Proteomic analysis of the disease specific autoantibodies in Behcet's disease

• Project/Area Number: 20791279
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Tokyo Medical University
• Principal Investigator: OKUNUKI Yoko Tokyo Medical University, 医学部, 助教 (40459508)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: プロテオミクス / 内因性ぶどう膜炎 / 自己抗原 / ぶどう膜炎 / 自己免疫 / 網膜抗原

Research Abstract

A proteomic approach combined with western blotting was applied to detect multiple retinal autoantigens in endogenous uveitis. As results, Esterase D and brain type creatine kinase were defined as retinal autoantigens in endogenous uveitis, including Behcet's disease, sarcoidosis, and Vogt-Koyanagi-Harada disease. There was no disease specificity in the two newly defined autoantigens.

Research Products

• [Journal Article] Suppression of experimental autoimmue uveitis by angiotensin II type l receptor blocker telmisartan. (2009)
  • Author(s): Okunuki Y, et al
  • Journal Title: Investigative Ophthalmology and Visual Science 50 Pages: 2255-2261
  • Peer Reviewed
• [Journal Article] Suppression of experimental autoimmume uveoretinitis by regulatory dendritic cells in mice. (2009)
  • Author(s): Usui Y, Okunuki Y, et al
  • Journal Title: Archives of Ophthalmology 127 Pages: 514-519
  • Peer Reviewed
• [Journal Article] 制御性樹状細胞を用いた実験的自己免疫性ぶどう膜網膜炎の抑制 (2009)
  • Author(s): 臼井嘉彦、奥貫陽子, ら
  • Journal Title: 日本眼科学会雑誌 113 Pages: 819-819
• [Journal Article] Human iris pigment epithelial cels suppress T-cell activation viadireact cell contact. (2009)
  • Author(s): Hattori T, Okunuki Y, et al
  • Journal Title: Experimental Eye Research Pages: 358-64
  • Peer Reviewed
• [Journal Article] Functional and morphological changes in the eyes of Bechcet's patients with uveitis. (2009)
  • Author(s): Takeuchi M, Okunuki Y, et al
  • Journal Title: Acta Ophthalmologica Jun 1
  • Peer Reviewed
• [Journal Article] Correlation of vascular endothelial growth factor with chemokiens in the vitreous in diabetic retinopathy. (2009)
  • Author(s): Okunuki Y, et al
  • Journal Title: Retina 30 Pages: 339-344
  • Peer Reviewed
• [Journal Article] Immune responses to interphotoreceptor retinoid-binding protein and S-antigen in Behcet's patients with uveitis. (2009)
  • Author(s): Takeuchi M, Okunuki Y, et al
  • Journal Title: Investigative Ophthalmology and Visual Science Jan 20
  • Peer Reviewed
• [Journal Article] Effect of dioxin on vascular endothelial growth factor (VEGF) production in the retina associated with choroidal neovascularization. (2009)
  • Author(s): Takeuci A, Okunuki Y, et al
  • Journal Title: Investigative Ophthalmology and Visual Science 50 Pages: 3410-4316
  • Peer Reviewed
• [Journal Article] Dioxin promotes vascular endothelial growth factor production in the retina and enhances choroidal neovascularization (2009)
  • Author(s): Takeuchi A, Okunuki Y, et.al.
  • Journal Title: Investigative Opththalmology and Visual Sicence (In press)
  • Peer Reviewed
• [Journal Article] Angiotensin 2 type 1 receptor blocker telmisartan suppress experimenta ; autoimmune uveitis (2009)
  • Author(s): Okunuki Y, et.al.
  • Journal Title: Investigative Opththalmology and Visual Sicence (In press)
  • Peer Reviewed
• [Journal Article] Proteomic surveillance of retinal autoantigens in endogenous uveitis:implication of Esterase D and brain-type creatine kinase as novel autoantigens. (2008)
  • Author(s): Okunuki Y, et al
  • Journal Title: Molecular Vision 12巻 Pages: 1094-1104
  • Peer Reviewed
• [Journal Article] Proteomic surveillance of retinal autoantigens in endogeous uveitis : implication of esteraseD and brain-type creatine kinase as novel autoantigens (2008)
  • Author(s): Okunuki Y., et.al.
  • Journal Title: Molecular Vision 14 Pages: 1094-104
  • Peer Reviewed
• [Journal Article] Increased levels of monokine induced by interferon-gamma (Mig) in the vitreous of patients of diabetic retinopathy (2008)
  • Author(s): Okunuki Y., et.al.
  • Journal Title: Diabetic Medicine 25 Pages: 875-7
  • Peer Reviewed
• [Presentation] Analysis of multiple chemokines and growth factors in the vitreous af patients with branch retinal vein occlusion. (2009)
  • Author(s): Okunuki Y, et al
  • Organizer: Asssociation for research and in vision and ophthalmology
  • Place of Presentation: アメリカフロリダ州
• [Presentation] Vogt-小柳原田病患者の末梢血単核球サイトカイン産生とステロイド治療効果との関連 (2009)
  • Author(s): 奥貫陽子, ら
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京
• [Presentation] 先発眼から長期経過後に瞭眼に発症した急性網膜壊死の4例 (2009)
  • Author(s): 奥貫陽子, ら
  • Organizer: 第43回眼炎症学会
  • Place of Presentation: 大阪
• [Presentation] 網膜血管に異常を認めたAccute outer retinopathyの1例 (2009)
  • Author(s): 奥貫陽子, ら
  • Organizer: 第48回網膜硝子体学会総会
  • Place of Presentation: 名古屋
• [Presentation] Cytokine analysis to estimate the effect of corticosteroid in Vogt-Koyanagi-Harada disease patients (2009)
  • Author(s): Okunuki Y, et.al.
  • Organizer: 6^<th> international workshop on Vogt-Koyanagi-Harada Disease and Sympathetic Ophthalmia
  • Place of Presentation: シンガポール
• [Presentation] 実験的自己免疫性ぶどう膜網膜炎における肥満細胞の役割 (2008)
  • Author(s): 奥貫陽子, ら
  • Organizer: 第161回東京医科大学医学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-07
• [Presentation] 免疫原性を有する網膜局在タンパクのプロテオーム解析 (2008)
  • Author(s): 奥貫陽子、竹内大, ら
  • Organizer: 東京医科大学ハイテクリサーチセンター最終研究成果
  • Place of Presentation: 公開シンポジウム
  • Year and Date: 2008-01-21
• [Presentation] 網膜静脈分子閉塞症における硝子体中ケモカイン・サイトカインの解析 (2008)
  • Author(s): 奥貫陽子, ら
  • Organizer: 第112回日本眼科学会
  • Place of Presentation: 横浜
• [Presentation] Suppression of experimental autoimmne uveoretinitis by blocking angiotensin 2 type 1 receptor (2008)
  • Author(s): Okunuki Y, et.al.
  • Organizer: Association for Reseach in Vision and Ophthalmology
  • Place of Presentation: アメリカ、フロリダ州
• [Presentation] 網膜中心静脈閉塞症様所見を呈した非肉芽腫性虹彩毛様体炎の2例 (2008)
  • Author(s): 奥貫陽子, ら
  • Organizer: 第62回日本臨床眼科学会
  • Place of Presentation: 東京