The basic study of the "reprogramming therapy of cancer using the cytosol of oocytes and ES cells.

• Project/Area Number: 20791140
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: University of Yamanashi
• Principal Investigator: FUKASAWA Hiroko University of Yamanashi, 大学院・医学工学総合研究部, 助教 (60362068)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 癌 / 体細胞核移植 / クローン胚 / ES細胞 / ntES細胞

Research Abstract

Although the rate of development of cloned embryos to the blastocyst stage using the treated oocytes was lower than that obtained using fresh oocytes, three live pups were delivered after embryo transfer into pseudopregnant females (0.4% of the oocytes used). Thus, although cryopreservation reduces the potential of oocytes, these cells retain the ability to support the full-term development of cloned embryos.

Research Products

• [Journal Article] Generation of healthy cloned mice using enucleated eggs preserved oocytes. (2011)
  • Author(s): Hirata S, Fukasawa H, Wakayama S, Wakayama T, Hoshi K
  • Journal Title: Cell Reprogram 13(1) Pages: 7-11
  • Peer Reviewed
• [Journal Article] Generation of healthy cloned mice using enucleated cryopresereved oocytes. (2011)
  • Author(s): Hirata S.
  • Journal Title: Cellular Reprogramming Volume: 13 Pages: 7-11
  • Peer Reviewed
• [Journal Article] 未受精卵の細胞室の初期化能に凍結-融解が与える影響 (2011)
  • Author(s): 深澤宏子
  • Journal Title: 日本産科婦人科学会雑誌 Volume: 63 Pages: 671-671
• [Journal Article] 未受精卵の核および細胞質に凍結-融解が与える影響 (2010)
  • Author(s): 深澤宏子
  • Journal Title: 日本産科婦人科学会雑誌 62巻 Pages: 733-733
• [Journal Article] Novel alpha fetoprotein-V mRNA isoform in human. (2009)
  • Author(s): Tagaya H, Fukasawa H, Shoda T, Hirata S, Hoshi K
  • Journal Title: Reprod Sci 16(8) Pages: 794-801
  • Peer Reviewed
• [Journal Article] 未受精卵の細胞質に凍結が及ぼす影響-凍結除核未受精卵を用いた体細胞クローン胚による検討. (2008)
  • Author(s): 深澤宏子, 多賀谷光, 正田朋子, 笠井剛, 平田修司, 星和彦
  • Journal Title: 日本生殖医学会雑誌(シンポジウム) 53(4),99(223)
• [Journal Article] Effects of sirtinol on early development of the cloned murine embryo. (2008)
  • Author(s): Hirata S, Fukasawa H, Tagaya H, Shoda T, Wakayama T, Hoshi K
  • Journal Title: Yamanashi Med J 23(4) Pages: 97-107
  • NAID: 110007018284
  • Peer Reviewed
• [Journal Article] Effects of sirtinol on early development of the cloned murine embryo (2008)
  • Author(s): Hirata S, Fukasawa H, Tagaya H, Shoda T, Wakayama T, Hoshi K
  • Journal Title: Yamanashi Med J 23 Pages: 97-107
  • NAID: 110007018284
  • Peer Reviewed
• [Presentation] MESI法を用いた1-day-old卵の紡錘体ならびに細胞質の機能の検討 (2010)
  • Author(s): 深澤宏子
  • Organizer: 第55回日本生殖医学会学術講演会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-11
• [Presentation] Spindle replacement of oocyte by metaphase II spindle injection (MESI). (2010)
  • Author(s): Fukasawa H, Fujie M, Mabuchi T, Hirata S
  • Organizer: The 7th Conference of Asian Society for Mitochondrial Rearch and Medicin and The 10th Conference of Japanese Society of Mitochondrial Rearch and Medicin.
  • Place of Presentation: Fukuoka
• [Presentation] MESI法を用いた1-day-old 卵の紡錘体ならびに細胞質の機能の検討 (2010)
  • Author(s): 深澤宏子, 藤江道子, 平田修司
  • Organizer: 第55 回日本生殖医学会学術講演会
  • Place of Presentation: 徳島
• [Presentation] 未受精卵の核および細胞質に凍結-融解が与える影響 (2010)
  • Author(s): 深澤宏子, 藤江道子, 平田修司, 星和彦
  • Organizer: 第62回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京
• [Presentation] 未受精卵の細胞質に凍結が及ぼす影響-凍結除核未受精卵を用いた体細胞クローン胚による検討 (2008)
  • Author(s): 深澤宏子, 平田修司, 多賀谷光, 正田朋子, 笠井剛, 星和彦
  • Organizer: 第53回日本生殖医学会学術講演会(シンポジウム)
  • Place of Presentation: 神戸
  • Year and Date: 2008-10-23
• [Presentation] 未受精卵の細胞質に凍結が及ぼす影響-凍結除核未受精卵を用いた体細胞クローン胚による検討 (2008)
  • Author(s): 深澤宏子, 多賀谷光, 正田朋子, 笠井剛,平田修司, 星和彦
  • Organizer: 第53 回日本生殖医学会学術講演会・総会
  • Place of Presentation: 神戸(シンポジウム)