The analysis of intervertebral disc degeneration mechanism

• Project/Area Number: 20791035
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Kobe University
• Principal Investigator: MAENO Koichiro Kobe University, 医学部附属病院, 助教 (70403269)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 遺伝子 / シグナル伝達 / 再生医学 / 椎間板変性 / ヒト椎間板不死化細胞株 / Fas / FasL / RNA干渉 / 過剰発現

Research Abstract

It has been proved that the proinflammatory cytokine IL-1β increased in Fas-ligand overexpressed intervertebral nucleus pulposus cells. It was suggested that Fas-ligand greatly take part in the inducement of the proinflammatory cytokine. On the other hand, we were not able to prove that Fas-ligand take part in the cellular apoptosis.