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basic research for ischemia reperfusion injury following lung transplantation using a mouse orthotropic lung transplant model

Research Project

Project/Area Number 20790988
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionOkayama University

Principal Investigator

YAMANE Masaomi  Okayama University, 岡山大学病院, 助教 (20432643)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords肺移植 / Egr-1 / サイトカイン / 虚血再灌流障害 / マウス肺移植 / 虚血再還流障害
Research Abstract

Ischemia-reperfusion injury still remains main life-threatening complication following lung transplantation. It has been obscured that how warm ischemia affects on pulmonary graft injury as compared with classical cold preservation, when lungs are retrieved from non-heart beating donors. To clarify the differences between warm and cold preserved lungs in molecular mechanisms, PCR and western-blotting assays were performed to see the changes in expression levels of transcription factors, the downstream target genes, and phosphorylations of MAP kinases. We also employed Egr-1 knock out mouse to look at the effects of Egr-1 on graft functions and inflammatory gene expressions. When Egr-1 in the pulmonary grafts was deleted, lungs functioned obviously better than grafts from wild type mouse with Egr-1 expression. Up-regulation of inflammatory cytokines expression was not observed in Egr-1 KO grafts. These results suggest a novel possible approach to avoid pulmonary graft function from severe ischemia-reperfusion injury.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (5 results)

All 2009 2008

All Presentation (5 results)

  • [Presentation] 心停止ドナー肺移植後虚血再灌流障害からの回復における抗炎症性因子の重要性2009

    • Author(s)
      山本澄治、山根正修
    • Organizer
      第45回日本移植学会総会
    • Place of Presentation
      東京
    • Year and Date
      2009-09-17
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Egr-1ノックアウトマウスを用いた肺移植後再灌流障害2009

    • Author(s)
      山本澄治、山根正修
    • Organizer
      第45回日本移植学会総会
    • Place of Presentation
      東京
    • Year and Date
      2009-09-17
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] ラット心停止肺移植ドナー(NHBDs)肺モデルを用いた温虚血再灌流障害における分子レベルでの検討〜温虚血と冷虚血の違い2009

    • Author(s)
      山本澄治
    • Organizer
      日本肺および心肺移植研究会
    • Place of Presentation
      長崎
    • Year and Date
      2009-01-31
    • Related Report
      2008 Annual Research Report
  • [Presentation] ラット肺移植を用いたEGR1とサイトカインの発現と肺機能との関連における検討〜2008

    • Author(s)
      吉田修
    • Organizer
      第44回日本移植学会
    • Place of Presentation
      大阪
    • Year and Date
      2008-09-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] Early growth response (EGR)-1 and its target genes correlates with lung graft function in rats2008

    • Author(s)
      吉田修
    • Organizer
      The 22nd The transplantation Society
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      2008-08-12
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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