The copy number variation analysis of androgenetic hydatidiform mole with high density SNPs array

• Project/Area Number: 20791133
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Chiba University
• Principal Investigator: KAKU Hiroshi Chiba University, 大学院・医学研究部, 協力研究員 (40456059)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 胞状奇胎 / 侵入奇胎 / 雄核発生奇胎 / SNPsアレイ / 遺伝子増幅 / LOH / コピー数変化 / コピーナンバー多型 / 続発症 / マイクロアレイ / 雄核発生 / 雄核

Research Abstract

Hydatidiform mole (HM) occurs in about 1 in 400 pregnancies in Japan. There are two different HM entities: androgenetic diploid 'complete' hydatidiform mole (CHM) and triploid 'partial' hydatidiform mole (PHM). CHM has a higher risk of GTN than PHM. It has been estimated that 10-30% of CHM develops GTN. However, we cannot predict which cases develop to gestational trophoblastic neoplasia. We applied 8 cases complete moles (CHM) to high density SNPs array of Affymetrix, and analyzed the array data with Affymetrix Genotyping Console and Microsoft Excel. 1) Homozygous CHM assessed with STR polymorphism analysis were confirmed the homozygosity in whole genome level. 2) We found specific regions in CHM. 3) We found two GTN specific region in chromosome 2 and 22.

Research Products

• [Journal Article] The risk of post-molar gestational trophoblastic neoplasia is higher in heterozygous than in homozygous complete hydatidiform moles (2010)
  • Author(s): Baasanjav B, Usui H, Kihara M, Kaku H, Nakada E, Tate S, Mitsuhashi A, Matsui H, Shozu M.
  • Journal Title: Hum Reprod 25 Pages: 1182-1191
  • Peer Reviewed
• [Journal Article] The risk of post-molar gestational trophoblastic neoplasia is higher in heterozygous than in homozygous complete hydatidiform moles (2010)
  • Author(s): Baasanjav B, Usui H, Kihara M, Kaku H, Nakada E, Tate S, Mitsuhashi A, Matsui H, Shozu M
  • Journal Title: Hum Reprod (In press)
  • Peer Reviewed
• [Journal Article] 臨床的癒着胎盤の待機療法産婦人科の実際 (2009)
  • Author(s): 加来博志, 尾本暁子, 生水真紀夫
  • Journal Title: 58 Pages: 2035-2041
• [Journal Article] 臨床的癒着胎盤の待機療法 (2009)
  • Author(s): 加来博志, 尾本暁子, 生水真紀夫
  • Journal Title: 産婦人科の実際 58 Pages: 2035-2041
• [Presentation] 出生前羊水染色体検査にてcomplete trisomy 9と診断されたtrisomy 9 mosaicismの1例 (2009)
  • Author(s): 田中宏一, 尾本暁子, 鶴岡信栄, 尾崎江都子, 加来博志, 長田久夫, 生水真紀夫
  • Organizer: 第45回日本周産期・新生児医学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-07-14
• [Presentation] 22q11. 2 deletion症候群合併妊娠の1例 (2009)
  • Author(s): 長田久夫, 尾本暁子, 尾崎江都子, 加来博志, 鶴岡信栄, 田中宏一, 生水真紀夫
  • Organizer: 第45回日本周産期・新生児医学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-07-14
• [Presentation] 出生前羊水染色体検査にて complete trisomy 9と診断された trisomy 9 mosaicism の1例 (2009)
  • Author(s): 田中宏一, 尾本暁子, 鶴岡信栄, 尾崎江都子, 加来博志, 長田久夫, 生水真紀夫
  • Organizer: 第45回日本周産期・新生児医学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-07-14
• [Presentation] 22q11.2 deletion 症候群合併妊娠の1例 (2009)
  • Author(s): 長田久夫, 尾本暁子, 尾崎江都子, 加来博志, 鶴岡信栄, 田中宏一, 生水真紀夫
  • Organizer: 第45回日本周産期・新生児医学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-07-14
• [Presentation] 雄核発生胞状奇胎および絨毛癌培養細胞におけるKvDMRのメチル化状態の解析 (2009)
  • Author(s): 碓井宏和, 木原真紀, 中田恵美里, 鶴岡信栄, 加来博志, 平敷好一郎, 楯真一, 加藤一喜, 田中宏一, 三橋暁, 松井英雄, 生水真紀夫
  • Organizer: 第61回日本産科婦人科学会学術講演会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-04-04
• [Presentation] 癒着胎盤症例に対する子宮動脈塞栓術を用いた待機的治療の有用性 (2008)
  • Author(s): 加来博志, ら
  • Organizer: 第60回日本産科婦人科学会学術講演会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-04-12
• [Remarks]
  • URL: http://www.m.chiba-u.ac.jp/class/gyne/index.html