Bral2 is essential for the maintenance of the extracellular microenvironment for the synaptic transmission in the auditory system.

• Project/Area Number: 20791195
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: Okayama University
• Principal Investigator: BEKKU Yoko Okayama University, 医学部, 技術専門職員 (60397875)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 神経科学 / 聴覚伝導路 / 細胞外マトリックス / 神経細胞周囲網 / プロテオグリカン / リンクプロテイン

Research Abstract

There are two types of cells in the brain ; the neuronal cell and glial cell. Extracellular matrix (ECM), such as hyaluronan and proteoglycan, exists in extracellular region of these cells. There are two dense organized ECM in the brain, one is in the perineuronal net (PNN) and other is at the node of Ranvier. The PNN is a mesh like structure surrounding the neuronal cell. Bral2 forms ECM complex with hyaluronan and proteoglycan, and is localized in the PNN of the auditory system. In this study, it is strongly suggested that Brals play key roles in the ECM assembly and the complex is essential for the neuronal activity through the analysis of Bral - deficient mice

Research Products

• [Journal Article] its role in diffusion barrier formation and conduction velocity in the CNS (2010)
  • Author(s): Bekku Y, Vargova L, Goto Y, Vorisek I, Dmytrenko L, Narasaki M, Ohtsuka A, Fassler R, Ninomiya Y, Sykova E, Oohashi T, Bral1
  • Journal Title: J Neurosci 30(8) Pages: 3113-3123
  • Peer Reviewed
• [Journal Article] Brall : its role in diffusion barrier formation and conduction velocity in the CNS. (2010)
  • Author(s): Bekku, et al.
  • Journal Title: J Neurosci 30 Pages: 31133123-31133123
  • Peer Reviewed
• [Journal Article] Brevican organizes hyruronan-binding extracellular assemblies distinctively at the large diameter nodes of Ranvier in the CNS (2009)
  • Author(s): Bekku Y, Ninomiya Y, Oohashi T
  • Journal Title: Neurosci Res 65
• [Journal Article] Brevican distinctively assembles extracellular components at the large diameter nodes of Ranvier in the CNS (2009)
  • Author(s): Bekku Y, Rauch U, Ninomiya Y, Oohashi T
  • Journal Title: J Neurochem 108(5) Pages: 1266-76
  • Peer Reviewed
• [Journal Article] Brevican distinctively assembles extracellular components at the large diameter nodes of Ranvier in the CNS. (2009)
  • Author(s): Bekku Y, et al.
  • Journal Title: J Neurochem 108 Pages: 1266-1276
  • Peer Reviewed
• [Journal Article] Molecular cloning and developmental expression of a hyaluronan and proteoglycan link protein gene, crtl1/hapln1, in zebrafish (2008)
  • Author(s): Kang JS, Kawakami Y, Bekku Y, Ninomiya Y, Belmonte JCI, Oohashi T
  • Journal Title: Zoological Science Vol25 Pages: 912-918
  • NAID: 40016386641
  • Peer Reviewed
• [Presentation] Brevican organizes hyaluronan-binding extracellular assemblies distinctively at the large diameter nodes of Ranvier in the CNS (2009)
  • Author(s): Bekku Y, Rauch U, Ninomiya Y, Oohashi T
  • Organizer: Neuroscience 2009第32回日本神経科学会大会
  • Place of Presentation: 名古屋
• [Presentation] The nodal ECM barrier model in the CNS: From an extracellular ion diffusion analysis in Bral1-deficient mice (2009)
  • Author(s): Bekku Y, Vargova L, Goto Y, Vorisek I, Fassler R, Ninomiya Y, Sykova E, Oohashi T
  • Organizer: Ninth Biennial Satellite Meeting of the ISN on Myelin Biology, Myelin Development, Function and Related Diseases Conference
  • Place of Presentation: Gyeongju, S. Korea
• [Presentation] The ion-diffusion barrier model at the node of Ranvier: From an extracellular ion diffusion analysis in Bral1-deficient mice (2009)
  • Author(s): Bekku Y, Vargova L, Goto Y, Ninomiya Y, Sykova E, Oohashi T
  • Organizer: 第36回国際生理学会大会
  • Place of Presentation: 京都
• [Presentation] Brevican determines specialization of the hyaluronan-binding nodal matrix assemblies at the large diameter nodes of Ranvier in the CNS (2009)
  • Author(s): Bekku Y, Rauch U, Ninomiya Y, Oohashi T
  • Organizer: 第56回マトリックス研究会大会、8th Pan-Pacific Connective Tissue Society Symposium
  • Place of Presentation: 神奈川
• [Presentation] The ion-diffusion barrier model at the node of Ranvier. From an extracellular ion diffusion analysis in Bral1-deficient mice (2009)
  • Author(s): 別宮洋子
  • Organizer: 第36回国際生理学会大会
  • Place of Presentation: 京都国際会館
• [Presentation] The nodal ECM barrier model in the CNS : From an extracellular ion diffusion analysis in Brall-deficient mice (2009)
  • Author(s): 別宮洋子
  • Organizer: Ninth Biennial Satellite Meeting of the ISN on Myelin Biology, Myelin Development Function and Related Diseases Conference
  • Place of Presentation: Gyeongju, S.Korea
• [Presentation] Btevican organizes hyalumnan-binding extracellular assemblies distinctively at the large diameter nodes of Ranvier in the CNS (2009)
  • Author(s): 別宮洋子
  • Organizer: Neuroscience 2009第32回日本神経科学会大会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] Deletion of the brain-specific link protein Bral-1 facilitates extracellular diffusion in the mouse corpus callosum. and E. Sykova (2008)
  • Author(s): Vargova L, Dmytrenko L, Vorisek I, Bekku Y, Oohashi T
  • Organizer: SfN 2008
  • Place of Presentation: Washington DC, USA
• [Presentation] Deletion of the brain-specific link protein Bral-1 facilitates extracellular diffusion in the mouse corpus callosum (2008)
  • Author(s): Vargova L, et al.
  • Organizer: Neuroscience 2008
  • Place of Presentation: Washington DC
• [Remarks] 岡山大学プレスリリース平成22年2月23日
• [Remarks] 山陽新聞掲載平成22年3月16日
  • URL: http://www.mbb-okayama.com/index.html
• [Remarks]
  • URL: http://www.mbb-okayama.com/index.html