Study on intractable otitis media caused by Nontypeable Haemophilus influenza (NTHi) biofilm formation

• Project/Area Number: 20791219
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: Wakayama Medical University
• Principal Investigator: IKEDA Yorihiko Wakayama Medical University, 医学部, 助教 (20423949)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: バイオフィルム / 急性中耳炎 / 難治化 / 中耳炎

Research Abstract

Nontypeable Haemophilus influenza (NTHi) has been shown to form biofilm in vivo. The biofilm is defined as a group of bacteria growing as a community in a self- produced polymeric matrix. Several studies show that the ability to form biolilms contributes the persistence of acute otitis media caused by NTHi. The biofilm formations and adherence of NTHi were evaluated by a 96-well pin-replicator assay with a confocal laser scanning microscopy. Bacteria in biofilms were highly resistant to antibiotics.

Research Products

• [Journal Article] Formation of biofilm by Haemophilus influenza isolated from pediatric intractable otitis media. (2009)
  • Author(s): Satomi Moriyama, Muneki Hotomi, Jun Shimada, Dewan S. Billal, Keiji Fujihara, Noboru Yamanaka
  • Journal Title: Auris Nasus Larynx 36 Pages: 525-531
• [Presentation] 小児急性中耳炎におけるインフルンザ菌のバイオフィルム形成と臨床経過の検討 (2009)
  • Author(s): 竹井慎, 他
  • Organizer: 第110回日本耳鼻咽喉科学会総会・学術講演会
  • Place of Presentation: 東京都
  • Year and Date: 2009-05-14
• [Presentation] Minimal biofilm eradication concentration of antibiotics against Haemophilus influenzae isolated from otitis media. (2009)
  • Author(s): Shin Takei, Muneki Hotomi, Satomi Moriyama, Dewan S. Billal, Yorihiko Ikeda, Noboru Yamanaka
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2009-05-06
• [Presentation] 急性中耳炎の難治化とインフルエンザ菌のバイオフィルム形成との関連 (2008)
  • Author(s): 竹井慎, 他
  • Organizer: 第56回日本化学療法学会西日本支部総会
  • Place of Presentation: 広島市
  • Year and Date: 2008-12-06
• [Presentation] 急性中耳炎の難治化とインフルエンザ菌のバイオフィルム形成との関連に関する研究 (2008)
  • Author(s): 竹井慎, 他
  • Organizer: 第38回日本耳鼻咽喉科感染症研究会、第32回日本医用エアロゾル研究会
  • Place of Presentation: 松江市
  • Year and Date: 2008-09-05
• [Presentation] 急性中耳炎の難治化とインフルエンザ菌のバイオフィルム形成との関連に関する研究 (2008)
  • Author(s): 竹井慎
  • Organizer: 第38回日本耳鼻咽喉科感染症研究会
  • Place of Presentation: 松江市