Expression of sonic hedgehog signaling for regulation of liver regeneration after massive hepatectomy of the rat liver
| Project/Area Number |
20790958
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | The University of Tokushima |
|---|
Principal Investigator |
MORINE Yuji The University of Tokushima, 医学部・歯学部附属病院, 助教 (60398021)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SHIMADA Mitsuo 徳島大学, 大学院・ヘルスバイオサィエンス研究部, 教授 (10216070)
IMURA Satoru 徳島大学, 医学部・歯学部附属病院, 助教 (90380021)
IKEGEMI Toru 徳島大学, 医学部・歯学部附属病院, 助教 (80432938)
KENEMURA Hirofumi 徳島大学, 医学部・歯学部附属病院, 診療助教 (20467814)
NAKAMURA Takanori 香川大学, 医学部, 教授 (70183887)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 肝臓外科学 / ソニックヘッジホッグ / 肝再生 / ソニックヘッジホッジ / アクチビン / フォリスタチン / 大量肝切除 |
|---|
| Research Abstract |
Background : This study was conducted to clarify the possible functional involvement of Hh signaling, especially in Sonic hedgehog (Shh) in liver regeneration. Methods : Six-week-old male Wister rats were performed 70% hepatectomy (70%Hx, n=6) and 90% hepatectomy (90%Hx, n=6). The liver/body weight ratio and immunohistochemistry by anti-PCNA, anti-Shh and anti-Gli-1 antibody were evaluated their expression in each groups. Results : Hepatocytes were strongly stained by PCNA at 24 hr after Hx. Regarding non-parenchymal cells, they gradually stained by PCNA from 24hr to 72hr after Hx. Shh and Gli-1 expressions of hepatocytes were higher in 24 hr than other time point. In non-parenchymal cells, they gradually increased after Hx, and Shh and Gli-1-positive non-parenchymal cells mainly existed in zone I. Conclusions : Those findings indicated some relations between Shh pathway and liver regeneration. It is possible that they induced efficient and smooth hepatic reconstruction.
|
Report
(3 results)
Research Products
(16 results)
