| Project/Area Number |
20791070
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Osaka University |
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Principal Investigator |
NAKAE Aya Osaka University, 医学系研究科, 特任准教授(常勤) (60379170)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | snoRNA / RNA編集 / 疼痛モデル / 核小体低分子RNA / セロトニン2C受容体 / 眼窩下神経絞扼モデル / 選択的スプライシング / RBII-52 / プラダー・ウィリ症候群 / allodynia / 核小体RNA |
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| Research Abstract |
The serotonin 2C receptor (5HTR2C) receives RNA editing. It is also known that as a result of alternative splicing of the 5HTR2C, a flame shift occurs and a non-functional receptor is created. Having studied the 5HTR2C RNA editing efficiency in a rat oro-facial neuropathic pain model (Nakae et al. EJN 2008), our interest turned to the factor contributing to RNA editing and efficacy of alternative splicing. In human study, HBII-52 (one kind of small nuclear RNA ; equal in function to RBII-52) contributes to regulating the 5HTR2C RNA editing and alternative splicing. In this research, an infra-orbital loose ligation model was used. The tendency of RBII-52 expression in injured and sham operated animals decreased. On the other hand, only the Va containing pattern (non-functional) increased in injured and sham rats. The relationship between RBII-52 expression, the 5HT2C-R alternative splicing and RNA editing was unclear. Modulation of 5HTR2C activity might be capable of far finer tuning than we previously imagined through noxious stimuli response, such as neuropathic pain.
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