Proteomics to identify the targets of antiphospholipid antibodies in women with recurrent pregnancy loss

• Project/Area Number: 20791171
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Nippon Medical School
• Principal Investigator: MINE Katsuya Nippon Medical School, 医学部, 助教 (60409216)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: プロテオミクス / 習慣流産 / 抗リン脂質抗体 / 不育症 / 二次元電気泳動

Research Abstract

Blood plasma samples were collected from women with recurrent pregnancy loss. Further, a clinical database of these patients was constructed.
Solid-phase plates comprising phospholipids embedded within cell membranes were constructed to extract phospholipids that bind to plasma proteins. The blood plasma samples were reacted with the plates to isolate the target plasma proteins. These proteins were visualized by 2-dimensional electrophoresis (2-DE). The protein spots on the 2-DE gels were identified by mass spectrometry.

Research Products

• [Journal Article] 常位胎盤早期剥離と抗リン脂質抗体症候群 (2009)
  • Author(s): 峯克也
  • Journal Title: 産婦人科の実際 58 Pages: 2107-2112
• [Journal Article] アスピリン・ヘパリン療法中に絨毛膜下血腫を呈し、アスピリン中止後IUFDに至った胎児腹壁破裂症例 (2009)
  • Author(s): 峯克也
  • Journal Title: 日本受精着床学会雑誌 27 Pages: 252-255
  • Peer Reviewed
• [Journal Article] Oocyte evaluation based on hexanoyl-lysine in follicular fluid in sterile women
  • Author(s): 峯克也、富山僚子、桑原慶充、稲川智子、阿部崇、西弥生、明楽重夫、佐藤嘉兵、竹下俊行
  • Journal Title: 日本受精着床学会雑誌 26巻第一号 Pages: 114-117
  • Peer Reviewed
• [Journal Article] 常位胎盤早期剥離と抗リン脂質抗体症候群
  • Author(s): 峯克也, 市川智子, 竹下俊行
  • Journal Title: 産婦人科の実際 58巻13号 Pages: 2107-2112
• [Journal Article] アスピリン・ヘパリン療法中に絨毛膜下血腫を呈し、アスピリン中止後IUFDに至った胎児腹壁破裂症例
  • Author(s): 峯克也、桑原慶充、稲川智子、阿部崇、富山僚子、西弥生、明楽重夫、竹下俊行
  • Journal Title: 日本受精着床学会雑誌 27巻第一号 Pages: 252-255
  • Peer Reviewed
• [Presentation] アスピリン・ヘパリン療法中に絨毛膜下血腫を呈し、アスピリン中止後IUFDに至った胎児腹壁破裂症例 (2009)
  • Author(s): 峯克也、桑原慶充、稲川智子、阿部崇、富山僚子、西弥生、明楽重夫、竹下俊行
  • Organizer: 第27回受精着床学会
  • Place of Presentation: 京都
  • Year and Date: 2009-08-06
• [Presentation] 卵胞内酸化-抗酸化バランスの臨床的検討 (2009)
  • Author(s): 峯克也、富山僚子、阿部崇、稲川智子、桑原慶充、西弥生、明楽重夫、佐藤嘉兵、竹下俊行
  • Organizer: 第61回日本産婦人科学会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-04-04
• [Presentation] 卵胞内酸化・抗酸化バランスの臨床的検討 (2009)
  • Author(s): 峯克也
  • Organizer: 日本産婦人科学会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-04-04
• [Presentation] GnRHアンタゴニストは卵胞内のhypoxiaを誘導する (2008)
  • Author(s): 峯克也
  • Organizer: 第26回受精着床学会
  • Place of Presentation: 福岡
  • Year and Date: 2008-08-28