| Project/Area Number |
20791142
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University |
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Principal Investigator |
MIYATAKE Takashi Osaka University, 医学部附属病院, 助教 (70448067)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 婦人科腫瘍学 / p72 / p68 / β-カテニン / 子宮頸癌 |
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| Research Abstract |
The p72 expression on cervical cancer specimens are evaluated by real-time PCR and compared with p72 on normal uterine cervix. The results show no difference. The p72 protein expression are also evaluated with immunostaining and p72 related genes, p68 and β-catenin are compared with p72 expression level. The protein expression of p72 is significantly low in cervical cancer, however, expressions of p68 andβ-catenin are not tightly related with p72 expression. The results so far cannot conclude the function of p72 on cervical carcinogenesis.
A cervical cancer cell line, which is introduced p72 gene, are made and the protein expression was compared with the original cell line with 2-dimensional electrophoresis. We detected 20 proteins, whose expression level has changed with the introduction of p72. Now we are further targeting a protein which is directly related with carcinogenesis.
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