Effects of WT1 gene knockdown in Wilms tumor

• Project/Area Number: 20791299
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pediatric surgery
• Research Institution: Osaka University
• Principal Investigator: TAKAMA Yuichi Osaka University, 医学部附属病院, 医員 (50467560)
• Co-Investigator(Renkei-kenkyūsha): OUE Takaharu 大阪大学, 医学系研究科, 講師 (50314315) ; FUKUZAWA Masahiro 大阪大学, 医学系研究科, 教授 (60165272)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: WT1遺伝子 / サイレンシング / 分化誘導療法 / SiRNA / 腎芽腫 / 神経芽腫 / 横紋筋肉腫 / 肝芽腫 / サィレンシング / Wilms腫瘍 / siRNA / 分化誘導

Research Abstract

Immunohistochemical staining revealed that in neuroblastoma (NB), differentiating cells showed strong cytoplasmic staining. Expression of WT1 was higher in mature ganglionic cells, and in the immunohistochemical analysis, the WT1 positivity for ganglioneuromas was significantly higher than that for neuroblastomas. Knockdown of WT1 gene promoted the proliferation of NB69 cells (P<.01).The WT1 may govern cell differentiation and suppress cell proliferation in NB. The WT1 does not act as an oncogene, but it may participate in the maturation of NB, therefore can be a potential therapeutic target for cell differentiation.

Research Products

• [Journal Article] The role of WT1 gene in neuroblastoma. (2011)
  • Author(s): Wang J, Oue T, Uehara S, et al
  • Journal Title: J Pediatr Surg. 46 Pages: 326-331
• [Journal Article] A Case Report of Cystic Dilatation of the Intrahepatic Biliary Ducts after Kasai Procedure (2009)
  • Author(s): Yuichi Takama, Takehisa Ueno, Natsumi Tanaka, Hideki Soh, Keisuke Nose, Masahiro Fukuzawa
  • Journal Title: Biliary Atresia -Proceedings of the 35th Conference of the Japanese Biliary Atresia Society 1 Pages: 70-73
• [Journal Article] 稀な先天性頚部皮膚病変である正中頚裂congenital midline cervical cleftの2例 (2009)
  • Author(s): 高間勇一, 奥山宏臣, 窪田昭男, 中岡達雄, 東孝, 中村哲郎, 福澤正洋
  • Journal Title: 日本小児外科学会雑誌 45巻 Pages: 744-748
  • NAID: 110007383182
  • Peer Reviewed
• [Journal Article] Expression of Wilms Tumor 1 (WT1) Gene in a Variety of Pediatric Tumors.
  • Author(s): Oue T, Uehara S, Yamanaka H, Takama U, Fukuzawa M
  • Journal Title: J Pediatr Surg (in Press)
• [Presentation] Expression of Wilms Tumor 1 (WT1) Gene in a Variety of Pediatric Tumors. (2011)
  • Author(s): Oue T, Uehara S, Yamanaka H, Takama U, Fukuzawa M
  • Organizer: 44th Annual Meeting of the Pacific Association of Pediatric Surgeons.
  • Place of Presentation: Cancun, Mexico
• [Presentation] Effects of a calcineurin inhibitor, FK506, and a CCR5/CXCR3 antagonist, TAK-779, in a rat small intestinal transplantation model (2010)
  • Author(s): Takama Y
  • Organizer: International Congress of The Transplantation Society
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2010-08-16
• [Presentation] The Roleof WT1 gene in neuroblastoma. (2010)
  • Author(s): OueT, Wang J, Uehara S, Yamanaka H, Oji Y, Fukuzawa M
  • Organizer: 57th Annual Congress of the British Association of the Paediatric Surgeons(BAPS)
  • Place of Presentation: Aberdeen, UK
• [Presentation] Effects of a calcineurin inhibitor, FK506, and a CCR5/CXCR3 antagonist, TAK-779, in a rat small intestinal transplantation model (2009)
  • Author(s): Takama Y, Miyagawa S, Xu H, Ueno T, Firdawes S, Yamamoto A, Ikeda K, Fukuzawa M.
  • Organizer: XIth International Small Bowel Transplant Symposium
  • Place of Presentation: Bologna, Italy
  • Year and Date: 2009-09-11