Analysis of molecular mechanisms in endogenous oxidative stress response and inhibition of retinal degeneration

• Project/Area Number: 20791251
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Shimane University
• Principal Investigator: TANITO Masaki Shimane University, 医学部, 講師 (30284037)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 網膜光傷害 / 酸化ストレス / 網膜変性 / 脂質過酸化 / Nrf-2 / 網膜光障害

Research Abstract

Protein expressions were analyzed in retinal samples from dark- and bright light-reared rats by using proteomics techniques. The results suggested that groups of proteins from several functional categories were involved in retinal protection in in vivo. Retinal levels of fatty acid were changed by a combination use of transgenic mice carrying n-3 fatty acid desaturase and modified fatty acid diet. The results of suggested that lower ratio of n6/n3 fatty acid in retina was related to the high susceptibility of retinal damage induced by intense light exposure.

Research Products

• [Journal Article] 「スルフォラファンとNrf2」眼と機能性食品 (2009)
  • Author(s): 谷戸正樹
  • Journal Title: Functional Food 3(1) Pages: 14-20
• [Journal Article] High levels of retinal membrane docosahexaenoic acid increase susceptibility to stress-induced degeneration. (2009)
  • Author(s): Tanito M, et al.
  • Journal Title: Journal of Lipid Research 50(5) Pages: 807-819
  • Peer Reviewed
• [Journal Article] Topography of retinal damage in light-exposed albino rats. (2008)
  • Author(s): Tanito M, Kaidzu S, Ohira A, Anderson RE
  • Journal Title: Experimental Eye Research 87(3) Pages: 292-295
• [Journal Article] Topography of retinal damage in light-exposed albino rats (2008)
  • Author(s): Tanito M, et al.
  • Journal Title: Experimental Eye Research 87 Pages: 292-295
  • Peer Reviewed
• [Journal Article] High levels of retina membrane docosahexaenoic acid increase susceptibility to stress induced degeneration.
  • Author(s): Tanito M, Brush RS, Elliott MH, Wicker LD, Henry KR, Anderson RE
  • Journal Title: Journal of Lipid Research 50(5) Pages: 807-819
• [Journal Article] 眼と内科疾患、3. 酸化ストレスと眼
  • Author(s): 谷戸正樹
  • Journal Title: Pharma Medica 26(9) Pages: 19-23
• [Presentation] 視細胞外節のドコサヘキサエン酸量と光による網膜傷害度の関係 (2009)
  • Author(s): 谷戸正樹, Brush RS, Elliott MH, Wicker LD, Henry KR, Anderson RE
  • Organizer: 第54回山陰眼科集談会
  • Place of Presentation: 出雲市
  • Year and Date: 2009-06-07
• [Presentation] 光照射したラット網膜における傷害の分布 (2009)
  • Author(s): 海津幸子, 谷戸正樹, 大平明弘, Anderson RE
  • Organizer: 第54回山陰眼科集談会
  • Place of Presentation: 出雲市
  • Year and Date: 2009-06-07
• [Presentation] シンポジウム2「感覚器とアンチエイジング」加齢黄斑変性と酸化ストレスマーカー (2009)
  • Author(s): 谷戸正樹
  • Organizer: 第9回抗加齢医学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-05-28
• [Presentation] シンポジウム「ストレス応答と眼疾患」網膜におけるNrf2~光ストレス応答機構 (2009)
  • Author(s): 谷戸正樹
  • Organizer: 第2回東京アンチエイジングアカデミー
  • Place of Presentation: 東京
  • Year and Date: 2009-05-27
• [Presentation] Symposium III Basic Science, Hormesis paradigm in a therapeutic strategy against retinal (2009)
  • Author(s): Tanito M
  • Organizer: neurodegeneration Glaucoma Summer Camp 2009 at Lake Biwa
  • Place of Presentation: Ohtsu, Shiga
• [Presentation] 網膜における光ストレス応答機構 (2009)
  • Author(s): 谷戸正樹
  • Organizer: 第31回日本光医学光生物学会
  • Place of Presentation: 大阪市
• [Presentation] 網膜内ドコサヘキサエン酸量と光による網膜障害の関係 (2009)
  • Author(s): 谷戸正樹, Brush RS, Elliott MH, Wicker LD, Henry KR, Anderson RE
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京
• [Presentation] 可視光照射によるラット網膜障害の波長依存性 (2009)
  • Author(s): 海津幸子, 奥野勉, 谷戸正樹, 大平明弘
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京
• [Presentation] シンポジウム「分子レベルで見た網膜変性の酸化ストレス」明環境順応による網膜防御とその分子機構 (2008)
  • Author(s): 谷戸正樹
  • Organizer: 第112回日本眼科学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2008-04-17
• [Presentation] 明環境順応による網膜防御とその分子機構 (2008)
  • Author(s): 谷戸正樹
  • Organizer: 第112回日本眼科学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2008-04-17
• [Presentation] Correlation between tissue docosahexaenoic acid levels and susceptibility against light-induced retinal degeneration. (2008)
  • Author(s): Tanito M, Elliott MH, Brush RS, Wicker LD, Henry KR, Anderson RE
  • Organizer: XIIIth International Symposium on Retinal Degeneration
  • Place of Presentation: Emeishan, China
• [Presentation] Comparison of the susceptibility of fat-1 and wild type mice fed an n-3 polyunsaturated fatty acid deficient diet to light-induced retinal degeneration. (2008)
  • Author(s): Tanito M, Elliott ME, Brush RS, Wicker LD, Henry KR, Anderson RE
  • Organizer: ARVO 2008 Annual Meeting
  • Place of Presentation: Fort Lauderdale, FL