Characterization of hemin-binding protein 35 (HBP35) in Porphyromonas gingivalis and analysis of its secretion mechanism

• Project/Area Number: 20791341
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Nagasaki University
• Principal Investigator: SHOJI Mikio Nagasaki University, 大学院・医歯薬学総合研究科, 助教 (10336175)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 歯周病細菌 / 歯周病 / 細菌 / 外膜蛋白 / 輸送機構

Research Abstract

Characterization of hemin-binding protein 35 (HBP35) in Porphyromonas gingivalis : the cellular distribution, thioredoxin activity and role in hemin utilization

Research Products

• [Journal Article] A protein secretion system linked to bacteroidete gliding motility and pathogenesis. (2010)
  • Author(s): Sato K, Naito M, Yukitake H, Hirakawa H, Shoji M, McBride MJ, Rhodes RG, Nakayama K.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 第107巻 Pages: 276-281
  • NAID: 120006984618
• [Journal Article] A protein secretion system linked to bacteroidete gliding motility and pathogenesis. (2010)
  • Author(s): Sato K, Naito M, Yukitake H, Hirakawa H, Shoji M, McBride MJ, Rhodes RG, Nakayama K.
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A. 107 Pages: 276-281
  • NAID: 120006984618
  • Peer Reviewed
• [Journal Article] Identification of a Gingipain-Sensitive Surface Ligand of Porphyromonas gingivalis that Induces TLR2- and TLR4-Independent NF-{kappa}B Activation in CHO Cells. (2009)
  • Author(s): Haruyama K, Yoshimura A, Naito M, Kishimoto M, Shoji M, Abiko Y, Hara Y, Nakayama K.
  • Journal Title: Infection and Immunity 第77巻 Pages: 4414-4420
• [Journal Article] Identification of a Gingipain-Sensitive Surface Ligand of Porphyromonas gingivalis that Induces TLR2- and TLR4-Independent NF-{kappa}B Activation in CHO Cells. (2009)
  • Author(s): Haruyama K, Yoshimura A, Naito M, Kishimoto M, Shoji M, Abiko Y, Hara Y, Nakayama K.
  • Journal Title: Infection and Immunity 77 Pages: 4414-4420
  • Peer Reviewed
• [Journal Article] Proteome analysis of Porphyromonas gingivalis cells placed in a subcutaneous chamber of mice. (2008)
  • Author(s): Yoshimura M, Ohara N, Kondo Y, Shoji M, Okano S, Nakano Y, Abiko Y, Nakayama K.
  • Journal Title: Oral Microbiology and Immunology 第23巻 Pages: 413-418
• [Journal Article] Determination of the genome sequence of Porphyromonas gingivalis strain ATCC 33277 and genomic comparison with strain W83 revealed extensive genome rearrangements in P. gingivalis. (2008)
  • Author(s): Naito M, Hirakawa H, Yamashita A, Ohara N, Shoji M, Yukitake H, Nakayama K, Toh H, Yoshimura F, Kuhara S, Hattori M, Hayashi T, Nakayama K.
  • Journal Title: DNA Research 第15巻 Pages: 215-225
  • NAID: 120006981768
  • Peer Reviewed
• [Journal Article] Determination of the genome sequence of Porphyromonas gingivalis strain ATCC 33277 and genomic comparison with strain W83 revealed extensive genome rearrangements in P. gingivalis (2008)
  • Author(s): 内藤真理子
  • Journal Title: DNA Research 15 Pages: 215-225
  • Peer Reviewed
• [Journal Article] Proteome analysis of Porphyromonas gingivalis cells placed in a subcutaneous chamber of mice (2008)
  • Author(s): 吉村満美子
  • Journal Title: Oral Microbiology and Immunology 23 Pages: 413-418
  • Peer Reviewed
• [Presentation] Porphyromonas gingivalisにおける外膜蛋白の輸送機構の解析 (2009)
  • Author(s): 庄子幹郎, 佐藤啓子, 成田由香, 雪竹英治, 内藤真理子, 中山浩次
  • Organizer: 日本細菌学会・若手コロセウム(III)
  • Place of Presentation: 宮崎
  • Year and Date: 2009-10-26
• [Presentation] Porphyromonas gingivalisのC-terminal domain (CTD) familyにおけるCTDの役割 (2009)
  • Author(s): 庄子幹郎、佐藤啓子、成田由香、雪竹英治、内藤真理子、中山浩次
  • Organizer: 日本歯科基礎医学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-10
• [Presentation] Porphyromonas gingivalis HBP35 protein is transported to the outer membrane by the PorT-dependent system and is involved in hemin utilization (2009)
  • Author(s): Mikio Shoji, Yasuko Shibata, Teruaki Shiroza, Hideharu Yukitake, Benjamin Peng, Yu-Yen Chen, Keiko Sato, Mariko Naito, Yoshimitsu Abiko, Eric C.Reynolds, Koji Nakayama
  • Organizer: Gordon research conference
  • Place of Presentation: アメリカ ニューハンプシヤー
  • Year and Date: 2009-08-03
• [Presentation] Porphyromonas gingivalisにおけるHBP35蛋白の局在化機構 (2009)
  • Author(s): 庄子幹郎
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-03-12
• [Presentation] Porphyromonas gingivalisにおける外膜蛋白の輸送機構の解析 (2009)
  • Author(s): 庄子幹郎, 佐藤啓子, 成田由香, 雪竹英治, 内藤真理子, 中山浩次
  • Organizer: 日本細菌学会・若手コロセウム
  • Place of Presentation: 宮崎
• [Presentation] Porphyromonas gingivalis HBP35 protein is transported to the outer membrane by the PorT-dependent system and is involved in hemin utilization (2009)
  • Author(s): Mikio Shoji, Yasuko Shibata, Teruaki Shiroza, Hideharu Yukitake, Benjamin Peng, Yu-Yen Chen, Keiko Sato, Mariko Naito, Yoshimitsu Abiko, Eric C. Reynolds, Koji
  • Organizer: Nakayama Gordon research conference
  • Place of Presentation: ニューハンプシヤー
• [Presentation] Porphyromonas gingivalisのC-terminal domain (CTD) familyにおけるCTDの役割 (2009)
  • Author(s): 庄子幹郎、佐藤啓子、成田由香、雪竹英治、内藤真理子、中山浩次
  • Organizer: Journal of Oral Biosciences
  • Place of Presentation: 新潟
• [Presentation] P. gingivalisにおけるHBP35蛋白の局在化機構 (2009)
  • Author(s): 庄子幹郎、佐藤啓子、雪竹英治、内藤真理子、中山浩次
  • Organizer: Japanese Journal of Bacteriology
  • Place of Presentation: 愛知
• [Presentation] Identification of binary proteins produced from Porphyromonas gingivalis hbp35 gene (2008)
  • Author(s): 庄子幹郎
  • Organizer: 第50会歯科基礎医学会学術大会・総会
  • Place of Presentation: TOC有明コンベンションホール
  • Year and Date: 2008-09-25
• [Presentation] Porphyromonas gingivalisにおける外膜蛋白HBP35の発現、機能、輸送機構の解析 (2008)
  • Author(s): 庄子幹郎
  • Organizer: 第2回細菌学・若手コロッセウム
  • Place of Presentation: 湘南国際村センター
  • Year and Date: 2008-08-03
• [Presentation] Identification of binary proteins produced from Porphyromonas gingivalis hbp35 gene. (2008)
  • Author(s): Mikio Shoji, Yasuko Shibata, Teruaki Shiroza, Hideharu Yukitake, Keiko Sato, Mariko Naito, Yasumitsu Abiko, Koji Nakayama
  • Organizer: Journal of Oral Biosciences
  • Place of Presentation: 東京
• [Presentation] Porphyromonas gingivalisにおける外膜蛋白HBP35の発現、機能、輸送機構の解析 (2008)
  • Author(s): 庄子幹郎
  • Organizer: 日本細菌学会・第2回若手コロセウム
  • Place of Presentation: 湘南
• [Remarks]
  • URL: http://www.nagasaki-u.ac.jp/main/gakujutsu/2009/gaku20091214.html