Identification of BMP responsive osteoprogenitor cells in muscle tissue

• Project/Area Number: 20791050
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Saitama Medical University
• Principal Investigator: FUKUDA Toru Saitama Medical University, 医学部, 助教 (20301492)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: BMP / 骨形成 / トランスジェニック

Research Abstract

BMPs were identified as factors responsible for induction of bone formation. However, the BMP responsive osteoprogenitor cells were remains unknown. To identify these progenitors, we attempted to generate a ALK2(R206H) transgenic mice.

Research Products

• [Journal Article] Protein Phosphatase Magnesium-Dependent 1A-Mediated Inhibition of BMP Signaling is Independent of Smad Dephosphorylation (2010)
  • Author(s): Kokabu, S
  • Journal Title: Journal of Bone and Mineral Research
  • Peer Reviewed
• [Journal Article] Constitutively activated ALK2 and increased SMAD1/5 cooperatively induce bone morphogenetic protein signaling in fibrodysplasia ossificans progressiva (2009)
  • Author(s): Fukuda T, Kohda M, Kanomata K, Nojima J, Nakamura A, Kamizono J, Noguchi Y, Iwakiri K, Kondo T, Kurose J, Endo K, Awakura T, Fukushi J, Nakashima Y, Chiyonobu T, Kawara A, Nishida Y, Wada I, Akita M, Komori T, Nakayama K, Nanba A, Maruki Y, Yoda T, Tomoda H, Yu PB, Shore EM, Kaplan FS, Miyazono K, Matsuoka M, Ikebuchi K, Ohtake A, Oda H, Jimi E, Owan I, Okazaki Y, Katagiri T.
  • Journal Title: J. Biol. Chem. 284 Pages: 7149-7156
  • Peer Reviewed
• [Journal Article] Protein Phosphatase Magnesium-Dependent 1A-Mediated Inhibition of BMP Signaling is Independent of Smad Dephosphorylation (2009)
  • Author(s): Kokabu S, Nojima J, Kanomata K, Ohte S, Yoda T, Fukuda T, Katagiri T
  • Journal Title: J. Bone Miner. Res. (in press)
  • Peer Reviewed
• [Journal Article] miR-210 promotes osteoblastic differentiation through inhibition of AcvR1b (2009)
  • Author(s): Mizuno Y, Tokuzawa Y, Ninomiya Y, Yagi K, Yatsuka-Kanesaki Y, Suda T, Fukuda T, Katagiri T, Kondoh Y, Amemiya T, Tashiro H, Okazaki Y
  • Journal Title: FEBS Lett. 583 Pages: 2263-226
  • Peer Reviewed
• [Journal Article] Distinct function of 2 chromatin remodeling complexes that share a common subunit, Williams syndrome transcription factor (WSTF) (2009)
  • Author(s): Yoshimura K, Kitagawa H, Fujiki R, Tanabe M, Takezawa S, Takada I, Yamaoka I, Yonezawa M, Kondo T, Furutani Y, Yagi H, Yoshinaga S, Masuda T, Fukuda T, Yamamoto Y, Ebihara K, Li DY, Matsuoka R, Takeuchi JK, Matsumoto T, Kato S
  • Journal Title: Proc. Natl. Acad. Sci. U S A. 106 Pages: 9280-9285
  • Peer Reviewed
• [Journal Article] DRAGON, a GPI-anchored membrane protein, inhibits BMP signaling in C2C12 myoblasts (2009)
  • Author(s): Kanomata K, Kokabu S, Nojima J, Fukuda T, Katagiri T
  • Journal Title: Genes Cells 14 Pages: 695-702
  • Peer Reviewed
• [Journal Article] DRAGON, a GPI-anchored membrane protein, inhibits BMP signaling in C2C12 myoblasts. (2009)
  • Author(s): Kanomata, K
  • Journal Title: Genes to Cells 14 Pages: 695-702
  • Peer Reviewed
• [Journal Article] Distinct function of 2 chromatin remodeling complexes that Share a common subunit, Williams syndrome transcription factor (WSTF). (2009)
  • Author(s): Yoshimura, K
  • Journal Title: Proceedings of the National Academy of Sciences 106 Pages: 9280-9285
  • Peer Reviewed
• [Journal Article] miR-210 promotes osteoblastic differentiation through inhibition of AcvR1b. (2009)
  • Author(s): Mizuno, Y
  • Journal Title: FEBS Letters 583 Pages: 2263-2268
  • Peer Reviewed
• [Journal Article] Constitutively activated ALK2 and increased SMAD1/5 cooperatively induce bone morphogenetic protein signaling in fibrodysplasia ossificans progressiva (2009)
  • Author(s): Fukuda T
  • Journal Title: The Journal of Biological Chemistory 284 Pages: 7149-7156
  • Peer Reviewed
• [Journal Article] A unique mutation of ALK2, G356D, found in a patient with fibrodysplasia ossificans progressiva is a moderately activated BMP type I receptor (2008)
  • Author(s): Fukuda T, Kanomata K, Nojima J, Kokabu S, Akita M, Ikebuchi K, Jimi E, Komori T, Maruki Y, Matsuoka M, Miyazono K, Nakayama K, Nanba A, Tomoda H, Okazaki Y, Ohtake A, Oda H, Owan I, Yoda T, Haga N, Furuya H, Katagiri T
  • Journal Title: Biochem. Biophys. Res. Commun. 377 Pages: 905-909
  • Peer Reviewed
• [Journal Article] miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation (2008)
  • Author(s): Mizuno Y, Yagi K, Tokuzawa Y, Kanesaki-Yatsuka Y, Suda T, Katagiri T, Fukuda T, Maruyama M, Okuda A, Amemiya T, Kondoh Y, Tashiro H, Okazaki Y
  • Journal Title: Biochem. Biophys. Res. Commun. 368 Pages: 267-272
  • Peer Reviewed
• [Journal Article] A unique mutation of ALK2, G356D, found in a patient with fibrodysplasia ossificans progressiva is a moderately activated BMP type I receptor (2008)
  • Author(s): Fukuda T
  • Journal Title: Biochemical and Biophysical Research Communications 377 Pages: 905-909
  • Peer Reviewed
• [Presentation] WntとBMPリガンドによる相乗的な骨芽細胞の誘導機構 (2009)
  • Author(s): 福田亨
  • Organizer: 第32回BMP日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-12
• [Presentation] Genome network analysis reveal Id4 as a molecular switch promoting mouse osteoblast differentiation (2009)
  • Author(s): 徳澤佳美
  • Organizer: 第32回BMP日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-12
• [Presentation] Crosstalk of BMP signaling and Runx2 in osteoblastic differentiation of C2C12 myoblasts (2009)
  • Author(s): 大手聡
  • Organizer: 第32回BMP日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-10
• [Presentation] miR-210 promotes osteoblastic differentiation through inhibition of ACVR1b (2009)
  • Author(s): 水野洋介
  • Organizer: 第32回BMP日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-09
• [Presentation] Roles of phosphorylation and dephosphorylation of Smad1 on BMP-induced osteoblast differentiation of C2C12 myoblasts (2009)
  • Author(s): 古株彰一郎
  • Organizer: 第32回BMP日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-09
• [Presentation] 筋組織内に異所性骨化を生じる遺伝性難病Fibrodysplasia Ossificans Progressiva (FOP) (2009)
  • Author(s): 福田亨
  • Organizer: 第10回運動器科学研究会
  • Place of Presentation: 東京ステーションコンファレンス(東京都)
  • Year and Date: 2009-09-18
• [Presentation] 筋組織内に異所性骨化を生じる遺伝性難Fibrodysplasia Ossificans Progressiva (FOP) (2009)
  • Author(s): 福田亨
  • Organizer: 第10回運動器科学研究会
  • Place of Presentation: 東京ステーションコンファレンス(東京都)
  • Year and Date: 2009-09-18
• [Presentation] PPM1A and SCP1 suppress BMP activity via a novel mechanism independent of Smad C-terminal dephosphorylation (2009)
  • Author(s): 古株彰一郎
  • Organizer: 31^<st> ASBMR (American Society for Bone and Mineral Research) annual meeting
  • Place of Presentation: コロラドコンベンションセンター(アメリカ)
  • Year and Date: 2009-09-13
• [Presentation] Functional Analysis of Mutant ALK2 Receptors Found in Fibrodysplasia ossificans progressiva (FOP) (2009)
  • Author(s): 福田亨
  • Organizer: 31st ASBMR (American Society for Bone and Mineral Research) annual meeting
  • Place of Presentation: コロラドコンベンションセンター(アメリカ
  • Year and Date: 2009-09-12
• [Presentation] Functional Analysis of Mutant ALK2 Receptors Found in Fibrodysplasia ossificans progressiva (FOP) (2009)
  • Author(s): 福田亨
  • Organizer: 31^<st> ASBMR (American Society for Bone and Mineral Research) annual meeting
  • Place of Presentation: コロラドコンベンションセンター(アメリカ)
  • Year and Date: 2009-09-12
• [Presentation] Fibrodysplasia ossificans progressiva (FOP)で新たに同定されたALK2変異体の機能解析 (2009)
  • Author(s): 福田亨
  • Organizer: 第16回BMP研究会
  • Place of Presentation: 大阪大学中之島センター(大阪府)
  • Year and Date: 2009-07-26
• [Presentation] Smadlのリン酸化・脱リン酸化による骨芽細胞分化誘導の制御 (2009)
  • Author(s): 古株彰一郎
  • Organizer: 第16回BMP研究会
  • Place of Presentation: 大阪大学中之島センター(大阪府)
  • Year and Date: 2009-07-26
• [Presentation] Fibrodysplasia ossificans progressiva (FOP)で新たに同定されたALK2変異体の機能解析 (2009)
  • Author(s): 福田亨
  • Organizer: 第16回BMP研究会一
  • Place of Presentation: 大阪大学中之島センター(大阪府)
  • Year and Date: 2009-07-26
• [Presentation] Fibrodysplasia ossificans progressiva (FOP)で同定されたALK2変異体の解析 (2009)
  • Author(s): 福田亨
  • Organizer: 第27回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2009-07-25
• [Presentation] Smad C末端のホスファターゼPPM1AとSCP1はリンカー領域のMAPKリン酸化部位を介してBMPシグナルを抑制する (2009)
  • Author(s): 古株彰一郎
  • Organizer: 第27回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2009-07-25