Molecular-biological study for clarifying the etiology of lung hypoplasia of Gja1 knockout mice

• Project/Area Number: 20791300
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pediatric surgery
• Research Institution: Kyushu University
• Principal Investigator: NAGATA Kouji Kyushu University, 大学院・医学研究院・小児外科学分野, 助教 (20419568)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 小児呼吸器外科学 / Gja1 / connexin43 / 肺低形成 / 肺発達 / IGF / ニトロフェン / 横隔膜ヘルニア / Gja-1 / gap junction / 低形成肺

Research Abstract

Gja1 (Cx43) knockout mice die after birth due to hypoplastic lungs. Adult hetero Cx43 mice were mated. Newborn mice were divided into the wild group and the knockout group. H.E. staining in the knockout mice showed narrow airspaces and thicker interalveolar septae. The m-RNA expression of AQP-5, Sp-C and a-SMA was significantly lower in knockout mice than that in the wild mice. Immunohistochemical staining in all markers (AQP-5, Sp-C and a-SMA) showed the same result to the m-RNA expression.

Research Products

• [Journal Article] Effect of insulin like growth factors on lungdevelopment in a nitrofen induced CDH model rat. (2010)
  • Author(s): Esumi G, Masumoto K, Teshiba R, Nagata K, Taguchi Tomoaki, et al.
  • Journal Title: Pediatr Surg Int. 27 Pages: 187-192
• [Journal Article] Identification of TCTE3 as a gene responsible for congenital diaphragmatic hernia using a high-resolution single-nucleotide polymorphism array. (2010)
  • Author(s): Teshiba R, Masumoto K, Esumi G, Nagata K, Tomoaki Taguchi, et al.
  • Journal Title: Pediatr Surg Int. 27 Pages: 193-198
• [Journal Article] Effect of insulin like growth factors on lungdevelopment in a nitrofen induced CDH model rat. (2010)
  • Author(s): Esumi G, Masumoto K, Teshiba R, Nagata K, et al.
  • Journal Title: Pediatric Surgery International Volume: 27 Pages: 187-192
  • Peer Reviewed
• [Journal Article] Identification of TCTE3 as a gene responsible for congenital diaphragmatic hernia using a high-resolution single-nucleotide polymorphism array (2010)
  • Author(s): Teshiba R, Masumoto K, Esumi G, Nagata K, et al.
  • Journal Title: Pediatric Surgery International Volume: 27 Pages: 193-198
  • Peer Reviewed
• [Journal Article] Outcome and treatment in an antenatally diagnosed CCAM of the lung. (2009)
  • Author(s): Nagata K, Masumoto K, Tesiba R, Esumi G, Tsukimori K, Wake N, Taguchi T.
  • Journal Title: Pediatr Surg Int 25 Pages: 753-757
• [Journal Article] 新生児腸間膜裂孔ヘルニアの1例 (2009)
  • Author(s): 永田公二、増本幸二、田尻達郎、田口智章
  • Journal Title: 日本小児外科学会雑誌 45 Pages: 1055-1059
• [Journal Article] Cx43 play important roles in the lung development. (2009)
  • Author(s): Kouji Nagata, Kouji Masumoto, Risa Tesiba, Genshirou Esumi, Keigo Yoshizaki, Satoshi Fukumoto, Kazuaki Nonaka, Tomoaki Taguchi.
  • Journal Title: J Pediatr Surg 44 Pages: 2296-2301
• [Journal Article] 当科にて経験した超低出生体重児の消化管穿孔14例に関する検討 (2009)
  • Author(s): 永田公二, 江角元史郎, 手柴理沙, 木下義晶, 増本幸二, 高畑靖, 原寿郎, 田口智章
  • Journal Title: 日本周産期新生児学会雑誌 45巻 Pages: 1104-1107
  • NAID: 10029090800
• [Journal Article] Outcome and treatment in an antenatally diagnosed congenital cystic a denomatoid malformation of the lung (2009)
  • Author(s): Kouji Nagata, Kouji Masumoto, Risa Tesiba, Genshirou Esumi, Kiyomi Tsukimori, Norio Wake, Tomoaki Taguchi
  • Journal Title: Pediatric Surgery International 25巻 Pages: 753-757
  • Peer Reviewed
• [Journal Article] 新生児腸間膜裂孔ヘルニアの1例 (2009)
  • Author(s): 永田公二、増本幸二、田尻達郎、田口智章
  • Journal Title: 日本小児外科学会雑誌 45巻 Pages: 1055-1059
  • Peer Reviewed
• [Journal Article] Cx43 play important roles in the lung development (2009)
  • Author(s): Kouii Nagata, Kouji Masumoto, Risa Tesiba, Genshirou Esumi, Keigo Yoshizaki, Satoshi Fukumoto, Kazuaki Nonaka, Tomoaki Taguchi
  • Journal Title: Journal of Pediatric Surgery 44巻 Pages: 2296-2301
  • Peer Reviewed
• [Journal Article] メッケル憩室を伴った新生児腸管部分拡張症の1例 (2008)
  • Author(s): 上杉達, 田尻達郎, 永田公二, 家入里志, 増本幸二, 田口智章
  • Journal Title: 小児外科 40巻 Pages: 721-724
• [Journal Article] 横隔膜ヘルニアの治療ストラテジー低出生体重児に合併した先天性横隔膜ヘルニアの治療成績 (2008)
  • Author(s): 増本幸二, 手柴理沙, 江角元史郎, 永田公二, 高畑靖, 原寿郎, 月森清己, 和氣徳夫, 田口智章
  • Journal Title: 日本周産期・新生児医学会雑誌 44巻 Pages: 1064-1068
• [Journal Article] Risk of respiratory syncytial virus in survivors with severe congenital diaphragmatic hernia. (2008)
  • Author(s): Masumoto K, Nagata K, Uesugi T, Yamada T, Kinjo T, Hikino S, Hara T, Taguchi T.
  • Journal Title: Pediatr Int. 50 Pages: 459-463
  • NAID: 10025344254
• [Journal Article] Chronological change in intramural components in severe proximally dilated jejunal atresia : an immunohistochemical study. (2008)
  • Author(s): Masumoto K, Akiyoshi J, Nagata K, Uesugi T, Taguchi S, Tajiri T, Taguchi T.
  • Journal Title: J Pediatr Gastroenterol Nutr. 46 Pages: 602-606
• [Journal Article] メッケル憩室を伴った新生児腸管部分拡張症の1例 (2008)
  • Author(s): 上杉達, 田尻達郎, 永田公二, 家入里志, 増本幸二, 田口智章
  • Journal Title: 小児外科 40 Pages: 721-724
  • Peer Reviewed
• [Journal Article] 横隔膜ヘルニアの治療ストラテジー低出生体重児に合併した先天性横隔膜ヘルニアの治療成績 (2008)
  • Author(s): 増本幸二, 手柴理沙, 江角元史郎, 永田公二, 高畑靖, 原寿郎, 月森清己, 和氣徳夫, 田口智章
  • Journal Title: 日本周産期・新生児医学会雑誌 44 Pages: 1064-1068
  • Peer Reviewed
• [Journal Article] Risk of respiratory syncytial virus in survivors with severe congenital diaphragmatic hernia (2008)
  • Author(s): Masumoto K, Naqata K, Uesugi T, Yamada T, Kinjo T, Hikino S, H ara T, Taguchi T
  • Journal Title: Pediatr Int 50 Pages: 459-463
  • Peer Reviewed
• [Journal Article] Chronological change in intramural components in severe proximally dilated jejunal atresia : an immunohistochemical study (2008)
  • Author(s): Masumoto K, Akiyoshi J, Naciata K, Uesugi T, Taguchi S, Tajiri T, Taguchi T
  • Journal Title: J Pediatr Gastroenterol Nutr 46 Pages: 602-606
  • Peer Reviewed
• [Presentation] Review of 17 cases of extremely low birth weight infants associated with intestinal perforation. (2011)
  • Author(s): Nagata K, Teshiba R, Masumoto K, Esumi G, Kinoshita Y, Tomoaki Taguchi.
  • Organizer: 23rd International Symposium on Pediatric Surgical Research.
  • Place of Presentation: 東京
  • Year and Date: 2011-09-14
• [Presentation] 当科で経験したlong gap食道閉鎖症の長期経過に対する検討 (2011)
  • Author(s): 永田公二、江角元史郎、手柴理沙、木下義晶、増本幸二、田口智章
  • Organizer: 第46回周産期新生児学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-07-13
• [Presentation] Hypoganglionosis長期経過観察中に低P血症、低Mg血症を発症した1成人例 (2011)
  • Author(s): 永田公二、江角元史郎、手柴理沙、増本幸二、田口智章
  • Organizer: 第47回日本外科代謝栄養学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-07-09
• [Presentation] 両側横隔膜ヘルニアの1例 (2011)
  • Author(s): 永田公二、江角元史郎、手柴理沙、木下義晶、増本幸二、田口智章
  • Organizer: 第47回日本小児外科学会
  • Year and Date: 2011-06-11
• [Presentation] Axillary crease incision for pediatric thoracotomy. (2010)
  • Author(s): Nagata K, Teshiba R, Masumoto K, Esumi G, Kinoshita Y, Tomoaki Taguchi.
  • Organizer: 3rd World Congress of Pediatric Surgery.
  • Place of Presentation: New Delhi, India
  • Year and Date: 2010-10-22
• [Presentation] Axillary crease incision for pediatric thoracotomy (2010)
  • Author(s): Nagata Kouji, 他
  • Organizer: 3^<rd> World Congress of Pediatric Surgery
  • Place of Presentation: Taji Palace Hotel(インド)
  • Year and Date: 2010-10-22
• [Presentation] Review of 17 cases of extremely low birth weight infants associated with intestinal perforation (2010)
  • Author(s): Nagata Kouji, 他
  • Organizer: 23rd International Symposium on Pediatric Surgical Research
  • Place of Presentation: 都市センターホテル(東京都)
  • Year and Date: 2010-09-14
• [Presentation] 当科で経験したlong gap食道閉鎖症の長期経過に対する検討 (2010)
  • Author(s): 永田公二, 他
  • Organizer: 第46回日本周産期・新生児医学会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2010-07-13
• [Presentation] Hypoganglionosis長期経過観察中に低P血症、低Mg血症を発症した1成人例 (2010)
  • Author(s): 永田公二, 他
  • Organizer: 第47回日本外科代謝栄養学会
  • Place of Presentation: 神奈川県民ホール(神奈川県)
  • Year and Date: 2010-07-09
• [Presentation] 両側横隔膜ヘルニアの1例 (2010)
  • Author(s): 永田公二, 他
  • Organizer: 第47回日本小児外科学会
  • Place of Presentation: 愛知県産業労働センター ウインク愛知(愛知県)
  • Year and Date: 2010-06-11
• [Presentation] 難治性手術創に対する陰圧閉鎖療法の適応 (2010)
  • Author(s): 永田公二、江角元史郎、手柴理沙、木下義晶、増本幸二、田口智章、他
  • Organizer: 第24回小児ストーマ・排泄管理研究会
  • Place of Presentation: 埼玉
  • Year and Date: 2010-04-24
• [Presentation] 難治性手術創に対する陰圧閉鎖療法の適応 (2010)
  • Author(s): 永田公二, 他
  • Organizer: 第24回小児ストーマ・排泄管理研究会
  • Place of Presentation: 埼玉会館(埼玉県)
  • Year and Date: 2010-04-24
• [Presentation] 当科における横隔膜ヘルニアの治療戦略 (2010)
  • Author(s): 永田公二、江角元史郎、手柴理沙、木下義晶、増本幸二、田口智章、他
  • Organizer: 第110回日本外科学会定期学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-09
• [Presentation] 当科における横隔膜ヘルニアの治療戦略 (2010)
  • Author(s): 永田公二, 他
  • Organizer: 第110回日本外科学会定期学術集会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2010-04-09
• [Presentation] 小児嚢胞性肺疾患に対する腋窩皺切開の検討 (2009)
  • Author(s): 永田公二, 江角元史郎, 手柴理沙, 木下義晶, 田口智章
  • Organizer: 第20回小児呼吸器外科研究会
  • Place of Presentation: 高崎
  • Year and Date: 2009-10-17
• [Presentation] 胸骨翻転術を施行した漏斗胸愚者の長期成績に関する検討 (2009)
  • Author(s): 永田公二, 江角元史郎, 手柴理沙, 木下義晶, 田口智章
  • Organizer: 第39回九州小児外科研究会
  • Place of Presentation: 沖縄
  • Year and Date: 2009-08-22
• [Presentation] 胸骨翻転術を施行した漏斗胸患者の長期成績に関する検討 (2009)
  • Author(s): 永田公二, 江角元史郎, 手柴理沙, 木下義晶, 田口智章
  • Organizer: 第39回九州小児外科研究会
  • Place of Presentation: 沖縄
  • Year and Date: 2009-08-02
• [Presentation] 当科にて経験した超低出生体重児の消化管穿孔14例に関する検討 (2009)
  • Author(s): 永田公二, 江角元史郎, 手柴理沙, 木下義晶, 増本幸二, 高畑靖, 原寿郎, 田口智章
  • Organizer: 第44回日本周産期新生児学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-13
• [Presentation] Cx43 plays important roles in lung development. (2009)
  • Author(s): Kouji Nagata, Genshirou Esumi, Risa Teshiba, Kouji Masumoto, Keigo Yoshizaki, Satoshi Fukumoto, Kazuaki Nonaka, Tomoaki Taguchi.
  • Organizer: 42nd Annual Meeting of Pacific Association of Pediatric Surgeons.
  • Place of Presentation: Hong Kong
  • Year and Date: 2009-05-11
• [Presentation] Cx43 plays important roles in lung development. (2009)
  • Author(s): Kouji Nagata, Genshirou Esumi, Risa Teshiba, Kouji Masumoto, Keigo Yoshizaki, Satoshi Fukumoto, Kazuaki Nonaka, Tomoaki Taguchi
  • Organizer: 42nd Annual Meeting of Pacific Association of Pediatric Surgeons
  • Place of Presentation: Hong Kong
  • Year and Date: 2009-05-11
• [Presentation] 虫垂切除術時に偶発的に発見された回腸異所性胃粘膜の1例 (2008)
  • Author(s): 永田公二, 藤花菜, 増本幸二, 田口智章
  • Organizer: 第452回小児科学会福岡地方会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-13
• [Presentation] The postnatal outcome and timing of surgery for an antenatally diagnosed CCAM of the lung. (2008)
  • Author(s): Kouji Nagata, Risa Tesiba, Genshirou Esumi, Kouji Masumoto, Tomoaki Taguchi.
  • Organizer: 21th Congress of Asian Association of Pediatric Surgeons.
  • Place of Presentation: Bankok, Thai
  • Year and Date: 2008-11-17
• [Presentation] The postnatal outcome and timing of surgery for an antenatally diagnosed congenital cystic adenomatoid malformation of the lung (2008)
  • Author(s): Kouji Nagata, Risa Tesiba, Gens hirouEsumi, Kouji Masumoto and Tomoaki Taguchi
  • Organizer: 21th Congress of Asian Association of Pediatric Surgeons
  • Place of Presentation: Bankok, Thai
  • Year and Date: 2008-11-17
• [Presentation] 当科にて経験した超低出生体重児の消化管穿孔13例に関する検討 (2008)
  • Author(s): 永田公二, 江角元史郎, 手柴理沙, 増本幸二, 田口智章
  • Organizer: 第28回日本小児内視鏡外科手術手技研究会
  • Place of Presentation: 大阪
  • Year and Date: 2008-11-09
• [Presentation] 出生前診断された先天性嚢胞性腺腫様奇形(CCAM)症例に関する検討 (2008)
  • Author(s): 永田公二, 江角元史郎, 手柴理沙, 増本幸二, 田口智章
  • Organizer: 第44回日本周産期新生児学会
  • Place of Presentation: 横浜
  • Year and Date: 2008-07-14
• [Presentation] 当科における出生前診断された先天性嚢胞性腺腫様奇形(CCAM)症例に関する検討 (2008)
  • Author(s): 永田公二, 江角元史郎, 手柴理沙, 増本幸二,田口智章
  • Organizer: 第45回日本小児外科学会学術集会
  • Place of Presentation: 筑波
  • Year and Date: 2008-05-28