Survival of salivary pleomorphic adenoma cells in hypoxic condition
| Project/Area Number |
20791331
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
MARUYAMA Satoshi Niigata University, 医歯学総合病院, 講師 (30397161)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 口腔病理学 / 唾液腺多形性腺腫 / 低酸素 / HIF-1α / VEGF / pVHL / SM-AP / ACC |
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| Research Abstract |
Salivary pleomorphic adenoma is histopathologically characaterized by its colorful stroma including myxoid one which is poorly vascularized. Pleomorphic adenoma (PA) cells embedded in such poorly-vasculaized stromata are supposed to have their own device to survive in hypoxic conditions. To understand the hypoxia-dependent manner of cellular proliferation, we determined both protein and gene expression levels of hypoxia-inducible factor 1α (HIF-1α), vascular endothelial growth factor (VEGF), and von Hippel-Lindau (vHL) protein which degrades HIF-1α in SM-AP cells originated from a pleomorphic adenoma. They showed more enhanced gene expression levels for VEGF but not for those for HIF-1α under hypoxic conditions. In contrast, HIF-1α and VEGF protein levels were kept higher in hypoxic conditions than in aerobic ones. SM-AP cells had lower expression levels for the vHL gene. On the one hand, their VEGF protein levels were kept lower in hypoxic conditions than in aerobic ones. The results indicate that PA cells are able to proliferate in the hypoxic condition because of the accumulation of HIF-1α probably due to repressed levels of vHL.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Matrix metalloproteinase 7 and perlecan in oral epithelial dysplasia and carcinoma in-situ: an aid for histopathological recognition of their cell proliferation centers2009
Author(s)
Tilakaratne WM, Kobayashi T, Ida-Yonemochi H, Swelam W, Yamazaki M, Mikami T, Alvarado CG, Shahidul AM, Maruyama S, Cheng J, Saku T
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Journal Title
J Oral Pathol Med 38(4)
Pages: 348-355
Related Report
Peer Reviewed
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