| Project/Area Number |
20791339
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
KITAGAWA Masae Hiroshima University, 病院, 助教 (10403627)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | スポンディン / セメント芽細胞 / 歯周組織 / 再生 / セメント質 / BMP-7 / Smad1 / 5 / 8 / 石灰化 |
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| Research Abstract |
In this study, we used with recombinant F-spondin, F-spondin siRNA and F-spondin overexpressed cells to clarify the mechanism and examine the possibility of a novel therapy for periodontal tissue regeneration targeting F-spondin.Recombinant F-spondin increased mRNA expression of type I collagen and ALP of human periodontal ligament cells, but Runx2 and OCN were unchanged. We found that knock-down of F-spondin mRNA led to decreased mRNA expression of ALP and BSP and mineralized nodule formation. Overexpression of F-spondin increased BMP-7 mRNA and protein and induced to phosphorylation of Smad1/5/8. Taken together, these results suggest that F-spondin may play a critical role in human cementoblast mineralization via BMP-7 activation and lead to regeneration of periodontal tissue by promoting of the cementoblast differentiation.
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