A functional analysis of stabilizing factor BAP1 of cancer-restraining gene BRCA1

• Project/Area Number: 20790939
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General surgery
• Research Institution: St.Marianna University School of Medicine
• Principal Investigator: NISHIKAWA Hiroyuki St.Marianna University School of Medicine, 医学(系)研究科(研究院), 研究技術員 (90387077)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 癌遺伝子 / 乳癌 / 遺伝子

Research Abstract

BAP1 interacts with BARD1.
BAP1 inhibits the E3 ligase activity of BRCA1 by interfering the BRCA1/BARD1 RING heterodimer formation.
BAP1 is capable of deubiquitinating BRCA1 autoubiquitinated.
BAP1 and BRCA1/BARD1 coordinately regulate ubiquitination during the DNA damage response and the cell cycle.

Research Products

• [Journal Article] Recruitment of phosphorylated NPM1 to sites of DNA damage through RNF8-dependent ubiquitin conjugates. (2010)
  • Author(s): Koike A, Nishikawa H, Wu W, Okada Y, Venkitaraman AR, Ohta T.
  • Journal Title: Cancer Res. 70(17) Pages: 6746-6756
  • Peer Reviewed
• [Journal Article] HERC2 is an E3 ligase that targets BRCA1 for degradation. (2010)
  • Author(s): Wu W, Sato K, Koike A, Nishikawa H, Koizumi H, Venkitaraman AR, Ohta T.
  • Journal Title: Cancer Res. 70(15) Pages: 6384-92
  • Peer Reviewed
• [Journal Article] Recruitment of phosphorylated NPM1 to sites of DNA damage through RNF8-dependent ubiquitin conjugates (2010)
  • Author(s): Ayaka Koike
  • Journal Title: Cancer Research Volume: 70(17) Pages: 6746-6756
  • Peer Reviewed
• [Journal Article] BRCA1-associated protein 1 interferes with BRCA1/BARD1 RING heterodimer activity. (2009)
  • Author(s): Nishikawa H, Wu W, Koike A, Kojima R, Gomi H, Fukuda M, Ohta T.
  • Journal Title: Cancer Res. 69(1) Pages: 111-119
  • Peer Reviewed
• [Journal Article] Overexpression of heat shock protein 27 in squamous cell carcinoma of the uterine cervix : a proteomic analysis using archival formalin-fixed, paraffin-embedded tissues. (2009)
  • Author(s): Ono A, Kumai T, Koizumi H, Nishikawa H, Kobayashi S, Tadokoro M.
  • Journal Title: Hum Pathol. 40(1) Pages: 41-49
  • Peer Reviewed
• [Journal Article] BRCA1-Associated Proteinl Interferes with BRCAIBARD1 RING Heterodimer Activity (2009)
  • Author(s): Hiroyuki Nishikawa
  • Journal Title: Cancer Research 69(1) Pages: 111-119
  • Peer Reviewed