A role of ectodomain shedding of RANK in osteoclastogenesis

• Project/Area Number: 20791051
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Keio University
• Principal Investigator: HAKOZAKI Akihiro Keio University, 医学部, 研究員(非常勤) (10365299)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 破骨細胞 / receptor activator of NF-κB (RANK) / TACE / ADAM17 / ectodomain shedding / receptor activator of NF-KB (RANK) / recetor activator of NF-κB(RANK)

Research Abstract

We showed that receptor activator of NF-kB (RANK), one of the most essential molecules in osteoclastogenesis, is proteolytically cleaved and released from the cell surface. Furthermore, we found that that the cleavage of RANK is induced by binding to its cognitive ligand, RANKL, and that the ectodomain shedding of RANK potentially functions as a negative regulatory mechanism in osteoclastogenesis.

Research Products

• [Journal Article] Receptor Activator of NF-κB Ligand Induces Ectodomain Shedding of Receptor Activator of NF-κB in Murine RAW264. 7 Macrophages (2010)
  • Author(s): Hakozaki A, Yoda M, Tohmonda T, Furukawa M, Hikata T, Uchikawa S, Takaishi H, Matsumoto M, Chiba K, Horiuchi K, Toyama Y.
  • Journal Title: The Journal of Immunology 184(5) Pages: 2442-2448
  • Peer Reviewed
• [Journal Article] Receptor Activator of NF-KB Ligand Induces Ectodomain Shedding of Receptor Activator of NF-KB in Murine RAW264.7 Macrophages (2010)
  • Author(s): Akihiro Hakozaki
  • Journal Title: Journal of Immunology 184(5) Pages: 2442-2448
  • Peer Reviewed
• [Presentation] TNFa converting enzyme regulates proteolytic cleavage of receptor activator of NFk-B (2009)
  • Author(s): Akihiro Hakozaki
  • Organizer: American Society for Bone and Mineral Research 2009 Annual Meeting
  • Place of Presentation: Colorado Convention Center Denver.
  • Year and Date: 2009-09-12
• [Presentation] RAW264.7細胞においてRANKL刺激はRANK sheddingを誘導する (2009)
  • Author(s): 箱崎彰裕
  • Organizer: 第27回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-07-23
• [Presentation] TNFαconverting enzyme regulates proteolytic cleavage of receptor activator of NFκ-B (2009)
  • Author(s): Akihiro Hakozaki, Keisuke Horiuchi, Tomohiro Hikata, Mitsuru Furukawa, Shinichi Uchikawa, Tomoaki Mori, Masaki Yoda, Takahide Tohmonda, Morio Matsumoto, Kazuhiro Chiba, Hironari Takaishi, Yoshiaki Toyama
  • Organizer: ASBMR 2009 Annual Meeting
  • Place of Presentation: Colorado Convention Center Denver
• [Presentation] RAW264. 7細胞においてRANKL刺激はRANK sheddingを誘導する (2009)
  • Author(s): 箱崎彰裕、堀内圭輔、依田昌樹、東門田誠一、日方智宏、内川伸一、古川満、松本守雄、高石官成、戸山芳昭
  • Organizer: 第27回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場
• [Presentation] RANKはADAMI7によりsheddingを受け可溶型となる (2008)
  • Author(s): 箱崎彰裕
  • Organizer: 第26回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2008-10-29
• [Presentation] RANKはADAM17によりsheddingを受け可溶型となる (2008)
  • Author(s): 箱崎彰裕、堀内圭輔、依田昌樹、東門田誠一、日方智宏、内川伸一、古川満、松本守雄、高石官成、戸山芳昭
  • Organizer: 第26回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場