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Temperature-dependent response in neuronal injury by hypoxia-induced microglia

Research Project

Project/Area Number 20791077
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Anesthesiology/Resuscitation studies
Research InstitutionYamaguchi University

Principal Investigator

MATSUI Tomohiro  Yamaguchi University, 大学院・医学系研究科, 助教 (50314828)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords脳低温療法 / マイクログリア / ATP / TNF-α / IL-6 / NO / p38MAPK / 高温培養 / p38 MAPK / 一酸化窒素(NO) / インターロイキン(IL)-6 / アデノシン三リン酸(ATP) / 炎症
Research Abstract

Pro-inflammatory cytokines and NO are considered responsible for exacerbating brain injury. Activated microglia produce these potentially cytotoxic factors during neuron destruction. Therefore, the beneficial effects of therapeutic hypothermia on neuroprotection are considered to be due, in part, to suppression of post-injury inflammatory factors by microglia. In this study, I examined whether altering culture temperature modifies production of cytokines and NO by microglia activated with ATP, a product leaked from injured neurons/cells after brain insults. Compared to normothermia, hypothermia ecreased ATP-induced production of pro-inflammatory cytokines, TNF- α and IL-6, within 6 h of culture. NO production was reduced by hypothermia but augmented by hyperthermia at 6 h. In addition, activation of p38 MAPK was reduced in hypothermia at 1 min, compared to normothermia. In conclusion, hypothermia reduced the rapid activation of p38 MAPK and the following TNF-α, IL-6, and NO production in ATP-activated microglia, suggesting that the reduction of the early-phase inflammatory factors via suppression of p38 MAPK in microglia is one possible neuroprotective mechanism of therapeutic hypothermia. Hyperthermia specifically increased the early-phase NO production in these cells. These temperature-dependent changes in NO production may imply that NO in the early phase is useful as an important clinical marker in hypothermia-related neuronal protection and in hyperthermia-related neuronal injury.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (15 results)

All 2010 2009 2008

All Journal Article (8 results) (of which Peer Reviewed: 4 results) Presentation (4 results) Book (3 results)

  • [Journal Article] 低温・高温下におけるマイクログリアのサイトカインおよびNO産生動態2010

    • Author(s)
      松井智浩
    • Journal Title

      山口医学 59

      Pages: 17-21

    • NAID

      10025981650

    • Related Report
      2009 Final Research Report
  • [Journal Article] 低温・高温下におけるマイクログリアのサイトカインおよびNO産生動態2010

    • Author(s)
      松井智浩
    • Journal Title

      山口医学 58

      Pages: 17-21

    • NAID

      10025981650

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Hypothermia reduces the release of inflammatory factors via suppression of p38 MAPK in ATP-activated rat microglia2009

    • Author(s)
      MATSUI T
    • Journal Title

      ニュー・フロンティア・プロジェクト報告集 10

      Pages: 54-58

    • Related Report
      2009 Final Research Report
  • [Journal Article] Hypothermia reduces the release of inHammatory factors via suppression of p38 MAPK in ATP-activated rat microglia2009

    • Author(s)
      Tomohiro MATSUI
    • Journal Title

      ニュー・フロンティア・プロジェクト報告集 10

      Pages: 54-58

    • Related Report
      2009 Annual Research Report
  • [Journal Article] IL-10 production is reduced by hypothermia but augmented by hyperthermia in rat microglia2008

    • Author(s)
      Matsui T, Kakeda T
    • Journal Title

      J Neurotrauma 25

      Pages: 709-715

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The evidence for sweet substanceinduced analgesia in adult human2008

    • Author(s)
      Kakeda T, Ito M, Matsui T, Ishikawa T
    • Journal Title

      Pain Research 23

      Pages: 159-166

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] IL-10 Production Is Reduced by Hypothermia but Augmented by Hyperthermia in Rat Microglia2008

    • Author(s)
      TOMOHIRO MATSUI, et.al.
    • Journal Title

      JOURNAL OF NEUROTRAUMA 25

      Pages: 709-715

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Evidence for Sweet Substance-induced Analgesia in Adult Human2008

    • Author(s)
      TAKAHIRO KAKEDA, et.al.
    • Journal Title

      PAIN RESEARCH 23

      Pages: 161-168

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 低温・高温下におけるマイクログリアのサイトカインおよびNO産生動態(山口大学医学会中村賞受賞者講演)2010

    • Author(s)
      松井智浩
    • Organizer
      第113回山口大学医学会
    • Place of Presentation
      宇部(山口県)
    • Year and Date
      2010-02-20
    • Related Report
      2009 Final Research Report
  • [Presentation] 低温・高温下におけるマイクログリアのサイトカインおよびNO産生動態2010

    • Author(s)
      松井智浩
    • Organizer
      第113回山口大学医学会
    • Place of Presentation
      宇部(山口県)
    • Year and Date
      2010-02-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] Inflammatory factors produced by ATP-activated microglia are reduced by hypothermia2008

    • Author(s)
      Matsui T
    • Organizer
      The 26th Annual National Neurotrauma Society Symposium
    • Place of Presentation
      Orlando (FL, USA)
    • Year and Date
      2008-07-29
    • Related Report
      2009 Final Research Report
  • [Presentation] INFLAMMATORY FACTORS PRODUCED BY ATP-ACTIVATED MICROGLIAARE REDUCED BY HYPOTHERMIA2008

    • Author(s)
      TOMOHIRO MATSUI, et.al.
    • Organizer
      The 26th Annual National Neurotrauma Society Symposium
    • Place of Presentation
      Orlando, FL, USA
    • Year and Date
      2008-07-29
    • Related Report
      2008 Annual Research Report
  • [Book] 臨床検査学講座/免疫検査学第2版2010

    • Author(s)
      松井智浩, ほか
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2009 Final Research Report
  • [Book] 臨床検査学講座/免疫検査学第2版「赤血球抗体検査」2010

    • Author(s)
      松井智浩, ほか
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2009 Annual Research Report
  • [Book] 臨床検査学講座免疫検査学2008

    • Author(s)
      窪田哲朗ほか
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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