Therapeutic development of intervertebral disc degeneration targeting proteinase-activated receptor

• Project/Area Number: 20791030
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Mie University
• Principal Investigator: AKEDA Koji Mie University, 医学部・附属病院, 助教 (20422826)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 椎間板 / サイトカイン / 蛋白分解酵素 / 細胞外基質 / 椎間板変性

Research Abstract

The results of this study show that intervertebral disc (IVD) cells express PAR-2. The expression of PAR-2 is regulated by IL-1β stimulation. PAR-2 activation accelerates the expression of matrix degrading enzymes. PAR-2 may play an important role in the cytokine-mediated catabolic cascade and consequently may be involved in IVD degeneration.

Research Products

• [Journal Article] Expression of proteinase-activated receptor-2 in the intervertebral disc. (2009)
  • Author(s): Iida, R., Akeda, K., Kasai, Y., Masuda, K., Morimoto, R., Sakakibara, T., Sato, M., Uchida, A.
  • Journal Title: Spine (Phila Pa 1976) 34 Pages: 4-4
  • NAID: 10027239198
  • Peer Reviewed
• [Journal Article] Expression of proteinase-activated receptor-2 in the intervertebral disc (2009)
  • Author(s): Iida R, Akeda K, Kasai Y, Masuda K, Morimoto R, Sakakibara T, Sato M, Uchida A.
  • Journal Title: Spine (Phila Pa 1976) 34(5) Pages: 470-478
  • NAID: 10027239198
  • Peer Reviewed
• [Journal Article] Thoracic myelopathy with alkaptonuria (2008)
  • Author(s): Akeda, K., Kasai, Y., Kawakita, E., Matsumura, Y., Kono, T., Murata, T., Uchida, A.
  • Journal Title: Spine 33
  • Peer Reviewed
• [Journal Article] Tadpole system as new lumbar spinal instrumentation (2008)
  • Author(s): Kasai, Y., Inaba, T., Akeda, K., Uchida, A.
  • Journal Title: J Orthop Surg 3 Pages: 41-41
  • Peer Reviewed
• [Journal Article] Effect of osteogenic protein-1 on the matrix metabolism of boyine tendon cells (2008)
  • Author(s): Yamada, M., Akeda, K., Asanu ma, K., Thonar, E. J., An, H. S., Uchida, A., Masuda, K
  • Journal Title: J Orthop Res 26 Pages: 42-48
  • Peer Reviewed
• [Presentation] Function of the Tissue Renin-Angiotenensin System in Rat Intervertebal Disc Cells (2010)
  • Author(s): Morimoto R, Akeda K, et al
  • Organizer: 56th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans
• [Presentation] Expression of Bone Morphogenetic Proteins and their Receptors in the Normal and Following Transection (2010)
  • Author(s): Masaya T, Akeda K, et al
  • Organizer: 56th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans
• [Presentation] FUNCTIONAL ANALYSES OF PROTEINASE-ACTIVATED RECEPTOR 2 BY RAT INTERVERTEBRAL DISC CELLS (2009)
  • Author(s): Iida R, Akeda K, Kasai Y, Masuda K, Morimoto R, Iino T, Uchida A
  • Organizer: 55nd Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: Las Vegas
• [Presentation] FUNCTIONAL ANALYSES OF PROTEINASE-ACTIVATED RECEPTOR 2 BY RATINTERVERTEBRAL DISC CELLS (2009)
  • Author(s): ida R, Akeda K, Kasai Y, Masuda K, Morimoto R, Iino T, Uchida A
  • Organizer: 55nd Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: Las Vegas, NV
• [Presentation] 椎間板におけるProteinase-activated receptor 2の発現と機能 (2008)
  • Author(s): 飯田竜明田浩司、笠井裕一、舛田浩一、森本亮、内田淳正
  • Organizer: 第27回日本運動器移植・再生医学研究会
  • Place of Presentation: 岐阜市
  • Year and Date: 2008-09-27
• [Presentation] ラット椎間板細胞におけるproteinase-activated receptor-2の機能解析 (2008)
  • Author(s): 飯田竜、明田浩司、笠井裕一、舛田浩一、内田淳正
  • Organizer: 第23回日本整形外科基礎学術集会
  • Place of Presentation: 浜松市
• [Presentation] ヒト椎間板におけるProtease-Activated Receptor 2の発現-椎間板変性との関連性- (2008)
  • Author(s): 明田浩司、飯田竜、笠井裕一、舛田浩一、塩川靖夫、近藤哲士、榊原紀彦、佐藤昌良、内田淳正
  • Organizer: 第37回日本脊椎脊髄病学会
  • Place of Presentation: 東京
• [Presentation] Expression of Proteinase-Activated Receptor 2 on the Human Intervertebral Disc (2008)
  • Author(s): Ryu Iida, Koji Akeda, Yuichi Kasai, Koichi Masuda, Yasuo Shiokawa, Toshihiko Sakakibara, Masayoshi Sato, Takahiro Iino, Atsumasa Uchida
  • Organizer: 54nd Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: San Francisco
• [Presentation] EXPRESSION OF PROTEINASE-ACTIVATED RECEPTOR 2 ON THE HUMAN INTERVERTEBRAL DISC (2008)
  • Author(s): Ryu Iida; Koji Akeda; Yuichi Kasai; Koichi Masuda; Yasuo Shiokawa; Toshihiko Sakakibara; Masayoshi Sato; Takahiro Iino; Atsumasa Uchida
  • Organizer: The inaugural meeting of the World Forum for Spine Research
  • Place of Presentation: Kyoto