Analysis of immune escape mechanism in cervical cancer that aims at immunotherapeutic application

Research Project

Project/Area Number	20791163
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Obstetrics and gynecology
Research Institution	Keio University
Principal Investigator	NISHIO Hiroshi Keio University, 医学部, 助教 (90445239)
Project Period (FY)	2008 – 2009
Project Status	Completed (Fiscal Year 2009)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	子宮頸癌 / 腫瘍免疫学 / 免疫逃避 / B7ファミリー / 免疫抑制 / シグナル伝達系 / MAPK / HPV
Research Abstract	Immune suppressive molecules including PD-L1 and B7-DC was evaluated in cervical cancer cell lines by ELISA or FACS method. Activation of intracellular signaling pathways was detected by western blotting method. As a result, PD-L1 and B7-DC protein in SKGII cells was depend on Erk1/2 cascade specifically and not associated with HPV E6/E7 protein.