| Project/Area Number |
20791158
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Iwate Medical University |
|---|
Principal Investigator |
NAGASAWA Takayuki Iwate Medical University, 医学部, 助教 (10453309)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
|---|
| Keywords | 子宮体癌 / 腺管分離 / 遺伝子解析 |
|---|
| Research Abstract |
We analyzed molecular abnormarityies of single tumor glands using crypt isolation method in 25 endometrial endometrioid adenocarcinoma patients. In addition, we investigated the relation between the form of molecular abnormalities among single tumor gland and the disease prognosis. The frequency of LOH in the pooled gland sample obtained using crypt isolation was higher in many loci than that in the sample obtained using the conventional method, in which crypt isolation was not used. This suggests that by analyzing a pure sample of only tumor glands obtained using crypt isolation, gene abnormalities that cannot be detected in samples obtained by the conventional method can be detected and that the analysis sensitivity is improved. With the exception of local recurrence, progression of cancer during therapy or recurrence after therapy was observed in 4 cases. From the view point of genetic abnormality, the 3 cases had LOH in a plurality of regions and heterogeneity among single tumor glands. Among the other case, although LOH was not found in the pooled gland sample, it was only found in some single tumor gland samples. All 4 cases had LOH in the 5 q loci at least in a single tumor gland. This suggests the possibility that although LOH in the 5 q has a minor role in endometrial carcinogenesis, it is a poor prognostic factor. Heterogeneity of gene abnormality between single tumor glands was confirmed in 16 (64%) of the 25 cases, indicating that endometrial carcinoma consists of various single tumor glands with different molecular abnormalities. In addition, it was suggested that the form of gene abnormalities in individual tumor glands is related to sensitivity and resistance to chemotherapy.
|
