Analysis of metastatic factors of under coagulation disturbance

• Project/Area Number: 20791031
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Mie University
• Principal Investigator: KUNIHIRO Asanuma Mie University, 医学部・附属病院, 診療等従事者 (20378285)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: B16 melanoma / 肺転移 / 血液凝固 / 血液凝固抑制マウス / 高肺転移株 / 凝固因子 / 凝固抑制 / 癌転移 / 遺伝子発現

Research Abstract

Lung metastases was decreased depend on the inhibition of coagulation activity. Highly lung metastatic cell lines Wa9 and Co9 was successfully made using coagulation inhibited mice and wild mice, respectively. Analysis of expressed genes between Wild, Wa9 and Co9 by DNA array is currently in progress.

Research Products

• [Journal Article] Effect of small interference RNA (siRNA) for ADAMTS5 on intervertebral disc degeneration in the rabbit anular needle puncture model. (2009)
  • Author(s): Seki S. Asanuma-Abe Y, Masuda K, Kawaguchi Y, Asanuma K, Muehleman C, Iwai A, Kimura T.
  • Journal Title: Arthritis Res Ther. 11(6)
  • Peer Reviewed
• [Journal Article] Spinal neurocutaneous melanosis without cutaneous nevi. (2008)
  • Author(s): Asanuma K, Kasai Y, Takegami K, Ito H, Yoshikawa T, Uchida A.
  • Journal Title: Spine 1 33(2)
  • Peer Reviewed
• [Journal Article] Effect of osteogenic protein-1 on the matrix metaboplism of bovine tendon cells. (2008)
  • Author(s): Yamada M, Akeda K, Asanuma K, Thonar EJ, An HS, Uchida A, Masuda K.
  • Journal Title: J Orthop Res. 26(1) Pages: 42-8
  • Peer Reviewed
• [Presentation] The inhibition of coagulation activity by warfarin down-regulates lung metastasis (2009)
  • Author(s): Kunihiro Asanuma
  • Organizer: 55^<th> annual meeting of the Orthopaedic Research Society
  • Place of Presentation: Las Vegasu
  • Year and Date: 2009-02-23
• [Presentation] Thrombin阻害薬‘argatroban'による関節炎の抑制効果の解析 (2009)
  • Author(s): 淺沼邦洋、吉田格之進、吉川智朗、林辰弥、秋田展幸、岡本貴行、長谷川正裕、須藤啓広、鈴木宏治、内田淳正
  • Organizer: 第24回日本整形外科学会基礎学術集会
  • Place of Presentation: 横浜市
• [Presentation] The inhibition of coagulation activity by warfarin down-regulates lung metastasis. (2009)
  • Author(s): Kunihiro Asanuma, Nobuyuki Akita, Tatsuya Hayashi, Kakunoshin Yoshida, Takayuki Okamoto, Akihiko Matsumine, Koji Suzuki, Atsumasa Uchida
  • Organizer: 55th Orthopedic Research society
  • Place of Presentation: Las Vegas Las Vegas, Nevada USA
• [Presentation] トロンビンの椎間板注入による椎間板変性モデル (2008)
  • Author(s): 淺沼邦洋、阿部由美子、Rajeawari Pichika, Carol Muehleman、角谷賢一朗、Haward An、内田淳正、舛田浩一
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都市