| Project/Area Number |
20791052
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Keio University |
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Principal Investigator |
TSUJI Osahiko Keio University, 医学部, 助教 (70424166)
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| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 脊椎損傷 / iPS細胞 / 神経幹細胞 / 脊髄損傷 |
|---|
| Research Abstract |
Here we demonstrated the directed neural differentiation of murine iPS cells and examined their therapeutic potential in a mouse spinal cord injury (SCI) model. 'Safe' iPS-derived neurospheres, which had been pre-evaluated as non-tumorigenic by their transplantation into NOD/SCID mouse brain, produced neurons, astrocytes, and oligodendrocytes in vitro. Furthermore, when the 'safe' iPS-derived neurospheres were transplanted into the spinal cord 9 days after contusive injury, they differentiated into all three neural lineages without forming teratomas or other tumors. They also participated in re-myelination and induced the axonal re-growth of host 5HT+ serotonergic fibers, promoting locomotor function recovery. These results suggest that pre-evaluated safe' PS clone-derived neural stem/progenitor cells may be a promising cell source for transplantation therapy for SCI.
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