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The role of Chemokine receptor CXCR3 in colon cancer metastasis

Research Project

Project/Area Number 20790953
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionKyoto University

Principal Investigator

KAWADA Kenji  Kyoto University, 医学研究科, 助教 (90322651)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords大腸癌 / ケモカイン受容体 / 転移 / ケモカイン
Research Abstract

The SW480 cell line was derived from a primary Duke B colon cancer, whereas the SW620 cell line was derived from a lymph node metastasis of the same patient. Both CXCR3 and CXCR4 were much higher expressed in the SW620 than in the SW480. Cell migration, invasion and proliferation were induced by interaction of ligands with CXCR3 or CXCR4 in both cell lines. Importantly, CXCR4-induced migration and invasion were promoted when CXCR3 was pre-activated with its ligands in advance. These results suggest that CXCR3 can play a role in colon cancer metastasis by cooperating with CXCR4 synergistically. We established three stable cell lines using miRNA; CXCR3 knockout, CXCR4 knockout and CXCR3/CXCR4 double knockout cell lines. We have compared the metastatic potential of the three cell lines with the control cells by rectal transplantation model in nude mice.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (13 results)

All 2010 2009 2008 Other

All Journal Article (7 results) (of which Peer Reviewed: 2 results) Presentation (2 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Hepatic stellate cells promote liver metastasis of colon cancer cells by the action of SDF-1/CXCR4 axis.2009

    • Author(s)
      Ryo Matsusue, Hajime Kubo, Shigeo Hisamori, Kae Okoshi, Hidekazu Takagi, Koya Hida, Keiko Nakano, Atsushi Itami, Kenji Kawada, Satoshi Nagayama, Yoshiharu Sakai
    • Journal Title

      Ann Surg Oncol Vol16

      Pages: 2645-2653

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 大腸癌の浸潤・転移におけるケモカインシグナル2009

    • Author(s)
      河田健二、武藤誠
    • Journal Title

      分子細胞治療 Vol8

      Pages: 31-35

    • Related Report
      2009 Final Research Report
  • [Journal Article] Hepatic stellate cells promote liver metastasis of colon cancer cells by the action of SDF-1/CXCR4 axis2009

    • Author(s)
      松末亮
    • Journal Title

      Annals of Surgical Oncology 16

      Pages: 2645-2653

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 大腸癌の浸潤・転移におけるケモカインシグナル2009

    • Author(s)
      河田健二
    • Journal Title

      分子細胞治療 8

      Pages: 31-35

    • Related Report
      2009 Annual Research Report
  • [Journal Article] 大腸癌の浸潤・転移におけるケモカイン・シグナル2009

    • Author(s)
      河田健二, 武藤誠
    • Journal Title

      分子細胞治療

    • Related Report
      2008 Annual Research Report
  • [Journal Article] 大腸癌リンパ節転移におけるケモカイン受容体CXCR3発現の意義2008

    • Author(s)
      河田健二、細木久裕、坂井義治、武藤誠
    • Journal Title

      Biotherapy Vol22

      Pages: 71-79

    • NAID

      110006330419

    • Related Report
      2009 Final Research Report
  • [Journal Article] 大腸癌リンパ節転移におけるケモカイン受容体CXCR3発現の意義2008

    • Author(s)
      河田健二、細木久裕、坂井義治、武藤誠
    • Journal Title

      Biotherapy 22

      Pages: 71-79

    • NAID

      110006330419

    • Related Report
      2008 Annual Research Report
  • [Presentation] 大腸癌の浸潤・転移におけるケモカインシグナル2010

    • Author(s)
      河田健二
    • Organizer
      第110回日本外科学会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-04-09
    • Related Report
      2009 Final Research Report
  • [Presentation] 大腸癌の浸潤・転移におけるケモカイン・シグナル2010

    • Author(s)
      河田健二
    • Organizer
      第110回 日本外科学会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-04-09
    • Related Report
      2009 Annual Research Report
  • [Book] Cancer drug discovery and development- Chemokine receptors in cancer2009

    • Author(s)
      Makoto Taketo, Kenji Kawada
    • Publisher
      Springer
    • Related Report
      2009 Final Research Report
  • [Book] Cancer Drug Discovery and Development-Chemokine Receptors in Cancer2009

    • Author(s)
      武藤誠、河田健二
    • Publisher
      Springer
    • Related Report
      2009 Annual Research Report
  • [Book] Cancer Drug Discovery and Development-Chemokine Receptors in Cancer2009

    • Author(s)
      Taketo Makoto、Kawada Kenji
    • Publisher
      Springer
    • Related Report
      2008 Annual Research Report
  • [Remarks] ヒト臨床検体をもちいた更なる解析のために「大腸癌におけるケモカイン受容体発現の意義」「大腸癌におけるケモカイン受容体CXCR4の免疫染色に関する臨床的意義」として京都大学医学部の倫理委員会の承認を受けており、京都大学医学部消化管外科のホームページにも公表している。

    • URL

      http://www.kuhp.kyoto-u.ac.jp/%7Egisurg/

    • Related Report
      2009 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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