| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2008: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Research Abstract |
Isolated cells from testicular tissues were maintained in serum-free medium, selected growth factors, and STO feeder layer for 1 week. We found that magnetic-activated cell sorting (MACS) was an efficient method to obtain Thy-1+ cells from testis cell suspensions. Enriched Thy-1+ cells were present among unretained cells. Thy-1+ cells were expressed human embryonic stem cell genes. Sixteen weeks after transplanting the Thy-1+ cells into nude mice, there was no evidence of teratoma.
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