Cholinergic regulation of nociception in dorsal root ganglion

• Project/Area Number: 20791068
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Shiga University of Medical Science
• Principal Investigator: J・P Bellier Shiga University of Medical Science, 分子神経科学研究センター, 助教 (80346022)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: アセチルコリン / acetylcholine / 後根神経節 / dorsal root ganglion / pChAT / pain / 神経障害痛モデルラット / rat neuropathic pain model / 神経障害痛のラットモデル / neuropathic pain model

Research Abstract

The presence of a splice variant of the enzyme choline acetyltransferase (pChAT) has been demonstrated in the dorsal root ganglion neurons. The role of acetylcholine synthesized by pChAT in DRG has been investigated in rat model of neuropathic pain with the use of agonist of cholinergic system. Ours experimental results suggest that a cholinergic regulation of nociception take place at the level of primary sensory neurons. Moreover cholinergic marker was investigated in human DRG. Our results suggest that pChAT might be a relevant target for analgesia in human.

Research Products

• [Journal Article] Axotomy alters alternative splicing of choline acetyltransferase in the rat dorsal motor nucleus of the vagus nerve (2009)
  • Author(s): Saito A, Sato T, Okano H, Toyoda K, Bamba H, Kimura S, Bellier JP, Matsuo A, Kimura H, Hisa Y, Tooyama I
  • Journal Title: Journal of Comparative Neurology 513 Pages: 237-248
  • Peer Reviewed
• [Journal Article] Axotomy alters alternative splicing of choline acetyltransferase in the rat dorsal motor nucleus of the vagus nerve (2009)
  • Author(s): Saito A, Sato T, Okano H, Toyoda K, Bamba H, Kimura S, Bellier JP, Matsuo A, Kimura H, Hisa Y, Tooyama I
  • Journal Title: Journal of Comparative Neurology 513(2) Pages: 237-48
  • Peer Reviewed
• [Journal Article] Distribution of natriuretic peptide receptor-C immunoreactivity in the rat brainstem and its relationship to cholinergic and catecholaminergic neurons (2008)
  • Author(s): Abdelalim EM, Masuda C, Bellier JP, Saito A, Yamamoto S, Mori N, Tooyama I
  • Journal Title: Neuroscience 155 Pages: 192-202
  • Peer Reviewed
• [Journal Article] First visualization of cholinergic cells and fibers by immunohistochemistry for choline acetyltransferase of the common type in the optic lobe and peduncle complex of Octopus vulgaris (2008)
  • Author(s): D'Este L, Kimura S, Casini A, Matsuo A, Bellier JP, Kimura H, Renda TG
  • Journal Title: Journal of Comparative Neurology 509 Pages: 566-579
  • Peer Reviewed
• [Journal Article] First visualization of cholinergic cells and fibers by immunohistochemistry for choline acetyltransferase of the common type in the optic lobe and peduncle complex of Octopus vulgaris (2008)
  • Author(s): D'Este L, Kimura S, Casini A, Matsuo A, Bellier JP, Kimura H , Renda TG
  • Journal Title: Journal of Comparative Neurology 509(6) Pages: 566-79
  • Peer Reviewed
• [Presentation] Immunohistochemical localization of the peripheral type choline acetyltransferase in colon and dorsal root ganglion of human. (2009)
  • Author(s): J. P Bellier, S Kimura, Y Sakaue, H Kimura.
  • Organizer: 第50回日本組織細胞化学会総会・学術集会事務局
  • Place of Presentation: ピアザ淡海滋賀県立県民交流センター
• [Presentation] Immunolocalization of choline acetyltransferase of common type (cChat) in the nervous system of Octopus vulgaris. (2009)
  • Author(s): A Casini, L D'Este, S Kimura, J. P Bellier, H Kimura, T. G Renda.
  • Organizer: XXXIII Congress of the Italian Society of Histochemistry.
  • Place of Presentation: Roma Italia.