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A study on function and characteristic analysis and application of the cornea and the conjunctiva epithelium stem cell for regenerative medicine realization

Research Project

Project/Area Number 20791247
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Ophthalmology
Research InstitutionThe University of Tokyo

Principal Investigator

YOKOO Seiichi  The University of Tokyo, 医学部・附属病院, 特任研究員 (20345052)

Co-Investigator(Renkei-kenkyūsha) YAMAGAMI Satoru  東京女子医科大学, 医学部, 准教授 (10220245)
Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords再生医療 / 角膜 / 上皮 / 幹細胞 / 体性幹細胞 / 幹細胞の単離 / 無血清培養 / 無フィーダー / 成体幹細胞 / 無血清 / 多分化能 / 組織幹細胞
Research Abstract

I discovered the new characteristic of the stem cell and developed the extremely cheap easy isolation method to overcome and discovered a corneal epithelial stem cell than adult human cornea limbus. Furthermore, I developed the corneal epithelium cell sheet which I established the serum-free culture method and could use for regenerative medicine.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (44 results)

All 2010 2009 2008 2007 2005

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (26 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Immunological mechanisms of corneal allografts reconstituted from cultured allogeneic endothelial cells in an immune-privileged site.2009

    • Author(s)
      Hayashi T, Yamagami S, Tanaka K, Yokoo S, Usui T, Amano S, Mizuki N
    • Journal Title

      Invest Ophthalmol Vis Sci. 50巻

      Pages: 3151-8

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selective Isolation of Young Cells from Human Corneal Endothelium by the Sphere-Forming Assay.2009

    • Author(s)
      Mimura T, Yamagami S, Yokoo S, Usui T, Amano S
    • Journal Title

      Tissue Eng Part C Methods

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selective Isolation of Young Cells from Human Corneal Endothelium by the Sphere-Forming Assay2009

    • Author(s)
      Mimura T, Yamagami S, Yokoo S, Usui T, Amano S.
    • Journal Title

      Tissue Eng Part C Methods online

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunological mechanisms of corneal allografts reconstituted from cultured allogeneic endothelial cells in an immune-privileged site2009

    • Author(s)
      Hayashi T, Yamagami S, Tanaka K, Yokoo S, Usui T, Amano S, Mizuki N.
    • Journal Title

      Invest Ophthalmol Vis Sci (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel isolation technique of progenitor cells in human corneal epithelium using non-tissue culture dishes.2008

    • Author(s)
      Yokoo S, Yamagami S, Shimada T, Usui T, Sato TA, Amano S, Araie M, Hamuro J.
    • Journal Title

      Stem Cells. 26巻

      Pages: 1743-1748

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human corneal epithelial equivalents for ocular surface reconstruction in a complete serum-free culture system without unknown factors.2008

    • Author(s)
      Yokoo S, Yamagami S, Usui T, Amano S, Araie M.
    • Journal Title

      Invest Ophthalmol Vis Sci. 49巻

      Pages: 2438-43

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Decellularizing corneal stroma using N2 gas.2008

    • Author(s)
      Amano S, Shimomura N, Yokoo S, Araki-Sasaki K, Yamagami S
    • Journal Title

      Mol Vis. 14巻

      Pages: 878-82

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A mouse model of allogeneic corneal endothelial cell transplantation.2008

    • Author(s)
      Hayashi T, Yamagami S, Tanaka K, Yokoo S, Usui T, Amano S, Mizuki N
    • Journal Title

      Cornea. 27巻

      Pages: 699-705

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of corneal neovascularization by blocking the angiotensin II type 1 receptor.2008

    • Author(s)
      Usui T, Sugisaki K, Iriyama A, Yokoo S, Yamagami S, Nagai N, Ishida S, Amano S
    • Journal Title

      Invest Ophthalmol Vis Sci 49巻

      Pages: 4370-6

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article]2008

    • Author(s)
      Mimura T, Amano S, Yokoo S, Uchida S, Yamagami S, Usui T, Kimura Y, Tabata Y
    • Journal Title

      Mol Vis. 14巻

      Pages: 1819-28

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tissue engineering of corneal stroma with rabbit fibroblast precursors and gelatin hydrogels2008

    • Author(s)
      Mimura T, Amano S, Yokoo S, Uchida S, Yamagami S, Usui,T, Kimura Y, Tabata Y.
    • Journal Title

      Mol Vis 14

      Pages: 1819-28

    • NAID

      120001851020

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of corneal neovaseularization by blocking the angiotensin II type 1 receptor2008

    • Author(s)
      Usui T, Sugisaki K, Iriyama A, Yokoo S, Yamagami S, N agai N, Ishida S, Amano S.
    • Journal Title

      Invest Ophthalmol Vis Sci 49

      Pages: 4370-6

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A mouse model of allogeneic corneal endothelial cell transplantation2008

    • Author(s)
      Hayashi T, Yamagami S, Tanaka K ; Yokoo S, Usui T, Amano S ; Mizuki N.
    • Journal Title

      Cornea 27

      Pages: 699-705

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human corneal epithelial equivalents for ocular surface reconstruction in a complete serum-free culture system without unknown factors2008

    • Author(s)
      Yokoo S, Yamagami S, Usui T , Amano S, Araie M.
    • Journal Title

      Invest Ophthalmol Vis Sci 49

      Pages: 2438-43

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Decellularizing corneal stroma using N2 gas2008

    • Author(s)
      Amano S, Shimomura N, Yokoo S, Araki?Sasaki K, Yamagami S.
    • Journal Title

      Mol Vis 14

      Pages: 878-82

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel isolation technique of progenitor cells in human corneal epithelium using non-tissue culture dishes2008

    • Author(s)
      Yokoo S, Yamagami S, Shimaoa T, Usui T, Sato TA, Amano S, Araie M, Hamuro J.
    • Journal Title

      Stem Cells 26

      Pages: 1743-1748

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] ヒト培養角膜上皮細胞シートにおける塩化ベンザルコニウムの影響2010

    • Author(s)
      大道幸子、臼井智彦、横尾誠一、新家眞、天野史郎
    • Organizer
      角膜カンファランス
    • Place of Presentation
      仙台国際センター(仙台市)
    • Year and Date
      2010-02-11
    • Related Report
      2009 Final Research Report
  • [Presentation] kgf添加無フィーダー細胞・無血清角膜上皮シートのサイトケラチン15の発現2010

    • Author(s)
      横尾誠一、山上聡、天野史郎、新家眞
    • Organizer
      角膜カンファランス
    • Place of Presentation
      仙台国際センター(仙台市)
    • Year and Date
      2010-02-11
    • Related Report
      2009 Final Research Report
  • [Presentation] nbc1 w516xホモノックインマウスにおける角膜所見2010

    • Author(s)
      臼井智彦、本田紀彦、横尾誠一、新家眞、天野史郎、林石化、関常司
    • Organizer
      角膜カンファランス
    • Place of Presentation
      仙台国際センター(仙台市)
    • Year and Date
      2010-02-11
    • Related Report
      2009 Final Research Report
  • [Presentation] 角膜血管新生における白血病抑制因子の関与2010

    • Author(s)
      杉崎顕史、臼井智彦、本田紀彦、横尾誠一、天野史郎
    • Organizer
      角膜カンファランス
    • Place of Presentation
      仙台国際センター(仙台市)
    • Year and Date
      2010-02-11
    • Related Report
      2009 Final Research Report
  • [Presentation] ヒト培養角膜上皮細胞シートにおける塩化ベンザルコニウムの影響2010

    • Author(s)
      大道幸子、臼井智彦、横尾誠一、新家眞、天野史郎
    • Organizer
      角膜カンファランス
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2010-02-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] KGF添加無フィーダー細胞・無血清角膜上皮シートのサイトケラチン15の発現2010

    • Author(s)
      横尾誠一、山上聡、天野史郎、新家眞
    • Organizer
      角膜カンファランス
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2010-02-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] NBCl W516Xホモノックインマウスにおける角膜所見2010

    • Author(s)
      臼井智彦、本田紀彦、横尾誠一、新家眞、天野史郎、林石化、関常司
    • Organizer
      角膜カンファランス
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2010-02-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] 角膜血管新生における白血病抑制因子の関与2010

    • Author(s)
      杉崎顕史、臼井智彦、本田紀彦、横尾誠一、天野史郎
    • Organizer
      角膜カンファランス
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2010-02-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] 成人結膜上皮からの組織幹細胞の探索2009

    • Author(s)
      横尾誠一、臼井智彦、天野史郎、山上聡
    • Organizer
      日本組織培養学会
    • Place of Presentation
      獨協医科大学(栃木県下都賀郡)
    • Year and Date
      2009-05-19
    • Related Report
      2009 Final Research Report
  • [Presentation] 成人結膜上皮からの組織幹細胞の探索(ワークショップ)2009

    • Author(s)
      横尾誠一、臼井智彦、天野史郎、山上聡
    • Organizer
      日本組織培養学会
    • Place of Presentation
      獨協医科大学
    • Year and Date
      2009-05-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒト角結膜の組織幹細胞・前駆細胞の特徴2009

    • Author(s)
      横尾誠一
    • Organizer
      日本眼科学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-04-17
    • Related Report
      2009 Final Research Report
  • [Presentation] 無フィーダー細胞・無血清培養法による移植用上皮シートの作成と評価2009

    • Author(s)
      横尾誠一、臼井智彦、本田紀彦、天野史郎、山上聡
    • Organizer
      日本眼科学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2009-04-17
    • Related Report
      2009 Final Research Report
  • [Presentation] ヒト角膜輪部上皮前駆細胞の分化能の検討2009

    • Author(s)
      横尾誠一、臼井智彦、三村達哉、杉崎顕史、天野史郎、山上聡
    • Organizer
      日本眼科学会
    • Place of Presentation
      東京国際フォーラム(東京)
    • Year and Date
      2009-04-17
    • Related Report
      2009 Final Research Report
  • [Presentation] 可溶性VEGFR2による角膜移植後の炎症細胞の浸潤抑制・リンパ管新生阻害効果2009

    • Author(s)
      林孝彦、臼井智彦、横尾誠一、Romulo Albuquerque, Jayakrishna Ambati, Balamurali Ambati, 天野史郎、水木信久、山上聡
    • Organizer
      日本眼科学会
    • Place of Presentation
      東京国際フォーラム(東京)
    • Year and Date
      2009-04-17
    • Related Report
      2009 Final Research Report
  • [Presentation] ヒト角膜輪部上皮前駆細胞の分化能の検討2009

    • Author(s)
      横尾誠一、臼井智彦、三村達哉、杉崎顕史、天野史郎、山上聡
    • Organizer
      日本眼科学会
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2009-04-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] 可溶性VEGFR2による角膜移植後の炎症細胞の浸潤抑制・リンパ管新生阻害効果2009

    • Author(s)
      林孝彦、臼井智彦、横尾誠一、Romulo Albuquerque, Jayakrishna Ambati, Balamurali Ambati, 天野史郎、水木信久、山上聡
    • Organizer
      日本眼科学会
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2009-04-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒト角結膜の組織幹細胞・前駆細胞の特徴(シンポジウム)2009

    • Author(s)
      横尾誠一
    • Organizer
      日本眼科学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2009-04-17
    • Related Report
      2008 Annual Research Report
  • [Presentation] 無フィーダー細胞・無血清培養法による移植用上皮シートの作成と評価(口演)2009

    • Author(s)
      横尾誠一、臼井智彦、本田紀彦、天野史郎、山上聡
    • Organizer
      日本眼科学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2009-04-17
    • Related Report
      2008 Annual Research Report
  • [Presentation] マウス角膜血管新生モデルにおけるアンジオポエチン様因子2の発現2009

    • Author(s)
      臼井智彦、本田紀彦、横尾誠一、三村達哉、天野史郎、尾池雄一
    • Organizer
      角膜カンファランス・日本角膜移植学会
    • Place of Presentation
      ザ・リッツ・カールトン大阪(大阪市)
    • Year and Date
      2009-02-20
    • Related Report
      2009 Final Research Report
  • [Presentation] 可溶性VEGFR2を用いた角膜移植後感作の抑制2009

    • Author(s)
      林孝彦、臼井智彦、横尾誠一、Romulo Albuquerque、Jayakrishna Ambati、Balamurali Ambati、天野史郎、水木信久、山上聡
    • Organizer
      角膜カンファランス・日本角膜移植学会
    • Place of Presentation
      ザ・リッツ・カールトン大阪(大阪市)
    • Year and Date
      2009-02-20
    • Related Report
      2009 Final Research Report
  • [Presentation] マウス角膜血管新生モデルにおけるアンジオポエチン様因子2の発現(学術展示)2009

    • Author(s)
      臼井智彦、本田紀彦、横尾誠一、三村達哉、天野史郎、尾池雄一
    • Organizer
      角膜カンファランス・日本角膜移植学会
    • Place of Presentation
      ザ・リッツ・カールトン大阪(大阪市)
    • Year and Date
      2009-02-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] 可溶性VEGFR2を用いた角膜移植後感作の抑制(学術展示)2009

    • Author(s)
      林孝彦、臼井智彦、横尾誠一、Romulo Albuquerque、Jayakris hna Ambati、Balamurali Ambati、天野史郎、水木信久、山上聡
    • Organizer
      角膜カンファランス・日本角膜移植学会
    • Place of Presentation
      ザ・リッツ・カールトン大阪(大阪市)一
    • Year and Date
      2009-02-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] 成人角膜上皮幹細胞を用いた無血清角膜上皮シートの開発2008

    • Author(s)
      横尾誠一、臼井智彦、小野恭子、天野史郎、山上聡
    • Organizer
      眼科再生医療研究会), 日本臨床眼科学会
    • Place of Presentation
      東京国際フォーラム(東京)
    • Year and Date
      2008-10-23
    • Related Report
      2009 Final Research Report
  • [Presentation] 成人角膜上皮幹細胞を用いた無血清角膜丑皮シートの開発(眼科再生医療研究会)2008

    • Author(s)
      横尾誠一、臼井智彦、小野恭子、天野史郎、山上聡
    • Organizer
      日本臨床眼科学会
    • Place of Presentation
      東京国際フォーラム(東京)
    • Year and Date
      2008-10-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] 成人角膜上皮幹細胞を用いた移植用無血清角膜上皮シートの開発2008

    • Author(s)
      横尾誠一、臼井智彦、天野史郎、山上聡
    • Organizer
      日本組織培養学会
    • Place of Presentation
      文部科学省研究交流センター(つくば市)
    • Year and Date
      2008-05-20
    • Related Report
      2009 Final Research Report
  • [Presentation] 成人角膜上皮幹細胞を用いた移植用無血清角膜上皮シートの開発(口演)2008

    • Author(s)
      横尾誠一、臼井智彦、天野史郎、山上聡
    • Organizer
      日本組織培養学会
    • Place of Presentation
      文部科学省研究交流センター(つくば市)
    • Year and Date
      2008-05-20
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] METHOD FOR ISOLATION OF CELL, SERUM-FREE CULTURE MEDIUM FOR CELL, AND METHOD FOR CULTURE OF CELL (細胞の単離方法、細胞用無血清培養培地および細胞の培養方法)2007

    • Inventor(s)
      YOKOO, Seiichi、YAMAGAMI, Satoru、
    • Industrial Property Rights Holder
      MITSUBISHI TANABE PHARMA CORPORATION, YOKOO, Seiichi、YAMAGAMI, Satoru
    • Filing Date
      2007-07-13
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 生細胞分離用遠心分離管2005

    • Inventor(s)
      横尾誠一、小野恭子、山上聡、田中速雄
    • Industrial Property Rights Holder
      横尾誠一、住友ベークライト株式会社
    • Patent Publication Number
      2007-166977
    • Filing Date
      2005-12-22
    • Related Report
      2009 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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