Establishment of vascular endothelial cell culture system using human placental cells

• Project/Area Number: 20791174
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: MAKINO Hatsune National Research Institute for Child Health and Development, 生殖・細胞医療研究部共, 同研究員 (90392498)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 生殖医療 / 発生・分化 / 発現制御 / 発現抑制

Research Abstract

This study was performed to isolate novel cells useful for prevention of various cardiovascular diseases and to develop clinical application of these cells. I demonstrated that cells derived from human amniotic mesoderm, chorionic plate, and villous chorion efficiently transdifferentiated into myotubes, and I demonstrated that human amniotic membrane-derived MSC had a high ability to transdifferentiate into cardiomyocytes. Moreover, I revealed that human placental artery-derived endothelial cells efficiently transdifferentiated into myoblasts/myocytes.

Research Products

• [Journal Article] Dystrophin conferral using human endothelium expressing HLA-E in the non-immunosuppressive murine model of Duchenne muscular dystrophy. (2011)
  • Author(s): Cui CH, Miyoshi S, Tsuji H, Makino H, Kanzaki S, Kami D, Terai M, Suzuki H, Umezawa A.
  • Journal Title: Hum Mol Genet. 20(2) Pages: 235-244
• [Journal Article] Defining hypo-methylated regions of stem cell-specific promoters in human iPS cells derived from extra-embryonic amnions and lung fibroblasts. (2010)
  • Author(s): Nishino K, Toyoda M, Yamazaki-Inoue M, Makino H, Fukawatase Y, Chikazawa E, Takahashi Y, Miyagawa Y, Okita H, Kiyokawa N, Akutsu H, Umezawa A.
  • Journal Title: PLoS One. 5(9)
• [Journal Article] Isolation and Culture of Human Mesenchymal Stem Cells. (2010)
  • Author(s): Umezawa A, Makino H.
  • Journal Title: Organ Biology. 17(1) Pages: 67-75
• [Journal Article] Xenografted human amniotic membrane-derived mesenchymal stem cells are immunologically tolerated and transdifferentiated into cardiomyocytes. (2010)
  • Author(s): Tsuji H, Miyoshi S, Ikegami Y, Hida N, Asada H, Togashi I, Suzuki J, Satake M, Nakamizo H, Tanaka M, Mori T, Segawa K, Nishiyama N, Inoue J, Makino H, Miyado K, Ogawa S, Yoshimura Y, Umezawa A.
  • Journal Title: Circ Res. 106(10) Pages: 1613-1623
• [Journal Article] Cells of extraembryonic mesodermal origin confer human dystrophin in the mdx model of Duchenne muscular dystrophy. (2010)
  • Author(s): Kawamichi Y, Cui CH, Toyoda M, Makino H, Horie A, Takahashi Y, Matsumoto K, Saito H, Ohta H, Saito K, Umezawa A.
  • Journal Title: J Cell Physiol. 223(3) Pages: 695-702
• [Journal Article] Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells. (2009)
  • Author(s): Nagata S, Toyoda M, Yamaguchi S, Hirano K, Makino H, Nishino K, Miyagawa Y, Okita H, Kiyokawa N, Nakagawa M, Yamanaka S, Akutsu H, Umezawa A, Tada T.
  • Journal Title: Genes Cells. 14(12) Pages: 1395-1404
• [Journal Article] Human sclera maintains common characteristics with cartilage throughout evolution. (2008)
  • Author(s): Seko Y, Azuma N, Takahashi Y, Makino H, Morito T, Muneta T, Matsumoto K, Saito H, Sekiya I, Umezawa A.
  • Journal Title: PLoS One. 3(11)
• [Journal Article] Gremlin enhances the determined path to cardiomyogenesis. (2008)
  • Author(s): Kami D, Shiojima I, Makino H, Matsumoto K, Takahashi Y, Ishii R, Naito AT, Toyoda M, Saito H, Watanabe M, Komuro I, Umezawa A.
  • Journal Title: PLoS One. 3(6)
• [Journal Article] 細胞ソースの確立 (2008)
  • Author(s): 梅澤明弘、牧野初音
  • Journal Title: 日本臨床(特集:再生医学と医療-幹細胞の基礎研究と臨床の進歩) Vol.66、5巻 Pages: 865-872