Primary culture of mice non-pigmented ciliary epithelium and establishment of the screening system of anti-glaucoma medications by using mice non-pigmented ciliary epithelium in vitro.

• Project/Area Number: 20791273
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Keio University
• Principal Investigator: YUKI Kenya Keio University, 医学部, 研究員(非常勤) (00365347)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 無色素上皮 / 緑内障 / 毛様体 / アクアポリン / 眼圧 / 眼 / 毛様体無色素上皮 / アクアポーリン

Research Abstract

We tried to cultivate non-pigmented ciliary epithelial cells by explant of ciliary body. However, only pigmented ciliary cells were obtained. In the experiment, we observed sphere formation of GFP-positive cells from ciliary body of P0cre-GFP mice. This showed that neural stem cell from neural crest presents in the ciliary body of mice.