| Project/Area Number |
20791299
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatric surgery
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| Research Institution | Osaka University |
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Principal Investigator |
TAKAMA Yuichi Osaka University, 医学部附属病院, 医員 (50467560)
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| Co-Investigator(Renkei-kenkyūsha) |
OUE Takaharu 大阪大学, 医学系研究科, 講師 (50314315)
FUKUZAWA Masahiro 大阪大学, 医学系研究科, 教授 (60165272)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | WT1遺伝子 / サイレンシング / 分化誘導療法 / SiRNA / 腎芽腫 / 神経芽腫 / 横紋筋肉腫 / 肝芽腫 / サィレンシング / Wilms腫瘍 / siRNA / 分化誘導 |
|---|
| Research Abstract |
Immunohistochemical staining revealed that in neuroblastoma (NB), differentiating cells showed strong cytoplasmic staining. Expression of WT1 was higher in mature ganglionic cells, and in the immunohistochemical analysis, the WT1 positivity for ganglioneuromas was significantly higher than that for neuroblastomas. Knockdown of WT1 gene promoted the proliferation of NB69 cells (P<.01).The WT1 may govern cell differentiation and suppress cell proliferation in NB. The WT1 does not act as an oncogene, but it may participate in the maturation of NB, therefore can be a potential therapeutic target for cell differentiation.
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