• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of molecular marders associated with lymph node metastasis in pancreatic cancer

Research Project

Project/Area Number 20790966
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionWakayama Medical University

Principal Investigator

HIRONO Seiko  Wakayama Medical University, 医学部, 学内助教 (60468288)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsリンパ節転移 / 遺伝子 / 膵癌 / 外科
Research Abstract

Lymph node metastasis (LNM) is the most important prognostic factor in patients undergoing surgical resection of pancreatic ductal adenocarcinoma (PDAC). In this study, we aimed to identify molecular markers associated with LNM in PDAC using genome-wide expression profiling. In this study, laser microdissection and genome-wide transcriptional profiling were used to identify genes that were differentially expressed between PDAC cells with and without LNM obtained from 20 patients with PDAC. Immunohistochemical staining was used to confirm the clinical significance of these markers in an additional validation set of 43 patients. In the results, microarray profiling identified 46 genes that were differently expressed between PDAC with and without LNM with certain significance. Four of these biomarkers were validated by immunohistochemical staining for association with LNM in PDAC in an additional validation set of patients. In 63 patients with PDAC, significant LNM predictors in PDAC elucidated from multivariate analysis were low expression of AP2α (P=0.012) and high expression of MUC17 (P=0.0192). We demonstrate that AP2α and MUC17 may serve as potential prognostic molecular markers for LNM in patients with PDAC.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (14 results)

All 2010 2009 2008

All Journal Article (4 results) (of which Peer Reviewed: 2 results) Presentation (10 results)

  • [Journal Article] Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome-wideexpression profiling.2010

    • Author(s)
      Hirono S, Yamaue H, Hoshikawa Y, Ina S, Tani M, Kawai M, Ushijima M, Matsuura M, Saiki Y, Saiura A, Yamamoto J, Miki Y, Noda T.
    • Journal Title

      Cancer Sci 101

      Pages: 259-66

    • NAID

      10027200498

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome-wide expression profiling.2010

    • Author(s)
      Seiko Hirono
    • Journal Title

      Cancer Sci 101

      Pages: 259-266

    • NAID

      10027200498

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Treatment Strategy of Intraductal Papillary Mucinous Neoplasm of the Pancreas Based on Malignant Predictive Factors2009

    • Author(s)
      Seiko Hirono
    • Journal Title

      Arch Surg 144

      Pages: 1-6

    • Related Report
      2008 Annual Research Report
  • [Journal Article] A Collision tumor composed of cancers of the bile duct and ampulla of vater-immunohistochemical analysis of a rare entity of double cancer2008

    • Author(s)
      Seiko Hirono
    • Journal Title

      Hepatogastroenterology 55

      Pages: 2373-2377

    • Related Report
      2008 Annual Research Report
  • [Presentation] 網羅的遺伝子発現解析を用いた膵癌リンパ節転移予測マーカーの同定;AP2α・MUC172009

    • Author(s)
      廣野誠子
    • Organizer
      第40回日本膵臓学会
    • Place of Presentation
      東京
    • Year and Date
      2009-07-30
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] AP2α・MUC17は膵癌リンパ節転移予測マーカーである2008

    • Author(s)
      廣野誠子
    • Organizer
      第21回日本バイオセラピィ学会
    • Place of Presentation
      東京
    • Year and Date
      2008-11-18
    • Related Report
      2009 Final Research Report
  • [Presentation] 網羅的遺伝子発現プロファイリンよる膵癌リンパ節転移予測因子の同定2008

    • Author(s)
      廣野誠子
    • Organizer
      第46回癌治療学会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-11-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] AP2α・MUC17は膵癌リンパ節転移予測マーカーである2008

    • Author(s)
      廣野誠子
    • Organizer
      第21回日本バイオセラピー学会
    • Place of Presentation
      東京
    • Year and Date
      2008-11-08
    • Related Report
      2008 Annual Research Report
  • [Presentation] 網羅的遺伝子発現プロファイリングによる膵癌リンパ節転移予測因子の同定2008

    • Author(s)
      廣野誠子
    • Organizer
      第46回日本癌治療学会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-11-01
    • Related Report
      2009 Final Research Report
  • [Presentation] 網羅的遺伝子発現解析と免疫染色によるタンパク発現解析から同定した膵癌リンパ節転移予測マーカー2008

    • Author(s)
      廣野誠子
    • Organizer
      第17回日本がん転移学会
    • Place of Presentation
      鹿児島
    • Year and Date
      2008-07-24
    • Related Report
      2009 Final Research Report
  • [Presentation] 網羅的遺伝子発現解析とタンパク発現解析から同定した膵癌リンパ節転移予測マーカーの同定2008

    • Author(s)
      廣野誠子
    • Organizer
      第17回日本がん転移学会
    • Place of Presentation
      鹿児島
    • Year and Date
      2008-07-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] 網羅的遺伝子発現解析を用いた膵癌リンパ節転移予測マーカーの同定2008

    • Author(s)
      廣野誠子
    • Organizer
      第20回肝胆膵外科学会
    • Place of Presentation
      山形
    • Year and Date
      2008-05-30
    • Related Report
      2009 Final Research Report
  • [Presentation] 網羅的遺伝子発現システムを用いた膵癌リンパ節転移予測マーカーの同定2008

    • Author(s)
      廣野誠子
    • Organizer
      第20回日本肝胆膵外科学会
    • Place of Presentation
      山形
    • Year and Date
      2008-05-30
    • Related Report
      2008 Annual Research Report
  • [Presentation] 包括的遺伝子発現解析による膵癌リンパ節転移予測マーカーの同定2008

    • Author(s)
      廣野誠子
    • Organizer
      第108回日本外科学会
    • Place of Presentation
      長崎
    • Year and Date
      2008-05-15
    • Related Report
      2009 Final Research Report 2008 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi