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Establishmentof a strategy to regulate alloreactive T cells by CD14^+monocyte-derived endothelial-like cells for applying in organ transplantation

Research Project

Project/Area Number 20790931
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionHiroshima University

Principal Investigator

TANAKA Yuka  Hiroshima University, 大学院・医歯薬学総合研究科, 助教 (90432666)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords臓器移植 / 免疫寛容 / 内皮細胞
Research Abstract

We have established a strategy to inhibit alloreactive T cells without nonspecific immunosuppressants by using immune-regulatory endothelial-like cells (ELCs) derived from peripheral blood CD14^+ monocytes. We have also attempted to establish an in vivo mouse model to investigate the strategy to regulate alloreactive T cells (i.e. liver endothelia-chimeric Rag-2/γ-chain double knockout mice reconstructed with syngeneic T cells). The ELCs regenerated from peripheral blood CD14+ monocytes expressed MHC class II, CD80 and CD86 together with PDL-1 and ILT-3 (inhibitory molecules against T cells). Adding the stimulator-type ELCs into the culture of mixed reaction assay resulted in the significant inhibition of T cell proliferation in the stimulator-specific fashion. These findings suggest that adoptive transfer with the ELCs regenerated from donor peripheral blood CD14^+ monocytes specifically induces tolerance among alloreactive T cells in organ transplantation.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (6 results)

All 2010 2009

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (3 results)

  • [Journal Article] Immunological property of antibodies against N-glycolylneuraminic acid epitopes in cytidine monophospho-N-acetylneuraminic acid hydroxylase-deficient mice2010

    • Author(s)
      Tahara H, Ide K, Basnet NB, Tanaka Y, Matsuda H, Takematsu H, Kozutsumi Y, Ohdan H
    • Journal Title

      J Immunol 15;184(6)

      Pages: 3269-3275

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination of the precursor frequency and the reaction intensity of xenoreactive human T lymphocytes2010

    • Author(s)
      Tahara H, Ide K, Basnet NB, Tanaka Y, Ohdan H
    • Journal Title

      Xenotransplantation (In press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination of the precursor frequency and the reaction intensity of xenoreactive human T lymphocytes.2010

    • Author(s)
      Tahara H, et al.
    • Journal Title

      Xenotransplantation (掲載決定)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 肝類洞内皮細胞の抗原特異的免疫寛容誘導能の解析2010

    • Author(s)
      番匠谷将孝、尾上隆司、五十嵐友香、田中友加、井手健太郎、伊禮俊充、田原裕之、田澤宏文、田代裕尊、大段秀樹
    • Organizer
      第46回日本肝臓学会総会
    • Place of Presentation
      山形
    • Year and Date
      2010-05-27
    • Related Report
      2009 Final Research Report
  • [Presentation] Optimization of immunosuppressive therapy on the basis of immune monitoring by a multiparametric mixed lymphocyte reaction assay reduces infectious complications and mortality in living-donor liver transplantation2010

    • Author(s)
      Yuka Tanaka, Hirotaka Tashiro, Hideki Ohdan
    • Organizer
      American Transplant Congress 2010
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2010-05-04
    • Related Report
      2009 Final Research Report
  • [Presentation] 当院における肝移植後の免疫寛容をめざした試み~肝類洞内皮細胞の抗原特異的免疫寛容誘導能~2009

    • Author(s)
      番匠谷将孝
    • Organizer
      第27回日本肝移植研究会
    • Place of Presentation
      東レ総合研修センター(静岡県)
    • Year and Date
      2009-07-10
    • Related Report
      2009 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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