Analysis of the autoantigens and its localization in murine experimental autoimmune orchitis

• Project/Area Number: 20791123
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Tokyo Medical University
• Principal Investigator: NAITO Munekazu Tokyo Medical University, 医学部, 講師 (10384984)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 精巣 / 精子形成 / 血液精巣関門 / 自己免疫 / 炎症 / 精巣血液関門 / マウス / タンパク質 / 抗原

Research Abstract

The testis is known as an immunologically privileged organ. In particular, the blood-testis-barrier (BTB) formed by Sertoli cells protects autoimmunogeneic spermatids from attack by the self immune system. In our present study, we showed the BTB at the tubuli recti and the rete testis is incomplete. Moreover, 18 genes were found from the result of screening used by murine autoimmune orchits model.

Research Products

• [Journal Article] Difference in abundance of blood and lymphatic capillaries in the murine epididymis (2010)
  • Author(s): Hirai S, Naito M, Terayama H, Ning Q, Miura M, Itoh M
  • Journal Title: Medical Molecular Morphology 43 Pages: 37-42
  • NAID: 10026443189
  • Peer Reviewed
• [Journal Article] Cytoprotective effect of imatinib mesylate in non-BCR-ABL-expressing cells along with autophagosome formation. (2010)
  • Author(s): Ohtomo
  • Journal Title: Biochemical and Biophysical Research Communications 391 Pages: 310-315
  • Peer Reviewed
• [Journal Article] Effects of splenectomy on spontaneously chronic pancreatitis in aly/aly mice. (2010)
  • Author(s): Wang H
  • Journal Title: Clinical and Developmental Immunology (In press)
  • Peer Reviewed
• [Journal Article] Multilayered structure of the basal lamina of the tubuli recti in normal mice. (2010)
  • Author(s): Tainosho S
  • Journal Title: Medical Molecular Morphology (In press)
  • Peer Reviewed
• [Journal Article] Difference in abundance of blood and lymphatic capillaries in the murine epididymis (2010)
  • Author(s): Hirai S
  • Journal Title: Medical Molecular Morphology (In press)
  • Peer Reviewed
• [Journal Article] Histopathology of the tubuli recti at the start of experimental autoimmune orchitis in mice (2009)
  • Author(s): Naito M, Hirai S, Terayama H, Ning Q, Kawata S, Itoh M
  • Journal Title: Medical Molecular Morphology 42 Pages: 230-235
  • NAID: 10027269605
  • Peer Reviewed
• [Journal Article] Effect of unilateral testicular rupture on histopathology and germ cell delayed-type hypersensitivity in C3H/He and A/J mice (2009)
  • Author(s): Naito M, Sakamoto Y, Terayama H, Hirai S, Ning Q, Aota Y, Itoh M
  • Journal Title: Journal of Reproductive Immunology 81 Pages: 55-61
  • Peer Reviewed
• [Journal Article] Growth inhibitory effects of vitamin K2 on colon cancer cell lines via different types of cell death including autophagy and apoptosis (2009)
  • Author(s): Kawakita H, Tsuchida A, Miyazawa K, Naito M, Shigoka M, Kyo B, Enomoto M, Wada T, Katsumata K, Ohyashiki K, Itoh M, Tomoda A, Aoki T
  • Journal Title: International Journal of Molecular Medicine 23 Pages: 709-716
  • Peer Reviewed
• [Journal Article] Histopathology of the tubuli recti at the start of experimental autoimmune orchitis in mice. (2009)
  • Author(s): Naito M
  • Journal Title: Medical Molecular Morphology 42 Pages: 230-235
  • NAID: 10027269605
  • Peer Reviewed
• [Journal Article] Growth inhibitory effects of vitamin K2 on colon cancer cel lines via different types of cell death including autophagy and apoptosis. (2009)
  • Author(s): Kawakita H
  • Journal Title: International Journal of Molecular Medicine 23 Pages: 709-716
  • Peer Reviewed
• [Journal Article] Histopathology and delayed-type hypersensitivity of C3H/He and A/J mice after unilateral testicular rupture (2009)
  • Author(s): Naito M
  • Journal Title: Journal of Reproductive Immunology (In press)
  • Peer Reviewed
• [Journal Article] The presence of intra-tubular lymphocytes in normal testis of the mouse (2008)
  • Author(s): Naito M, Terayama H, Hirai S, Qu N, Moriyama H, Itoh M
  • Journal Title: Okajimas Folia Anatomica Japonica 85 Pages: 91-96
  • NAID: 130004495055
  • Peer Reviewed
• [Journal Article] Patterns of Infiltration of Lymphocytes into the Testis Under Normal and Pathological Conditions in Mice (2008)
  • Author(s): Naito M, Itoh M
  • Journal Title: American Journal of Reproductive Immunology 59(Review) Pages: 55-61
  • Peer Reviewed
• [Journal Article] Caput epididymitis but not orchitis was induced by vasectomy in a murine model of experimental autoimmune orchitis (2008)
  • Author(s): Qu N, Terayama H, Naito M, Ogawa Y, Hirai S, Kitaoka M, Yi SQ, Itoh M
  • Journal Title: Reproduction 135 Pages: 859-866
  • Peer Reviewed
• [Journal Article] Vitamin K2 induces autophagy and apoptosis simultaneously in leukemia cells (2008)
  • Author(s): Yokoyama T, Miyazawa K, Naito M, Toyotake J, Tauchi T, Itoh M, Yuo A, Hayashi Y, Georgescu MM, Kondo Y, Kondo S, Ohyashiki K.
  • Journal Title: Autophagy 4 Pages: 629-640
  • Peer Reviewed
• [Journal Article] Absence of the celiac trunk: Case report and review of the literature (2008)
  • Author(s): Shuang-Qin Yi, Terayama H, Naito M, Hirai S, Alimujang S, Yi N, Tanaka S, Itoh M
  • Journal Title: Clinical Anatomy 21 Pages: 283-286
  • Peer Reviewed
• [Journal Article] Right gonadal arteries passing dorsally to the inferior vena cava: embryological hypotheses (2008)
  • Author(s): Terayama H, Shuang-Qin Yi, Naito M, Ning Qu, Hirai S, Kitaoka M, Iimura A, Moriyama H, Steinke H, Itoh M
  • Journal Title: Surgical and Radiologic Anatomy 30 Pages: 657-661
  • Peer Reviewed
• [Journal Article] Splenic cytokines in mice immunized with testicular germ cells. (2008)
  • Author(s): Yo Tokunaga, Terayama H, Naito M, Ning Qu, Hirai S, Ogawa Y, Shuang-Qin Yi, Itoh M
  • Journal Title: International Journal of Andrology 31 Pages: 471-476
  • Peer Reviewed
• [Journal Article] A study of the accessory hepatic vein to segments VI and VII with a morphological reconsideration of the human liver (2008)
  • Author(s): Buhe S, Miyaki T, Saito T, Sawuti A, Terayama H, Naito M, Yi SQ, Itoh M
  • Journal Title: Surgical and Radiologic Anatomy 30 Pages: 201-207
  • Peer Reviewed
• [Journal Article] Structure of thymic cysts in congenital lymph nodes lacked mice (2008)
  • Author(s): Nakamura Y, Ning Qu, Terayama H, Naito M, Shuang-Qin Yi, Moriyama H, Itoh M
  • Journal Title: Anatomia Histologia Embryologia 37 Pages: 126-130
  • Peer Reviewed
• [Journal Article] A rare case of inferior mesenteric artery arising from the superior mesenteric artery, with a review of the literature (2008)
  • Author(s): Shuang-Qin Yi, Li J, Terayama H, Naito M, Iimura A, Itoh M.
  • Journal Title: Surgical and Radiologic Anatomy 30 Pages: 159-165
  • Peer Reviewed
• [Journal Article] A rare case of inferior mesenteric artery arising from the superior mesenteric artery, with a review of the literature (2008)
  • Author(s): Shuang-Qin Yi
  • Journal Title: Surgical and Radiologic Anatomy 30 Pages: 159-165
  • Peer Reviewed
• [Journal Article] Structure of thymic cysts in congenital lymph nodes lacked mice (2008)
  • Author(s): Nakamura Y
  • Journal Title: Anatomia Histologia Embryologia 37 Pages: 126-130
  • Peer Reviewed
• [Journal Article] A study of the accessory hepatic vein to segments VI and VII with a morphological reconsideration of the human live (2008)
  • Author(s): Buhe S
  • Journal Title: Surgical and Radiologic Anatatomy 30 Pages: 201-207
  • Peer Reviewed
• [Journal Article] Caput epididymitis but not orchitis was induced by vasectomy in a murine model of experimental autoimmune orchitis (2008)
  • Author(s): Ning Qu
  • Journal Title: Reproduction 135 Pages: 859-866
  • Peer Reviewed
• [Journal Article] Absence of the celiac trunk : Case report and review of the literature (2008)
  • Author(s): Shuang-Qin Yi
  • Journal Title: Clinical Anatomy 21 Pages: 283-286
  • Peer Reviewed
• [Journal Article] Vitamin K2 induces autophagy and apoptosis simultaneously in leukemia cells (2008)
  • Author(s): Yokoyama T
  • Journal Title: Autophagy 4 Pages: 629-640
  • Peer Reviewed
• [Journal Article] Splenic cytokines in mice immunized with testicular germ cells (2008)
  • Author(s): Tokunaga Y
  • Journal Title: International Journal of Andrology 31 Pages: 471-476
  • Peer Reviewed
• [Journal Article] Right gonadal arteries passing dorsally to the inferior vena cava : embryological hypotheses (2008)
  • Author(s): Terayama H
  • Journal Title: Surgical and Radiologic Anatomy 30 Pages: 557-561
  • Peer Reviewed
• [Journal Article] The presence of intra-tubular lymphocytes in normal testis of the mouse (2008)
  • Author(s): Naito M
  • Journal Title: Okajimas Folia Anatomica Japonica 85 Pages: 91-96
  • NAID: 130004495055
  • Peer Reviewed
• [Presentation] 自己免疫性精子形成障害における微小環境の解析 (2010)
  • Author(s): 内藤宗和、平井宗一、寺山隼人、曲寧、北岡三幸、小川夕輝
  • Organizer: 第115回日本解剖学会総会・国学術集会(シンポジウム)
  • Place of Presentation: 岩手医科大学
  • Year and Date: 2010-03-28
• [Presentation] 精巣自己免疫における抗原の解析 (2009)
  • Author(s): 寺山隼人、内藤宗和、曲寧、平井宗一、伊藤正裕
  • Organizer: 日本解剖学会関東支部第19回懇話会(シンポジウム)
  • Place of Presentation: 千葉大学
  • Year and Date: 2009-06-27
• [Presentation] 精巣自己免疫における抗原の解析 (2009)
  • Author(s): 寺山隼人
  • Organizer: 日本解剖学会関東支部第19回懇話会(シンポジウム)
  • Place of Presentation: 葉大学
  • Year and Date: 2009-06-27
• [Presentation] Terminal stage of murine autoimmune orchitis (2009)
  • Author(s): Hirai S, Naito M, Terayama H, Qu N, Itoh M
  • Organizer: 29th Annual Meeting of American Reproductive Immunology
  • Place of Presentation: McCormick Place, Chicago, USA
  • Year and Date: 2009-06-05
• [Presentation] Preliminary characterization of relevant autoantigens in murine testicular autoimmunity (2009)
  • Author(s): Qu N, Naito M, Terayama H, Hirai S, Qu N, Itoh M
  • Organizer: 29th Annual Meeting of American Reproductive Immunology
  • Place of Presentation: McCormick Place, Chicago, USA
  • Year and Date: 2009-06-05
• [Presentation] Proteome analysis of target antigens for testicular autoimmunity in mice (2009)
  • Author(s): Terayama H, Naito M, Hirai S, Qu N, Itoh M
  • Organizer: 29th Annual Meeting of American Reproductive Immunology
  • Place of Presentation: McCormick Place, Chicago, USA
  • Year and Date: 2009-06-05
• [Presentation] Morphological responses in testis, epidydimis and vas deferens following neonatal estrogen treatment (2008)
  • Author(s): 内藤宗和、平井宗一、寺山隼人、曲寧、伊藤正裕
  • Organizer: 日本生殖免疫学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-10-23
• [Presentation] Morphological responses in testis, epidydimis and was deferens following neonatal estrogen treatment (2008)
  • Author(s): 内藤宗和
  • Organizer: 日本生殖免疫学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-10-23
• [Presentation] Morphological responses in testis, epidydimis and vas deferens following neonatal estrogen treatment (2008)
  • Author(s): 内藤宗和、平井宗一、寺山隼人、曲寧、北岡三幸、小川夕輝、川村秀登、伊藤正裕
  • Organizer: 日本生殖免疫学会
  • Place of Presentation: 先斗町歌舞伎練場
  • Year and Date: 2008-07-04
• [Presentation] Morphological responses in testis, epidydimis and was deferens following neonatal estrogen treatment (2008)
  • Author(s): 内藤宗和
  • Organizer: 日本生殖免疫学会
  • Place of Presentation: 先斗町歌舞伎練場
  • Year and Date: 2008-07-04
• [Presentation] マウス新生児期エストロゲン投与による精路系の炎症惹起 (2008)
  • Author(s): 内藤宗和、平井宗一、寺山隼人、曲寧、川村秀登、中野隆、伊藤正裕
  • Organizer: 日本解剖学会
  • Place of Presentation: 岡山理科大学
  • Year and Date: 2008-03-28
• [Remarks]
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• [Remarks]
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• [Remarks]
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