Simultaneous observation of platelets and endothelial progenitor cells in corneal neovascularization

• Project/Area Number: 20791294
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Kyoto University
• Principal Investigator: YAMASHIRO Kenji Kyoto University, 医学研究科, 助教 (80399617)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 眼細胞生物学 / 角膜新生血管 / 血小板 / 血管内皮前駆細胞

Research Abstract

We observed platelets and endothelial progenitor cell simultaneously in corneal neovascularization. Fluorescence-labeled cells can be observed using Heidelberg Retina Angiography(HRA2)and stereomicroscope. Both cells roll along and adhere to vessel wall, which can be suppressed with anti-selectin antibody.

Research Products

• [Journal Article] Complications of Age-Related Macular Degeneration Prevention Trial (CAPT) Research Group (2010)
  • Author(s): Chen W, Stambolian D, Edwards AO, Branham KE, Othman M, Jakobsdottir J, Tosakulwong N, Pericak-Vance MA, Campochiaro PA, Klein ML, Tan PL, Conley YP, Kanda A, Kopplin L, Li Y, Augustaitis KJ, Karoukis AJ, Scott WK, Agarwal A, Kovach JL, Schwartz SG, Postel EA, Brooks M, Baratz KH, Brown WL
  • Journal Title: Proc Natl Acad Sci USA (in press)
  • Peer Reviewed
• [Journal Article] Thickness of photoreceptor layers in polypoidal choroidal vasculopathy and central serous chorioretinopathy. (2010)
  • Author(s): Ooto S, Tsujikawa A, Mori S, Tamura H, Yamashiro K, Yoshimura N
  • Journal Title: Graefes Arch Clin Exp Ophthalmol (in press)
  • NAID: 120002514711
  • Peer Reviewed
• [Journal Article] Restoration of outer segments of foveal photoreceptors after resolution of central serous chorioretinopathy. (2010)
  • Author(s): Ojima Y, Tsujikawa A, Yamashiro K, Ooto S, Tamura H, Yoshimura N
  • Journal Title: Jpn J Ophthalmol 54(1) Pages: 55-60
  • NAID: 10027203479
  • Peer Reviewed
• [Journal Article] Prognostic factors of vitreous hemorrhage secondary to exudative age-related macular degeneration. (2010)
  • Author(s): Hasegawa T, Otani A, Sasahara M, Gotoh N, Ooto S, Tamura H, Yamashiro K, Tsujikawa A, Yoshimura N
  • Journal Title: Am J Ophthalmol 149(2) Pages: 322-329
  • NAID: 120001823762
  • Peer Reviewed
• [Journal Article] PUNCTATE HYPERFLUORESCENT SPOTS ASSOCIATED WITH CENTRAL SEROUS CHORIORETINOPATHY AS SEEN ON INDOCYANINE GREEN ANGIOGRAPHY (2010)
  • Author(s): Tsujikawa A, Ojima Y, Yamashiro K, Ooto S, Tamura H, Nakagawa S, Yoshimura N
  • Journal Title: Retina (in press)
  • Peer Reviewed
• [Journal Article] Sterile endophthalmitis after intravitreal injection of bevacizumab obtained from a single batch. (2010)
  • Author(s): Yamashiro K, Tsujikawa A, Miyamoto K, Oh H, Otani A, Tamuara H, Ooto S, Sasahara M, Iwama D, Yoshimura N
  • Journal Title: Retina 30(3) Pages: 485-90
  • NAID: 120002949750
  • Peer Reviewed
• [Journal Article] ARMS2/HTRA1 and CFH polymorphisms are not associated with choroidal neovascularization in highly myopic eyes of the elderly Japanese population. (2010)
  • Author(s): Nakanishi H, Gotoh N, Yamada R, Yamashiro K Shimada N, Ohno-Matsui K, Mochizuki M, Saito M, Saito K, Iida T, Matsuda F,, Otani A, Hayashi H, Tsujikawa A, Yoshimura N
  • Journal Title: Eye (Lond) (in press)
  • Peer Reviewed
• [Journal Article] Genetic variants near TIMP3 and high-density lipoprotein-associated loci influence susceptibility to age-related macular degeneration. (2010)
  • Author(s): Chen W, Stambolian D, Edwards AO, Branham KE, Othman M, Jakobsdottir J, Tosakulwong N, Pericak-Vance MA, Campochiaro PA, Klein ML, Tan PL, Conley YP, Kanda A, Kopplin L, Li Y, Augustaitis KJ, Karoukis AJ, Scott WK, Agarwal A, Kovach JL, Schwartz SG, Postel EA, Brooks M, Baratz KH, Brown WL ; Complications of Age-Related Macular Degeneration Prevention Trial (CAPT) Research Group, Brucker AJ, Orlin A, Brown G, Ho A, Regillo C, Donoso L, Tian L, Kaderli B, Hadley D, Hagstrom SA, Peachey NS, Klein R, Klein BE, Gotoh N, Yamashiro K, Ferris Iii F, Fagerness JA, Reynolds R, Farrer LA, Kim IK, Miller JW, Corton M, Carracedo A, Sanchez-Salorio M, Pugh EW, Doheny KF, Brion M, Deangelis MM, Weeks DE, Zack DJ, Chew EY, Heckenlively JR, Yoshimura N, Iyengar SK, Francis PJ, Katsanis N, Seddon JM, Haines JL, Gorin MB, Abecasis GR, Swaroop A.
  • Journal Title: Proc Natl Acad Sci USA (in press)
  • Peer Reviewed
• [Journal Article] Restoration of outer segments of foveal photoreceptors after resolution of central serous chorioretinopathy. (2010)
  • Author(s): Ojima Y, Tsujikawa A, Yamashiro K, Ooto S, Tamura H, Yoshimura N.
  • Journal Title: Jpn J Ophthalmol. (in press)
  • NAID: 10027203479
  • Peer Reviewed
• [Journal Article] Prognostic factors of vitreous hemorrhage secondary to exudative age-related macular degeneration. (2010)
  • Author(s): Hasegawa T, Otani A, Sasahara M, Gotoh N, Ooto S, Tamura H, Yamashiro K, Tsujikawa A, Yoshimura N.
  • Journal Title: Am J Ophthalmol. 149 Pages: 322-329
  • NAID: 120001823762
  • Peer Reviewed
• [Journal Article] PUNCTATE HYPERFLUORESCENT SPOTS ASSOCIATED WITH CEN TRAL SEROUS CHORIORETINOPATHY AS SEEN ON INDOCYANINE GREEN ANGIOGRAPHY. (2010)
  • Author(s): Tsujikawa A, Ojima Y, Yamashiro K, Ooto S, Tamura H, Nakagawa S, Yoshimura N.
  • Journal Title: Retina 30(in press)
  • Peer Reviewed
• [Journal Article] Sterile endophthalmitis after intravitreal injection of bevacizumab obtained from a single batch. (2010)
  • Author(s): Yamashiro K, Tsujikawa A, Miyamoto K, Oh H, Otani A, Tamuara H, Ooto S, Sasahara M, Iwama D, Yoshimura N.
  • Journal Title: Retina 30 Pages: 485-490
  • NAID: 120002949750
  • Peer Reviewed
• [Journal Article] ARMS2/HTRA1 and CFH polymorphisms are not associated with choroidal neovascularization in highly myopic eyes of the elderly Japanese population. (2010)
  • Author(s): Nakanishi H, Gotoh N, Yamada R, Yamashiro K, Otani A, Hayashi H, Tsujikawa A, Shimada N, Ohno-Matsui K, Mochizuki M, Saito M, Saito K, Iida T, Matsuda F, Yoshimura N.
  • Journal Title: Eye (in press)
  • Peer Reviewed
• [Journal Article] A genome-wide association analysis identified a novel susceptible locus for pathological myopia at 11q24.1. (2009)
  • Author(s): Nakanishi H, Yamada R, Gotoh N, Hayashi H, Yamashiro K, Shimada N, Ohno-Matsui K, Mochizuki M, Saito M, Iida T, Matsuo K, Tajima K, Yoshimura N, Matsuda F
  • Journal Title: PLoS Genet 5(9)
  • Peer Reviewed
• [Journal Article] Association between foveal photoreceptor integrity and visual outcome in neovascular age-related macular degeneration. (2009)
  • Author(s): Hayashi H, Yamashiro K, Tsujikawa A, Ota M, Otani A, Yoshimura N
  • Journal Title: Am J Ophthalmol 148(1) Pages: 83-9
  • Peer Reviewed
• [Journal Article] ARMS2 (LOC387715) variants in Japanese patients with exudative age-related macular degeneration and polypoidal choroidal vasculopathy. (2009)
  • Author(s): Gotoh N, Nakanishi H, Hayashi H, Yamada R, Otani A, Tsujikawa A, Yamashiro K Tamura H, Saito M, Saito K, Iida T, Matsuda F, Yoshimura N.
  • Journal Title: Am J Ophthalmol 147(6) Pages: 1037-41
  • Peer Reviewed
• [Journal Article] Absence of association between COL1A1 polymorphisms and high myopia in the Japanese population. (2009)
  • Author(s): Nakanishi H, Yamada R, Gotoh N, Hayashi H, Otani A, Tsujikawa A, Yamashiro K, Shimada N, Ohno-Matsui K, Mochizuki M, Saito M, Saito K, Iida T, Matsuda F, Yoshimura N
  • Journal Title: Invest Ophthalmol Vis Sci 50(2) Pages: 544-50
  • Peer Reviewed
• [Journal Article] A genome-wide association analysis identified a novel susceptible locus for pathological myopia at 11q24.1. (2009)
  • Author(s): Nakanishi H, Yamada R, Gotoh N, Hayashi H, Yamashiro K, Shimada N, Ohno-Matsui K, Mochizuki M, Saito M, Iida T, Matsuo K, Tajima K, Yoshimura N, Matsuda F.
  • Journal Title: PLoS Genet. 5
  • Peer Reviewed
• [Journal Article] Association between foveal photoreceptor integrity and visual outcome in neovascular age-related macular degeneration. (2009)
  • Author(s): Hayashi H, Yamashiro K, Tsujikawa A, Ota M, Otani A, Yoshimura N.
  • Journal Title: Am J Ophthalmol. 148 Pages: 83-89
  • Peer Reviewed
• [Journal Article] ARMS2 (LOC387715) variants in Japanese patients with exudative age-related macular degeneration and polypoidal choroidal vasculopathy. (2009)
  • Author(s): Gotoh N, Nakanishi H, Hayashi H, Yamada R, Otani A, Tsujikawa A, Yamashiro K, Tamura H, Saito M, Saito K, Iida T, Matsuda F, Yoshimura N
  • Journal Title: Am J Ophthalmol. 147 Pages: 1037-1041
  • Peer Reviewed
• [Journal Article] Absence of association between COL1A1 polymorphisms and high myopia in the Japanese population. (2009)
  • Author(s): Nakanishi H, Yamada R, Gotoh N, Hayashi H, Otani A, Tsujikawa A, Yamashiro K, Shimada N, Ohno-Matsui K, Mochizuki M, Saito M, Saito K, Iida T, Matsuda F, Yoshimura N.
  • Journal Title: Invest Ophthalmol Vis Sci 50 Pages: 544-550
  • Peer Reviewed
• [Journal Article] Absence of association between COL1A1 polymorphisms and high myopia in the Japanese population (2009)
  • Author(s): Nakanishi H, Yamada R, Gotoh N, Hayashi H, Otani A, Tsujikawa A, Yamashiro K, Shimada N, Ohno-Matsui K, Mochizuki M, Saito M, Saito K, Iida T, Matsuda F, Yoshimura N.
  • Journal Title: Invest Ophthalmol Vis Sci 50 Pages: 544-550
  • Peer Reviewed
• [Journal Article] Recurrence of polypoidal choroidal vasculopathy after photodynamic therapy. (2008)
  • Author(s): Yamashiro K, Tsujikawa A, Nishida A, Mandai M, Kurimoto Y
  • Journal Title: Jpn J Ophthalmol 52(6) Pages: 457-62
  • NAID: 10025148855
  • Peer Reviewed
• [Journal Article] Recurrence of Polypoidal Chroidal Vasculopathy After Photodynamic Therapy (2008)
  • Author(s): Yamashiro K, Tsujikawa A, Nishida A, Mandai M, Kurimoto Y
  • Journal Title: Jpn J Ophthalmol 52 Pages: 457-462
  • Peer Reviewed
• [Presentation] ARMS2遺伝子A69S多型の迅速検出キットの開発および加齢黄斑変性診療における臨床応用. (2009)
  • Author(s): 山城健児、後藤謙元、中西秀雄、林寿子、仲田勇夫、三谷康正、吉村長久
  • Organizer: 第113回京都眼科学会
  • Place of Presentation: 京都
  • Year and Date: 2009-09-06
• [Presentation] ARMS2遺伝子A69S多型の迅速検出キットの開発および加齢黄斑変性診療における臨床応用 (2009)
  • Author(s): 山城健児、後藤謙元、中西秀雄、林寿子、仲田勇夫、三谷康正、吉村長久
  • Organizer: 第113回 京都眼科学会
  • Place of Presentation: 京都
  • Year and Date: 2009-09-06
• [Presentation] 網膜色素線条に生じた脈絡膜新生血管の特徴 (2009)
  • Author(s): 山城健児、中川聡子、辻川明孝、大谷篤史、田村寛、大音壮太郎、佐々原学、吉村長久
  • Organizer: 第113回 日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-16
• [Presentation] 網膜色素線条に生じた脈絡膜新生血管の特徴 (2009)
  • Author(s): 山城健児、中川聡子、辻川明孝、大谷篤史、田村寛、大音壮太郎、佐々原学、吉村長久
  • Organizer: 班会議
  • Place of Presentation: 名古屋市
  • Year and Date: 2009-01-07
• [Presentation] 網膜色素線条に生じた脈絡膜新生血管の特徴. (2009)
  • Author(s): 山城健児、中川聡子、辻川明孝、大谷篤史、田村寛、大音壮太郎、佐々原学、吉村長久
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京
• [Presentation] 光線力学療法を施行し長期経過観察した加齢黄斑変性の網膜断層所見と視力の相関. (2008)
  • Author(s): 山城健児、辻川明孝、林寿子、太田将文、田村寛、大谷篤史、吉村長久
  • Organizer: 第112回京都眼科学会
  • Place of Presentation: 京都
  • Year and Date: 2008-06-01
• [Presentation] 光線力学療法を施行し長期経過観察した加齢黄斑変性の網膜断層所見と視力の相関 (2008)
  • Author(s): 山城健児・辻川明孝・林寿子・太田将文・田村寛・大谷篤史・吉村長久
  • Organizer: 京都眼科学会
  • Place of Presentation: 京都市
  • Year and Date: 2008-06-01
• [Presentation] 網膜色素線条に生じた脈絡膜新生血管の特徴. (2008)
  • Author(s): 山城健児、中川聡子、辻川明孝、大谷篤史、田村寛、大音壮太郎、佐々原学、吉村長久
  • Organizer: 厚生労働省難治性疾患克服研究事業網膜脈絡膜・視神経萎縮症調査研究班班会議
  • Place of Presentation: 名古屋
• [Presentation] 光線力学療法によって良好な視力が得られた加齢黄斑変性の長期予後. (2008)
  • Author(s): 山城健児、西田明弘、万代道子、栗本康夫
  • Organizer: 第112回日本眼科学会総会
  • Place of Presentation: 神奈川
• [Book] みるみるわかる目の疾患 (2009)
  • Author(s): 山城健児
  • Total Pages: 8
  • Publisher: メディカ出版