase, Chizuru Tsuruoka, Naruhiro Matsufuji, Atsushi Ito, Koichi Ando, Ryuichi Okayasu, Yoshiya Furusawa
  • Organizer: the 49th Annual Meeting of the Particle Therapy Co-Operative Group (PTCOG 49)
  • Place of Presentation: 前橋
  • Year and Date: 2010-05-20
• [Presentation] DNA二本鎖切断はLET増加に伴い増加するのか?減少するのか?:(3)DSB生成収率の増減ファクターは何か? (2009)
  • Author(s): 平山亮一、古澤佳也
  • Organizer: 日本放射線影響学会第52回大会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-13
• [Presentation] DNA二本鎖切断はLET増加に伴い増加するのか?減少するのか?:(3)DSB生成収率の増減ファクターは何か? (2009)
  • Author(s): 日平山亮一、古澤佳也
  • Organizer: 日本放射線影響学会第52回大会
  • Place of Presentation: 広島市南区民文化センター(広島)
  • Year and Date: 2009-11-13
• [Presentation] INDUCTION OF DNA DSB AND ITS REJOINING IN THE CLAMPED AND NON-CLAMPED TUMORS AFTER CARBON ION BEAMS COMPARED WITH X RAYS (2009)
  • Author(s): Ryoichi Hirayama, Akiko Uzawa, Yoshitaka Matsumoto, Miho Noguchi, Yuki Kase, Atsushi Ito, Ryuichi Okayasu, Sachiko Koike, Koichi Ando, Yoshiya Furusawa
  • Organizer: MICROS 2009 15th International Symposium on Microdosimetry
  • Place of Presentation: Verona, Italy
  • Year and Date: 2009-10-29
• [Presentation] 高線量域におけるRBEとOER (2009)
  • Author(s): 平山亮一
  • Organizer: 平成21年度京都大学原子炉実験所専門研究会
  • Place of Presentation: 大阪
  • Year and Date: 2009-10-18
• [Presentation] 細胞の生き死にから見たトラック構造 (2009)
  • Author(s): 平山亮一、古澤佳也、岡安隆一
  • Organizer: 高LET放射線研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-07-30
• [Presentation] 移植腫瘍のX線と重粒子線に対する感受性の比較-RBEとOERを中心に- (2009)
  • Author(s): 平山亮一、古澤佳也、鵜澤玲子、高瀬信宏、小池幸子、安藤興一、増永慎一郎、松本孔貴、加瀬優紀、野口実穂、伊藤敦、岡安隆一
  • Organizer: 第48回日本医学放射線学会生物部会学術大会
  • Place of Presentation: 富山国際会議場(富山)
  • Year and Date: 2009-07-10
• [Presentation] NHEJ修復欠損xrs6細胞における放射線作用の細胞致死効果 (2008)
  • Author(s): 平山亮一、古澤佳也、伊藤敦、野口実穂、松本孔貴、鵜澤玲子、安藤興一、岡安隆一
  • Organizer: 日本放射線影響学会第51回大会
  • Place of Presentation: 北九州
  • Year and Date: 2008-11-21
• [Presentation] LET dependence of oxidative DNA base damage in 2-deoxyguanine aqueous solution irradiated with heavy ions (2008)
  • Author(s): Ryoichi Hirayama, Yoshiya Furusawa, Chieko Murayama, Yohsuke Kusano, Atsushi Ito
  • Organizer: The 2nd Asia-Pacific Symposium on Radiation Chemistry (APSRC-2008)
  • Place of Presentation: Tokyo
  • Year and Date: 2008-09-01
• [Presentation] Radioprotection by DMSO in Nitrogen Saturated Mammalian Cells Exposed to Helium Ion Beams (2008)
  • Author(s): Ryoichi Hirayama, Yoshitaka Matsumoto, Yuki Kase, Miho Noguchi, Koichi Ando, Atsushi Ito, Ryuichi Okayasu, Yoshiya Furusawa
  • Organizer: The 2nd Asia-Pacific Symposium on Radiation Chemistry (APSRC-2008)
  • Place of Presentation: Tokyo
  • Year and Date: 2008-08-30
• [Presentation] トラック構造と生物学的エンドポイントの関係 (2008)
  • Author(s): 平山亮一、加瀬優紀、伊藤敦、古澤佳也
  • Organizer: 平成20年度弥生研究会~放射線化学とその周辺から~
  • Place of Presentation: 茨城
  • Year and Date: 2008-07-29
• [Presentation] Contribution of indirect action of X-irradiation under oxic and hypoxic conditions in different radiosensitivity cells (2008)
  • Author(s): Ryoichi Hirayama、Yoshiya Furusawa、Atsushi Ito、Miho Noguchi、Yoshitaka Matsumoto、Akiko Uzawa、Koichi Ando、Ryuichi Okayasu
  • Organizer: 10th International Workshop Radiation Damage to DNA
  • Place of Presentation: Fukushima
  • Year and Date: 2008-06-12
• [Remarks] ホームページ
  • URL: http://www.nirs.go.jp/index.shtml
• [Remarks] 平成22年度日本放射線影響学会奨励賞平山亮一:重粒子線誘発OHラジカルの細胞致死影響と酸素効果の機構解明、2010.10.21、京都
• [Remarks] Travel Support Award. Ryoichi Hirayama : The type of DSB by direct action of X-rays is different comparison with the DSB induced by indirect action. 38th Annual Meeting of the European Radiation Research Society, 2010.09, Stockholm, Sweden
• [Remarks] The Outstanding Poster Award. Ryoichi Hirayama : Contributions of direct and indirect actions in cell killing by high-LET radiations under hypoxic condition. 49th Annual Meeting of the Particle Therapy Co-Operative Group (PTCOG 49) 2010.05.21, Maebashi
• [Remarks] Financial Assistance for Young Scientists. Ryoichi Hirayama : INDUCTION OF DNA DSB AND ITS REJOINING IN THE CLAMPED AND NON-CLAMPED TUMORS AFTER CARBON ION BEAMS COMPARED WITH X RAYS. 15th International Symposium on Microdosimetry (Micros 2009) 2009.10, Verona, Italy
• [Remarks] 第48回日本医学放射線学会生物部会学術大会大会長賞平山亮一:移植腫瘍のX線と重粒子線に対する感受性の比較-RBEとOERを中心に-、第48回日本医学放射線学会生物部会学術大会、2009.07.10、富山
• [Remarks] Young Investigator Awards. Ryoichi Hirayama : Contribution of indirect action of X-irradiation under oxic and hypoxic conditions in different radiosensitivity cells. 10th International Workshop on Radiation Damage to DNA, 2008.06, Fukushima
• [Remarks] 平成22年度日本放射線影響学会評議員
• [Remarks] 若手放射線生物学研究会副運営委員(2010.01-2010.12)
• [Remarks] 平成21年度日本放射線影響学会評議員
• [Remarks] 平成21年度日本放射線影響学会学会連携検討委員会委員
• [Remarks] 平成21年度日本放射線影響学会「評議員選挙」選挙管理委員会委員長
• [Remarks] 若手放射線生物学研究会運営委員(2009.01-2009.12)
• [Remarks] The 2nd Asia-Pacific Symposium on Radiation Chemistry (APSRC-2008), EXECUTIVE COMMITTEE、2008.08
• [Remarks] 平成20年度日本放射線影響学会評議員
• [Remarks] 平成20年度日本放射線影響学会学会連携検討委員会委員
• [Remarks] 若手放射線生物学研究会運営委員(2008.01-2008.12)
• [Remarks] 表彰
• [Remarks] 1.平成22年度日本放射線影響学会奨励賞平山亮一:重粒子線誘発OHラジカルの細胞致死影響と酸素効果の機構解明、京都、2010.10.21、受賞講演有り
• [Remarks] 2.Travel Support Award.Ryoichi Hirayama : The type of DSB by direct action of X-rays is different comparison with the DSB induced by indirect action.38th Annual Meeting of the European Radiation Research Society, Stockholm, Sweden, 2010.09
• [Remarks] 3.The Outstanding Poster Award. Ryoichi Hirayama : Contributions of direct and indirect actions in cell killing by high-LET radiations under hypoxic condition.49th Annual Meeting of the Particle Therapy Co-Operative Group (PTCOG 49), Maebashi, Japan, 2010.05.21
• [Remarks]
  • URL: http://www.nirs.go.jp/db/seikadb/Welcome.php
• [Remarks] 表彰
• [Remarks] 1)Financial Assistance for Young Scientists. 15th International Symposium on Microdosimetry (Micros 2009), Verona, Italy 2009.10.29
• [Remarks] 2)第48回日本医学放射線学会生物部会学術大会大会長賞第48回日本医学放射線学会生物部会学術大会、富山、2009.07.10