Normal tension glaucoma model: Characterization of retinal neuron specific BDNF and cfos knockout

• Project/Area Number: 20791257
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Ehime University
• Principal Investigator: KOBAYASHI Takeshi Ehime University, 大学院・医学系研究科, 寄附講座助教 (70380285)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 緑内障 / 正常眼圧 / 網膜 / 脳由来神経栄養因子 / cfos / ノックアウトマウス / 綱膜 / BDNF / 組織特異的 / シグナルトランスダクション

Research Abstract

Retinal neuron specific (Brain-derived neurotrophic factor) BDNF and cfos knockout were studied to characterize the phenotypical change in retinal ganglion cells (RGC). In BDNF knockout, RGC gradually decrease from peripheral potion of retina by aging. Reduction ratio of RGC in cfos knockout was higher than that of RGC in BDNF knockout. Neural retina of cfos knockout did not express mBdnf. It suggest cfos is upstream of BDNF and other neuroprotective factor in the mouse retina. For preventing normal tension glaucoma, neuroprotective transcription factor should be analyzed.

Research Products

• [Journal Article] Rapid detection of Acanthamoeba cysts in frozen sections of corneal scrapings with Fungiflora Y (2009)
  • Author(s): Shiraishi A, Kobayashi T, Hara Y, Yamaguchi M, Uno T, Ohashi Y
  • Journal Title: Br J Ophthalmol 93 Pages: 1563-1565
  • Peer Reviewed
• [Journal Article] New technique for culturing corneal epithelial cells of normal mice (2009)
  • Author(s): Kobayashi T, Yoshioka R, Shiraishi A, Ohashi Y
  • Journal Title: Molecular Vision. 15 Pages: 1589-1593
  • Peer Reviewed
• [Journal Article] Alteration of TLR3 pathways by glucocorticoids may be responsible for immunosusceptibility of human corneal epithelial cells to viral infections (2009)
  • Author(s): Hara Y, Shiraishi A, Kobayashi T, Kadota Y, Shirakata Y, Hashimoto K, Ohashi Y.
  • Journal Title: Molecular Vision. 15 Pages: 7-948
  • Peer Reviewed
• [Journal Article] Rapid detection of Acanthamoeba cysts in frozen sections of corneal scra pings with Fungiflora Y (2009)
  • Author(s): Shiraishi A, Kobayashi T, Hara Y, Yamaguchi M, Uno T, Ohashi Y
  • Journal Title: Br J Ophthalmol. 93 Pages: 1563-1565
  • Peer Reviewed
• [Journal Article] Alteration of TLR3 pathways by glucocorticoids may be responsible for immunosusceptibility of human corneal epithelial cells to viral infections (2009)
  • Author(s): Hara Y, Shiraishi A, Kobayashi T, Kadota Y, Shirakata Y, Hashimoto K, Ohashi Y
  • Journal Title: Moleculaz Vision. 15 Pages: 937-948
  • Peer Reviewed
• [Journal Article] High Occurrence Rate of Tetracycline (TC)-Resistant Bacteria and TC Resistance Genes Relates to Microbial Diversity in Sediment of Mekong River Main Waterway (2008)
  • Author(s): Suzuki S, Kobayashi T, Suehiro F, Tuyen BC, Tana TS
  • Journal Title: Microbes and Environments. 23(2) Pages: 149-152
  • NAID: 110006684366
  • Peer Reviewed
• [Presentation] 凍結保存細胞によるヒト培養角膜上皮シートの作製 (2010)
  • Author(s): 小林剛、白石敦、楊旅軍、白方裕司、橋本公二、大橋裕一
  • Organizer: 第34回角膜カンファランス
  • Place of Presentation: 仙台
• [Presentation] コラーゲンゲルを用いた角膜上皮培養系における上皮-実質間相互作用の検討 (2009)
  • Author(s): 小林剛、白石敦、楊旅軍、白方裕司、橋本公二、大橋裕一
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京
• [Presentation] コラーゲンゲルを用いた三次元上皮培養におけるゲル内実質細胞の増殖因子発現パターン (2009)
  • Author(s): 小林剛、白石敦、楊旅軍、白方裕司、橋本公二、大橋裕一
  • Organizer: 第33回角膜カンファランス
  • Place of Presentation: 大阪
• [Presentation] コラーゲンゲルを用いた培養ヒト角膜上皮シートの作製とその評価 (2008)
  • Author(s): 小林剛、白石敦、原祐子、門田裕子、楊旅軍、白方裕司、橋本公二、大橋裕一
  • Organizer: 第62回日本臨床眼科学会
  • Place of Presentation: 東京