The influences of the anesthetics on inhibitory interneurons and dopamine release in reward neural circuit in rats.

• Project/Area Number: 20791087
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Yokohama City University
• Principal Investigator: ISHIWA Dai Yokohama City University, 医学研究科, 客員研究員 (30457858)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 麻酔学 / 報酬系神経回路 / 介在ニューロン / ドパミン放出 / 神経可塑性

Research Abstract

To clarify the cellular mechanisms of addiction with anesthetics, I performed extracellular field potential recording on rat VTA slices. I found that subclinical concentration of propofol (0.5μM) potentiated the field potential in VTA about 25 % of control value even though much higher concentration of propofol (5-50μM) suppressed it dose dependently. I could not evaluate influences of propofol in dopamine release in the VTA with in vitro setting.