Research Project
Grant-in-Aid for Young Scientists (B)
We identified novel SF-1/LRH-1 binding sites in human StAR gene about 3,000 bp upstream from the transcriptional start site. We demonstrated by a luciferase reporter system that the upstream region was important for the activation of gene expression. Interestingly, nucleosome eviction has been found at those SF-1/LRH-1 binding sites (both promoter and enhancer region) by ChIP assay. 3C analysis revealed that DNA loop was formed between the promoter and the enhancer regions. These results indicate that the novel enhancer region participates in the human StAR gene activation by orchestrating chromatin loop architecture. Furthermore, we characterized transcription factor C/EBPβ which is identified as one of the components of SF-1 complex by biochemical approach. We elucidated C/EBPβ is also important for SF-1/LRH-1-induced ovarian differentiation as a these partner.
All 2010 2009 2008 Other
All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (24 results) Remarks (1 results)
Mol. Endocrinol. 24(3)
Pages: 485-496
Mol.Endocrinol. 24(3)
Endocrinology 150(8)
Pages: 3885-3893
Biol. Endocrinol. 16:6(1)
Pages: 62-62
Reprod. Biol. Endocrinol 16 : 6(1)
http://www.med.u-fukui.ac.jp/SEIKA2/
