The functional analysis of the mensetruation-related genes and its role in the onset and the progression of the endometriosis.

• Project/Area Number: 20791168
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: ODA Hideyuki Keio University, 医学部, 研究員 (50445235)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 月経関連遺伝子 / 子宮内膜症 / sarcoglycan-delta / 磁力 / 子宮内膜症モデルマウス / microarray / 応用動物 / 幹細胞 / in vitro月経モデル / delta sarcoglycan

Research Abstract

We have already had the candidate genes of the mensetruation-related genes which expression alters accoding to the menstrual period. And we found that the expression of the sarcoglycan-delta increased not only by the microarray but also by RT-PCR and immunohistochemistry. And to analyze its effect in the endometriosis we try to develop the novel endeomtriosis model mouse using the magnetic power and improve the magnet and magnetic materials.

Research Products

• [Journal Article] Stem Cell-Like Properties of the Endometrial Side Population : Implication in Endometrial Regeneration. (2010)
  • Author(s): Masuda H, Oda H, Yoshimura Y, Maruyama T
  • Journal Title: PLoS ONE volume 5(4)
  • Peer Reviewed
• [Journal Article] OCT4 expression in human uterine myometrial stem/progenitor cells. (2010)
  • Author(s): Ono M, Oda H, Yoshimura Y, Maruyama T
  • Journal Title: Hum Reprod volume25(8) Pages: 2059-2067
  • Peer Reviewed
• [Journal Article] OCT4 expression in human uterine myometrial stem/progenitor cells. (2010)
  • Author(s): Ono M, Oda H, et al.
  • Journal Title: Hum Reprod. Volume: 25 Pages: 2059-2067
  • Peer Reviewed
• [Journal Article] Stem Cell-Like Properties of the Endometrial Side Population : Implication in Endometrial Regeneration. (2010)
  • Author(s): Masuda H, Oda H, et al.
  • Journal Title: PLoS ONE. Volume: 5
  • Peer Reviewed
• [Journal Article] Stem cell-like properties of the endometrial Side population : implication in endometrial regeneration. (2010)
  • Author(s): Masuda H, Matsuzaki Y, Hiratsu E, Ono M, Nagashima T, Kajitani T, Arase T, Oda H, Uchida H, Asada H, lto M, Yoshimura Y, Maruyama T, Okano H
  • Journal Title: PLoS ONE. 5
  • Peer Reviewed
• [Journal Article] Epigenetic Treatment in Assisted Human Embryo Implantation. (2009)
  • Author(s): Uchida H, Maruyama T, Oda H
  • Journal Title: J.Mamm.Ova Res. volume26 Pages: 116-121
  • NAID: 130004436751
  • Peer Reviewed
• [Journal Article] The UDP-glucose recepter P2RY14 triggers innate mucosal immunity in the female reproductive tract by inducing IL-8. (2009)
  • Author(s): Arase T, Oda H, Maruyama T
  • Journal Title: J Immunol. volume182 Pages: 7074-7084
  • Peer Reviewed
• [Journal Article] Epigenetic Treatment in Assisted Human Embryo Implantation. (2009)
  • Author(s): Uchida H, Maruyama T, Kagami M, Oda H, Nishikawa S, Kajitani T, Asada H, Yoshimura Y
  • Journal Title: J.Mamm.Ova Res. 26 Pages: 116-121
  • NAID: 130004436751
  • Peer Reviewed
• [Journal Article] The UDP-glucose recepter P2RY14 triggers innate mucosal immunity in the female reproductive tract by inducing IL-8. (2009)
  • Author(s): Arase T, Uchida H, Kajitani T, Ono M, TamakiK, Oda H, Nishikawa S, Kagami M, Nagashima T, Masuda H, Asada H, Yoshimura Y, Maruyama T
  • Journal Title: J Immunol. 182 Pages: 7074-7084
  • Peer Reviewed
• [Journal Article] Activation of SRC kinase and phosphorylation of signal transducer and activator of transcription-5 are required for decidual transformation of human endometrial stromal cells. (2008)
  • Author(s): Nagashima T, Maruyama T, Oda H
  • Journal Title: Endocrinology volume149 Pages: 1227-1234
  • Peer Reviewed
• [Journal Article] Glycodelin blocks progression to S phase and inhibits cell growth : a possible progesterone-induced regulator for endometrial epithelial cell growth. (2008)
  • Author(s): Ohta K, Maruyama T, Oda H
  • Journal Title: Molecular human reproduction volume14 Pages: 17-22
  • Peer Reviewed
• [Journal Article] 【思春期の諸問題】排卵障害 (2008)
  • Author(s): 丸山哲夫, 小田英之, 西川明花, 各務真紀, 内田浩, 吉村泰典
  • Journal Title: 産科と婦人科 75巻 Pages: 529-536
  • Peer Reviewed
• [Journal Article] 【産婦人科ホルモン療法マニュアル】生殖内分泌・不妊 無月経 (2008)
  • Author(s): 丸山哲夫, 小田英之, 吉村泰典
  • Journal Title: 産科と婦人科 75巻 Pages: 8-14
  • Peer Reviewed
• [Journal Article] 【ホルモン療法 最近の話題】月経異常を伴う内分泌疾患 (2008)
  • Author(s): 内田浩, 小田英之, 吉村泰典
  • Journal Title: 産婦人科治療 96巻 Pages: 163-168
  • Peer Reviewed
• [Journal Article] 妊娠子宮における子宮筋Side Population細胞の役割 (2008)
  • Author(s): 小野政徳, 丸山哲夫, 小田英之, 吉村泰典
  • Journal Title: 日本生殖内分泌学会雑誌 13巻 Pages: 25-19
  • NAID: 10021262312
  • Peer Reviewed
• [Journal Article] 子宮内膜症・腺筋症由来疼痛に関連する遺伝子の発現解析 (2008)
  • Author(s): 梶谷宇, 丸山哲夫, 小田英之, 吉村泰典
  • Journal Title: エンドメトリオージス研究会会誌 29巻 Pages: 91-93
  • Peer Reviewed
• [Journal Article] Activation of SRC kinase and phosphorylation of signal transducer and activator of transcription-5 are required for decidual transformation of human endometrial stromal cells (2008)
  • Author(s): Nagashima T, Maruyama T, Uchida H, Kajitani T, Arase T, Ono M, Oda H, Kagami M, Masuda H, Nishikawa S, Asada H, Yoshimura Y
  • Journal Title: Endocrinology 149 Pages: 1227-1234
  • Peer Reviewed
• [Journal Article] Glycodelin blocks progression to S phase and inhibits cell growth : a possible progesterone-induced regulator for endometrial epithelial cell growth (2008)
  • Author(s): Ohta K, Maruyama T, Uchida H, Ono M, Nagashima T, Arase T, Kajitani T, Oda H, Morita M, Yoshimura Y
  • Journal Title: Molecular human reproduction 14 Pages: 17-22
  • Peer Reviewed
• [Journal Article] 【思春期の諸問題】排卵障害 (2008)
  • Author(s): 丸山哲夫, 小田英之, 西川明花, 各務真紀, 内田浩, 吉村泰典
  • Journal Title: 産科と婦人科 75 Pages: 529-536
  • Peer Reviewed
• [Journal Article] 【産婦人科ホルモン療法マニュアル】生殖内分泌・不妊無月経 (2008)
  • Author(s): 丸山哲夫, 西川明花, 小田英之, 荒瀬透, 小野政徳, 各務真紀, 内田浩, 吉村泰典
  • Journal Title: 産科と婦人科 75 Pages: 8-14
  • Peer Reviewed
• [Journal Article] 【ホルモン療法最近の話題】月経異常を伴う内分泌疾患 (2008)
  • Author(s): 内田浩, 荒瀬透, 小野政徳, 各務真紀, 小田英之, 西川明花, 丸山哲夫, 吉村泰典
  • Journal Title: 産婦人科治療 96 Pages: 163-168
  • Peer Reviewed
• [Journal Article] 妊娠子宮における子宮筋Side Population細胞の役割 (2008)
  • Author(s): 小野政徳, 丸山哲夫, 梶谷宇, 内田浩, 荒瀬透, 各務真紀, 小田英之, 西川明花, 升田博隆, 長島隆, 太田邦明, 伊藤守, 浅田弘法, 岡野栄之, 松崎有未, 吉村泰典
  • Journal Title: 日本生殖内分泌学会雑誌 13 Pages: 25-29
  • NAID: 10021262312
  • Peer Reviewed
• [Journal Article] 子宮内膜症・腺筋症由来疼痛に関連する遺伝子の発現解析 (2008)
  • Author(s): 梶谷宇, 丸山哲夫, 浅田弘法, 小野政徳, 荒瀬透, 小田英之, 西川明花, 内田浩, 吉村泰典
  • Journal Title: エンドメトリオージス研究会会誌 29 Pages: 91-93
  • Peer Reviewed
• [Presentation] Possible involvemen t of endometrial stem/progenitor cells in the pathogenesis of endometriosis. (2010)
  • Author(s): Tetsuo Maruyama
  • Organizer: The First Asian Conference on Endometriosis (ACE)
  • Place of Presentation: Denver, USA
• [Presentation] Prospective isolation and functional analysis of stem/progenitor cells from the human uterine myometrium. (2010)
  • Author(s): Masanori Ono
  • Organizer: 8th International Society for Stem Cell Reserch (ISSCR)
  • Place of Presentation: San Francisco, CA USA
• [Presentation] 〔招請講演〕Possible involvement of endometrial stem/progenitor cells in the pathogenesis of endocetriosis. (2010)
  • Author(s): Tetsuo Maruyama, Hideyuki Oda, et al.
  • Organizer: The First Asian Conference on Endometriosis (ACE).
  • Place of Presentation: Denver, USA
• [Presentation] Prospective isolation and functional analysis of stem/progenitor cells from the human uterine myometrium. (2010)
  • Author(s): Masanori Ono, Hideyuki Oda, et al.
  • Organizer: 8th International Society for Stem Cell Research (ISSCR).
  • Place of Presentation: San Francisco, CA USA
• [Presentation] Up-regulation of 5-and 15-lipoxygenases in endometriosis. (2009)
  • Author(s): Takashi Kajitani
  • Organizer: 25th European Society of Human Reproduction and Embryology (ESHRE)
  • Place of Presentation: Amsterdam, Netherlands
• [Presentation] UDP-glucose and its receptor P2RY14 as a novel innate immunity in the female reproductive tract. (2009)
  • Author(s): Toru Arase
  • Organizer: 29th Ameican Society for Reproductive Immunology (ASRI)
  • Place of Presentation: Orlando, Florida
• [Presentation] The role of uterine smooth muscle stem cells, isolated based on cell surface markers, in myometrial remodeling in the pregnant uterus. (2009)
  • Author(s): Ono M
  • Organizer: 56th Society for Gynecologic Investigation
  • Place of Presentation: Glasgow, Scotland
• [Presentation] 表面抗原を用いたヒト子宮平滑筋幹細胞の新しい分離法の開発とその妊娠至急リモデリングにおける役割 (2009)
  • Author(s): 小野政徳
  • Organizer: 第8回 日本再生医療学会
  • Place of Presentation: 東京都千代田区
• [Presentation] 子宮内膜症組織におけるリポキシゲナーゼ経路関連遺伝子の発現解析 (2009)
  • Author(s): 梶谷宇
  • Organizer: 第30回 日本エンドメトリオーシス学会
  • Place of Presentation: 宮城県仙台市
• [Presentation] Up-regulation of 5- and 15-lipoxygenases in endometriosis. (2009)
  • Author(s): Takashi Kajitani, Tetsuo Maruyama, Hironori Asada, Akiko Yamasaki, Hideyuki Oda, Sayaka Nishikawa, Hiroshi Uchida, Yasunori Yoshimura
  • Organizer: 25th European Society of Human Reproduction and Embryology(ESHRE).
  • Place of Presentation: Amsterdam, Netherlands
• [Presentation] UDP-glucose and its receptor P2RY14 as a novel innate immunity in the female reproductive tract. (2009)
  • Author(s): Toru Arase, Tetsuo Maruyama, Hiroshi Uchida, Takashi Kajitani, Sayaka Nishikawa, Hideyuki Oda, Maki Kagami, Hironori Asada, Yasunori Yoshimura
  • Organizer: 29th Ameican Society for Reproductive Immunology(ASRI)
  • Place of Presentation: Orlando, Florida
• [Presentation] The role of uterine smooth muscle stem cells, isolated based on cell surface markers, in myometrial remodeling in the pregnant uterus (2009)
  • Author(s): Ono M, Maruyama T, Kajitani T, Uchida H, Oda H, Nishikawa S, Kagami M, Arase T, Nagashima T, Masuda H, Morikawa S, Mabuchi Y, Okano Y, Matsuzaki Y, Yoshimura Y
  • Organizer: 56th Society for Gynecologic Investigation
  • Place of Presentation: Glasgow, Scotland
• [Presentation] 表面抗原を用いたヒト子宮平滑筋幹細胞の新しい分離法の開発とその妊娠至急リモデリングにおける役割 (2009)
  • Author(s): 小野政徳, 丸山哲夫, 梶谷宇, 内田浩, 荒瀬透, 小田英之, 西川明花, 各務真紀, 浅田弘法, 升田博隆, 岡野栄之, 松崎有未, 吉村泰典
  • Organizer: 第8回日本再生医療学会
  • Place of Presentation: 東京都千代田区
• [Presentation] 子宮内膜症組織におけるリポキシゲナーゼ経路関連遺伝子の発現解析 (2009)
  • Author(s): 梶谷宇, 丸山哲夫, 浅田弘法, 内田浩, 各務真紀, 小田英之, 西川明花, 山崎彰子, 吉村泰典
  • Organizer: 第30回日本エンドメトリオーシス学会
  • Place of Presentation: 宮城県仙台市
• [Presentation] 子宮内膜におけるP2RY14を介した新たな粘膜防御機構 (2008)
  • Author(s): 荒瀬透
  • Organizer: 第23回 日本生殖免疫学会
  • Place of Presentation: 富山県富山市
• [Presentation] Chemial abortionの既往を有する反復流産患者の病院および妊娠転帰に関する検討 (2008)
  • Author(s): 西川明花
  • Organizer: 第53回 日本生殖医学会
  • Place of Presentation: 神戸市中央区
• [Presentation] 子宮平滑筋幹細胞の新しい分離法とその機能解析 (2008)
  • Author(s): 小野政徳
  • Organizer: 第81回 日本内分泌学会学術総会
  • Place of Presentation: 青森県弘前市
• [Presentation] 子宮平滑筋幹細胞の新しい分離法とその機能解析 (2008)
  • Author(s): 小野政徳, 丸山哲夫, 梶谷宇, 内田浩, 荒瀬透, 小田英之, 西川明花, 長島隆, 升田博隆, 馬渕洋, 森川暁, 岡野栄之, 松崎有未, 吉村泰典
  • Organizer: 第81回日本内分泌学会学術総会
  • Place of Presentation: 青森県弘前市
• [Presentation] 子宮内膜におけるP2RY14を介した新たな粘膜防御機構. (2008)
  • Author(s): 荒瀬透, 丸山哲夫, 内田浩, 梶谷宇, 西川明花, 小田英之, 各務真紀, 浅田弘法, 吉村泰典
  • Organizer: 第23回日本生殖免疫学会
  • Place of Presentation: 富山県富山市
• [Presentation] Chemial abortionの既往を有する反復流産患者の病院および妊娠転帰に関する検討 (2008)
  • Author(s): 西川明花, 丸山哲夫, 小田英之, 各務真紀, 荒瀬透, 小野政徳, 長島隆, 内田浩, 吉村泰典
  • Organizer: 第53回日本生殖医学会
  • Place of Presentation: 神戸市中央区