Introduction of AAV9-D1NLS/CDK4 to failure heart to amplify matured myocardium
| Project/Area Number |
20790981
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
MIYAGI Naoto Tokyo Medical and Dental University, 医学部附属病院, 助教 (40463913)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 心臓大血管外科 / 遺伝子治療 / AAV9 / 心筋増殖誘導 / 拡張型心筋症 / 心筋梗塞 / 心筋細胞分裂誘導 / CDK4 / DlNLS / 重症心不全 |
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| Research Abstract |
This study was planned to introduce AAV9-D1NLS/CDK4 to healthy rat and hamster heart, rat infracted heart and DCM hamster to amplify matured myocardium. At the step of introduce D1NLS/CDK4 gene to AAV9 plasmid and after amplifying plasmid, expression of D1NLS/CDK4 gene expression was not detected. This result was because of size of D1NLS/CDK4 gene and it seemed to be difficult to introduce D1NLS/CDK4 gene to AAV9.
After the difficulty was revealed, the plasmid including D1NLS/CDK4 gene was injected to rat through rat tail vein (n=5), and expression of D1NLS/CDK4 gene was examined in rat heart, but the expression was not detected.
Now change of promoter has been tried to clear the mismatch between D1NLS/CDK4 gene and AAV9 vector.
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Report
(3 results)
Research Products
(1 results)
