Identification of a novel tumor suppressor gene at 1p36 chromosomal region in oral squamous cell carcinomas.

• Project/Area Number: 20791337
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Okayama University
• Principal Investigator: TAMAMURA Ryo Okayama University, 大学院・医歯薬学総合研究科, 助教 (00403494)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 癌抑制遺伝子 / 扁平上皮癌 / 口腔癌 / LOH解析 / LOH解折 / 発癌

Research Abstract

To investigate the presence of tumor suppressor gene (TSG) at 1p36 chromosomal region, I have analyzed loss of heterozygosity (LOH) in oral squamous cell carcinoma (OSCC). As a result of this study three frequently deleted regions that harbor some potential TSG involved in OSCC carcinogenesis were identified. I focused on RIZ gene which existed in the most highest deletion region, and have done genetic analysis. Two polymorphisms of RIZ gene have been found in OSCC.

Research Products

• [Journal Article] Tumor-specific mutation and down-regulation of ING5 detected in oral squamous cell carcinoma. (2010)
  • Author(s): Cengiz B, Gunduz E, Gunduz M, Beder LB, Tamamura R, Bagci C, Yamanaka N, Shimizu K, Nagatsuka H.
  • Journal Title: Int J Cancer. 127(9) Pages: 2088-94
  • Peer Reviewed
• [Journal Article] Frequent allelic loss of Dkk-1 locus (10q11.2) is related with low distant metastasis and better prognosis in head and neck squamous cell carcinomas. (2010)
  • Author(s): Katase N, Gunduz M, Beder LB, Gunduz E, Al Sheikh Ali M, Tamamura R, Yaykasli KO, Yamanaka N, Shimizu K, Nagatsuka H.
  • Journal Title: Cancer Invest. 28(1) Pages: 103-10
  • Peer Reviewed
• [Journal Article] Allelic loss of the ING gene family loci is a frequent event in ameloblastoma. (2010)
  • Author(s): Borkosky SS
  • Journal Title: Oncol Res. Volume: 18 Pages: 509-518
  • Peer Reviewed
• [Journal Article] Tumor-specific mutation and down-regulation of ING5 detected in oral squamous cell carcinoma. (2010)
  • Author(s): Cengiz B
  • Journal Title: Int J Cancer. Volume: 127 Pages: 2088-2094
  • Peer Reviewed
• [Journal Article] Expression and mutation analysis of her2 in head and neck squamous cell carcinoma. (2010)
  • Author(s): Ali MA
  • Journal Title: Cancer Invest. Volume: 28 Pages: 495-500
  • Peer Reviewed
• [Journal Article] Clinico-pathological evaluation of oral melanotic macule, oral pigmented nevus and oral mucosal melanoma. (2010)
  • Author(s): Rivera RB
  • Journal Title: J Hard Tissue Biology. Volume: 19 Pages: 57-64
  • Peer Reviewed
• [Journal Article] Frequent allelic loss of Dkk-1 locus (10q11.2) is related with low distant metastasis and better prognosis in head and neck squamous cell carcinomas. (2010)
  • Author(s): Katase N
  • Journal Title: Cancer Invest. 28 Pages: 103-110
  • Peer Reviewed
• [Journal Article] Potential usage of ING family members in cancer diagnostics and molecular therapy. (2009)
  • Author(s): Gunduz M, Demircan K, Gunduz E, Katase N, Tamamura R, Nagatsuka H.
  • Journal Title: Curr Drug Targets. 10(5) Pages: 465-76
  • Peer Reviewed
• [Journal Article] Potential usage of ING family members in cancer diagnostics and molecular therapy. (2009)
  • Author(s): Gunduz M
  • Journal Title: Curr Drug Targets. 10 Pages: 465-476
  • Peer Reviewed
• [Journal Article] Secreted frizzled related protein (sFRP)-2 inhibits bone formation and promotes cell proliferation in ameloblastoma. (2009)
  • Author(s): Sathi GA
  • Journal Title: Oral Oncol. 45 Pages: 856-860
  • Peer Reviewed
• [Journal Article] Heparanase and its related molecules in odontogenic tumors. (2009)
  • Author(s): Nagatsuka H
  • Journal Title: Oral Med Pathol. 13 Pages: 81-89
  • Peer Reviewed
• [Journal Article] Deletion at Dickkopf (dkk)-3 locus (11p15.2) is related with lower lymph node metastasis and better prognosis in head and neck squamous cell carcinomas. (2008)
  • Author(s): Katase N, Gunduz M, Beder L, Gunduz E, Lefeuvre M, Hatipoglu OF, Borkosky SS, Tamamura R, Tominaga S, Yamanaka N,Shimizu K, Nagai N, Nagatsuka H.
  • Journal Title: Oncol Res. 17(6) Pages: 273-82
  • Peer Reviewed
• [Journal Article] Deletion at Dickkopf (dkk)-3 locus (11p15.2) is related with lowerlymph node metastasis and better prognosis in head and neck squamous cell carcinomas (2008)
  • Author(s): Katase N
  • Journal Title: Oncol Res. 17(6) Pages: 273-282
  • Peer Reviewed
• [Journal Article] Stromal cells promote bone invasion by suppressing bone formation in ameloblastoma (2008)
  • Author(s): Sathi GSA
  • Journal Title: Histopathology. 53 Pages: 458-467
  • Peer Reviewed
• [Journal Article] Inhibitor of growth (ING) family : An emerging molecular target for cancer therapy (2008)
  • Author(s): Gunduz E
  • Journal Title: Journal of Hard Tissue Biology 17(1) Pages: 1-10
  • Peer Reviewed
• [Presentation] Fine deletion analysis of 1p36 region in oral squamous cell carcinomas (2010)
  • Author(s): Ryo Tamamura
  • Organizer: The 6th Asian Science Seminar
  • Place of Presentation: Taiwan
  • Year and Date: 2010-11-20
• [Presentation] LOH analysis of 1p36 region in oral squamous cell carcinoma (2010)
  • Author(s): Lefeuvre Mathieu
  • Organizer: 第52回歯科基礎医学会学術大会・総会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-21
• [Presentation] Three regions in 1p36 are preferentially deleted in oral squamous cell carcinoma (2010)
  • Author(s): Hidetsugu Tsujigiwa
  • Organizer: 15th International Congress of Ora1 Pathology and Medicine (IAOP)
  • Place of Presentation: Seoul
  • Year and Date: 2010-08-18
• [Presentation] Three regions in 1p36 are preferentially deleted in oral squamous cell carcinoma (2010)
  • Author(s): Hidetsugu Tsujigiwa
  • Organizer: 15th International Congress of Oral Pathology and Medicine
  • Place of Presentation: Seoul
  • Year and Date: 2010-08-18
• [Presentation] Application of loss of heterozygosity analysis for detection of tumor suppressor genes in head and neck squamous cell carcinoma (2010)
  • Author(s): Naoki Katase
  • Organizer: 15th International Congress of Oral Pathology and Medicine
  • Place of Presentation: Seoul
  • Year and Date: 2010-08-17
• [Presentation] 頭頸部扁平上皮癌におけるDkk-3蛋白発現解析 (2010)
  • Author(s): 片瀬直樹
  • Organizer: 第21回NPO法人日本臨床口腔病理学会総会・学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-08-01
• [Presentation] 頭頸部扁平上皮癌におけるDkk-3のヘテロ接合性消失(LOH)解析と蛋白発現の解析 (2010)
  • Author(s): 片瀬直樹
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-29
• [Presentation] 頭頸部扁平上皮癌におけるDkk-3遺伝子ファミリーのヘテロ接合性消失(LOH)と臨床病理学的特徴の相関 (2010)
  • Author(s): 松田寛之
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-28
• [Presentation] Expression pattern of stem cell markers in squamous cell carcinoma. (2009)
  • Author(s): Han Liu
  • Organizer: 第30回岡山歯学会総会・学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2009-11-08
• [Presentation] Threeregions are preferentially deleted in 1p36 region in oral squamous cell carcinoma (2009)
  • Author(s): Mathieu Lefeuvre
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-05-03
• [Presentation] Three regions are preferentially deleted in 1p36 region in oral squamous cell carcinoma. (2009)
  • Author(s): Mathieu Lefeuvre
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-05-03
• [Presentation] Identification of BRM as a candidate tumor suppressor from 9p24 region in head and neck squamous cell carcinomas. (2009)
  • Author(s): Gunduz E
  • Organizer: Third International Congress of Molecular Medicine
  • Place of Presentation: Istanbul
• [Presentation] 頭頸部扁平上皮癌におけるDkk-3のヘテロ接合性消失解析 (2009)
  • Author(s): 片瀬直樹
  • Organizer: 第20回日本臨床口腔病理学会総会・学術大会
  • Place of Presentation: 札幌
• [Presentation] Examination of loss of heterozygosity in lp 36 chromosomal region in oral squamous cell carcinoma (2008)
  • Author(s): Lefeuvre M
  • Organizer: 第50回歯科基礎医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-23
• [Presentation] Microsatellite analysis of ING tumor suppressor genes in ameloblastoma (2008)
  • Author(s): Borkosky SS
  • Organizer: 第17回硬組織再生生物学会
  • Place of Presentation: 徳島
  • Year and Date: 2008-08-30
• [Presentation] 上顎歯肉腫瘍 (2008)
  • Author(s): 片瀬直樹
  • Organizer: 第19回日本臨床口腔病理学会
  • Place of Presentation: 東京
  • Year and Date: 2008-08-21