Localization and quantitative analysis of retinal protein by lasermicrodissection

Research Project

Project/Area Number	20791252
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Ophthalmology
Research Institution	Shimane University
Principal Investigator	KAIDZU Sachiko Shimane University, 医学部, 助教 (00325052)
Project Period (FY)	2008 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords	眼組織学 / 網膜光傷害 / レーザーマイクロダイセクション / 網膜光障害 / 酸化ストレス / 抗酸化酵素 / プロテオミクス
Research Abstract	To study the change of antioxidants in retinal layers, we used lasemicrodissection. Light damaged rat retina were divided into 4 segments ; retinal pigment epithelium, rod outer segments～outer plexiform layer, inner nuclear layer～inner plexiform layer, ganglion cell layer～nerve fiber layer. Antioxidants were increased in each layer, particularly in rod outer segments～outer plexiform layer, and the change of localization and expression were almost similar to the results by immunohisochemical method. Lasermicrodissection is useful to study the localization and quantitative change of protein at cellular levels.

Report

(4 results)