| Project/Area Number |
20791121
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
|
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | インターロイキン-15 / 遺伝子治療 / 免疫治療 / 筋層非浸潤性膀胱癌 / 非筋層浸潤性膀胱癌 |
|---|
| Research Abstract |
In proportion to the liposomal transfection efficiency in vitro, the IL-15 concentrations in the culture supernatant were dose-dependent after the IL-15 gene plasmid transfection. Our results in vivo showed that using liposome-mediated in situ gene transfer via simple intravesical instillation, intravesical IL-15 plasmid transfection in situ exhibits significant tumor suppression in an orthotopic bladder cancer model, and that CD8^+ T-cells seemed to be associated with its anti-tumor effect. In mice that survived the intravesical IL-15 gene therapy, rechallenged MBT-2 cells were suppressed and they had the potential to acquire immunologic memory. In conclusion, the present data indicate that IL-15 gene therapy may be a promising new adjuvant therapy for non-muscle invasive bladder cancer and a useful substitute for BCG treatment.
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