Induction of transplant tolerance by regulatory T cell in rat liver transplantation

• Project/Area Number: 20790945
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Chiba University
• Principal Investigator: TAKAYASHIKI Tsukasa Chiba University, 医学部・附属病院, 助教 (30456024)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 肝臓外科学 / 制御性T細胞 / 肝移植 / 移植寛容 / 移植・再生医療 / 免疫学 / 外科

Research Abstract

In present study, we established transplant tolerance in orthotropic rat liver transplantation and transplant tolerance was transferred adoptively by peripheral lymphocyte, within regulatory T cells. CD4 (+) T cells or CD8 (+) T cells from peripheral lymphocyte of long term survivor were adoptive transferred to naive rat after cell sorting and we revealed that both groups were able to induce transplant tolerance in rat liver transplantation. These results suggested that regulatory T cells were existed not only in population of CD4 (+) CD25 (+) T cells but in CD8 (+) T cells.

Research Products

• [Journal Article] Significance of repeated resection for recurrent intrahepatic cholangiocarcinoma. (2009)
  • Author(s): Ohtsuka M, Kimura F, Shimizu H, Yoshidome H, Kato A, Yoshitomi H, Frukawa K, Mitsuhashi N, Takeuchi D, Takayashiki T, Suda K, Miyazaki M.
  • Journal Title: Hepatgastroenterology. 56 Pages: 1-5
• [Journal Article] Usefulness of intraoperative fluorescence imaging to evaluate local anatomy in Hepatobiliary surgery. (2008)
  • Author(s): Mitsuhashi N, Kimura F, Shimizu H, Yoshidome H, Ohtsuka M, Kato A, Yoshitomi H, Nozawa S, Frukawa K, Takeuchi D, Takayashiki T, Suda K, Igarashi T, Miyazaki M.
  • Journal Title: J Hepatobiliary Pancreat Surg. 15 Pages: 508-514
  • NAID: 10024305895
• [Presentation] ラット心移植モデルにおけるドナー抗原刺激による免疫寛容維持 (2009)
  • Organizer: 第109回日本外科学会定期学術集会
  • Year and Date: 2009-04-04
• [Presentation] ラット心移植モデルにおけるドナー抗原刺激による免疫寛容維持 (2009)
  • Author(s): 高屋敷吏
  • Organizer: 第109回日本外科学会定期学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2009-04-04
• [Presentation] ラット肝移植自然生着モデルにおけるドナー特異的抗原刺激の関与 (2008)
  • Organizer: 第108回日本外科学会定期学術集会
  • Year and Date: 2008-05-17
• [Presentation] ラット肝移植自然生着モデルの移植寛容におけるドナー特異的抗原刺激の関与 (2008)
  • Author(s): 高屋敷吏
  • Organizer: 第108回日本外科学会定期学術集会
  • Place of Presentation: 長崎
  • Year and Date: 2008-05-17