Protective effects of Channelrhodopsin-2 gene transfer on neuronal cell death

• Project/Area Number: 20791241
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Tohoku University
• Principal Investigator: ISAGO Hitomi Tohoku University, 国際高等研究教育機構, 技術補佐員 (30400451)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 眼生理学 / 神経科学 / 生理学 / 免疫組織化学

Research Abstract

We examined whether the transfer of ChR2 gene rescue the death of ganglion cells induced by the photoreceptor degeneration. We could clearly record the visually evoked potentials in both age of the ChR2 transferred-RCS rats though the amplitudes. These data showed that the function of retinal ganglion cells was kept after the photoreceptor degeneration.In the ChR2-expressing retina, the immunoreactivity (IM) of the p65-IM was observed in the retinal ganglion cell layer. It is known that the expression of p65 has a critical role in keeping the neuronal cell activities. These results indicated that the transfer of ChR2 gene could give some protective effect on the cell death of retinal ganglion cells following the photoreceptor degeneration.

Research Products

• [Journal Article] Channelrhodopsin-2 gene transduced into retinal ganglion cells restores functional vision in genetically blind rats. (2010)
  • Author(s): Tomita H, Sugano E, Isago H, Hiroi T, Wang Z, Ohta E, Tamai M
  • Journal Title: Experimental Eye Research 90 Pages: 429-436
  • Peer Reviewed
• [Journal Article] Channelrhodopsin-2 gene transduced into retinal ganglion cells restores functional vision in genetically blind rats (2010)
  • Author(s): Tomita H, Sugano E, Isago H, Hiroi T, Wang Z, Ohta E, Tamai M
  • Journal Title: Experimental Eye Research. 90(3) Pages: 429-436
  • Peer Reviewed
• [Journal Article] Channelrhodopsins provide a breakthrough insight into strategies for curing blindness. (2009)
  • Author(s): Tomita H, Sugano E, Isago H, Tamai M.
  • Journal Title: J. Genet. 88 Pages: 409-415
• [Journal Article] Visual Properties of Transgenic Rats Harboring the Channelrhodopsin-2 Gene regulated by the Thy-1.2 Promoter (2009)
  • Author(s): Tomita H, Sugano E, Fukazawa Y, Isago H, Sugiyama Y, Hiroi T, Ishizuka T, Mushiake H, Kato M, Hirabayashi M, Shigemoto R, Yawo H, Tamai M.
  • Journal Title: PLoS ONE 4(11)
  • Peer Reviewed
• [Journal Article] Channelrhodopsins provide a breakthrough insight into strategies for curing blindness (2009)
  • Author(s): Tomita H, Sugano E, Isago H, Tamai M.
  • Journal Title: Journal of genetics 88(4) Pages: 409-415
• [Journal Article] Brain-derived neurotrophic factor increases the phagocytic act ivity mediated through mitogen-activated protein kinase path way in cultured iris pigment epithelial cells (2008)
  • Author(s): Yoshida H., Tomita H., Sug ano E., Isago H., Ishiguro S.I., Tamai M
  • Journal Title: Cell Struct Funct. 33(1) : 21-6, 2008 33(1) Pages: 21-26
  • Peer Reviewed
• [Journal Article] チャネルロドプシン2遺伝子を用いた視機能再建の可能性 (2008)
  • Author(s): 富田浩史、菅野江里子、砂金ひとみ、廣井照、太田絵美、玉井信
  • Journal Title: Proceedings of the 34th Sensory Substi tution Symposium 34 Pages: 21-24
• [Presentation] Restore Vision Using Channelrhodopsin-2 : Character of Recovered Visual Responses in Channelrhodopsin-2 Transferred Royal College of Surgeons rats (2009)
  • Author(s): Tomita H, Sugano E, lsago H, Hiroi T, Tamai M
  • Organizer: Asia-ARVO(アジア眼科研究会議)
  • Place of Presentation: Hyderabad, India
  • Year and Date: 2009-01-18
• [Presentation] Restore Vision Using Channelrhodopsin-2 : Dissemination of AAV Vector in Channelrhodopsin-2 transferred rat (2009)
  • Author(s): Sugano E, Tomita H, lsago H, Hiroi T. Tamai M
  • Organizer: Asia-ARVO(アジア眼科研究会議)
  • Place of Presentation: Hyderabad, India
  • Year and Date: 2009-01-18
• [Presentation] Properties of recovered visual function in the Channelrhodopsin-2 transferred Royal College of Sugecns Rat. (2009)
  • Author(s): Tomita H. Sugano E. Isago H. Hiroi T. Yawo H. Tamai M.
  • Organizer: ARVO
  • Place of Presentation: Florida, USA
• [Presentation] Restore vision using Channelrhodopsin-2: Character of recovered visual responses in Channelrhodopsin-2 Transferred Royal College of Surgeons rats. (2009)
  • Author(s): Tomita H. Sugano E. Isago H. Hiroi T., Tamai M.
  • Organizer: Asia ARVO
  • Place of Presentation: Hyderabad, India
• [Presentation] Properties of recovered visual function in the Channelrhodopsin-2 transferred Royal College of Sugecns Rat (2009)
  • Author(s): Tomita H., Sugano E., Isago H., Hiroi T., Yawo H., Tamai, M.
  • Organizer: ARVO
  • Place of Presentation: Florida, USA
• [Presentation] Channelrodopsin-2による遺伝盲ラットの視機能再建-免疫学的検討- (2008)
  • Author(s): 菅野江里子, 富田浩史, 砂金ひとみ, 玉井信
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学
  • Year and Date: 2008-09-07
• [Presentation] チャネルロドプシン-2遺伝子を導入した老齢と若齢RCSラットの視覚誘発電位の違いについて (2008)
  • Author(s): 砂金ひとみ、富田浩史、菅野江里子、廣井照、玉井信
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学
  • Year and Date: 2008-09-07
• [Presentation] Channelrodopsin-2による遺伝盲ラットの視機能再建-免疫学的検討- (2008)
  • Author(s): 菅野江里子, 富田浩史, 砂金ひとみ, 玉井信
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡夫学七隈キャンパス
  • Year and Date: 2008-09-07
• [Presentation] チャネルロドプシン-2遺伝子を導入した老齢と若齢RCSラットの視覚誘発電位の違いについて (2008)
  • Author(s): 砂金ひとみ、富田浩史、菅野江里子、廣井照、玉井信
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学七隈キャンパス
  • Year and Date: 2008-09-07
• [Presentation] 蛍光タンパク質の付加によるChannelrodopsin-2光感受性の改善 (2008)
  • Author(s): 富田浩史, 菅野江里子, 砂金ひとみ, 宮崎憲一, 八尾寛, 玉井信
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学七隈キャンパス
  • Year and Date: 2008-09-05
• [Remarks]
  • URL: http://www.visual-neuroscience.med.tohoku.ac.jp/
• [Remarks]
  • URL: http://www.visual-neuroscience.med.tohoku.ac.jp/
• [Remarks]
  • URL: http://www.visualneuroscience.med.tohoku.ac.jp/index.Html