Molecular diagnosis and carcinogenetic risk evaluation for lung cancer using methylation-specific DNA microarray

• Project/Area Number: 20790978
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Yamagata University
• Principal Investigator: ENDO Makoto Yamagata University, 医学部, 非常勤講師 (90420067)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: メチル化 / 肺癌 / メチル化診断チップ

Research Abstract

We devloped a fiber-type DNA microarray on which methylated sequence probes were mounted, and quantitatively analyzed DNA methylation rates (MRs) of 4 cancer-related genes of CpG island using it. In recurrent group rates of methylated RUNX3 was significantly higher than survivor. Analysis of DNA methylation status using DNA microarray could be a promising method for risk assessment in the recurrence of lung cancer.