Generating Synthetic Anti-HPV18 E7 Peptide
| Project/Area Number |
20791167
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Keio University |
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Principal Investigator |
ONO Akiko Keio University, 医学部, 研究員(非常勤) (70383883)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 癌 / ウイルス / タンパク質 / 蛋白質 |
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| Research Abstract |
Quantitative binding assay system to investigate the interaction between HPV18 E7 and RB was constructed as expression plasmids. Peptide construct including LxCxE motif showed a certain but labile amount of inhibitory effect .Adjustment of the peptide by adding a pentamerization domain showed stronger inhibitory effect. Cell viability assay did not show significant difference due to poor plasmid transfection efficiency. Adenovirus vectors expressing the pentamerized peptides were constructed for improvement.
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Report
(3 results)
Research Products
(3 results)
